Mouse anti-SARS-CoV-2 S1RBD (1F9-F2-C1-G9)

$115.00  
130-10845-20
+

Antigen Information

Accession Number:
  • QHD43416
Protein Name & Synonyms:
SARS-CoV-2 S1RBD
Target Species:
  • Virus

Product Specifications

Size:
20 ug, 100 ug, 200 ug, 500 ug, or 1000 ug

Antibody Specifications

Applications:
ELISA (recommended work dilution = 1:320,000)

Neutralization was measured by ability to inhibit entry of pseudovirus harboring the SARS-CoV-2 spike protein into A549 cells. This antibody was also demonstrated to block S1 RBD-ACE2 binding in vitro.
Clone:
1F9-F2-C1-G9
Host Species:
Mouse
Preparation:
Immunogen was recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by thiophilic medium with 2-mercaptopyridine.
Reconstitution:
Product is supplied as a powder obtained from lyophilization of purified antibody in 1 x PBS. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Species Reactivity:
Virus
Clonality:
Monoclonal

Description

It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Storage

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.