Protein Name & Synonyms:
Western blotting (1:1000), immunoprecipitation (1:500), and Immunohistochemistry (1:100). However, the optimal concentrations should be determined individually. The antibody recognizes 45-60 kDa human Tau-13. Reactivity to other species has not been tested.
FLJ31424 , FTDP 17 , FTDP17 , MSTD , MAPT
Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer's disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK3. This presents the possibility that miscues in GSK3 signaling contribute to the onset of Alzheimer's disease.
Synthetic peptide corresponding to the N-terminus of the longest isoform of human Tau
100 µl mouse ascites diluted in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
-20 °C. The shelf life is 12 months.