Mouse anti-SARS-CoV-2 N-Protein (1G1-F2)

CODE:  130-10781

Antigen Information

Accession Number:
  • QHD43423
Protein Name & Synonyms:
SARS-CoV-2 N-Protein
Target Species:
  • Virus

Product Specifications

Size:
20 ug, 100 ug, 200 ug, or 500 ug

Antibody Specifications

Applications:
Direct ELISA (recommended dilution = 1:320,000)
Sandwich ELISA: Functions in ELISA capture when paired with 130-10760.
Standard curve
Clone:
1G1-F2
Host Species:
Mouse
Preparation:
Immunogen was recombinant protein recombinant SARS-CoV-2 Nucleocapsid Protein derived from E.coli. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Reconstitution:
Product is supplied as a powder obtained from lyophilization of purified antibody in 1 x PBS. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Species Reactivity:
Virus
Clonality:
Monoclonal

Description

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways.

Storage

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.