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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/Apoptosis Arrays/Mouse Apoptosis Array C1

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Assay Format

Pathway:

Akt Signaling
IGF Signaling
MAPK Signaling
NFkB Signaling
p53 Signaling
PI3K-AKT Signaling

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad Gene Symbol: Bad Bbc6 Gene ID: 12015 Accession #: Q61337 ELISA Kit: RayBio Custom Mouse BAD ELISA	bax Gene Symbol: Bax Gene ID: 12028 Accession #: Q07813	bcl-2 Gene Symbol: Bcl2 Bcl-2 Gene ID: 12043 Accession #: P10417 ELISA Kit: RayBio Custom Mouse bcl-2 ELISA	bcl-w Gene Symbol: Bcl2l2 Bclw Kiaa0271 Gene ID: 12050 Accession #: P70345 ELISA Kit: RayBio Mouse BCL-W ELISA	BID Gene Symbol: Bid Gene ID: 12122 Accession #: P70444 ELISA Kit: RayBio Mouse BID ELISA
BIM Gene Symbol: Bcl2l11 Bim Gene ID: 12125 Accession #: O54918	Caspase-3 Gene Symbol: Casp3 Cpp32 Gene ID: 12367 Accession #: P70677	Caspase-8 Gene Symbol: Casp8 Gene ID: 12370 Accession #: O89110	CD40 (TNFRSF5) Gene Symbol: Cd40 Tnfrsf5 Gene ID: 21939 Accession #: P27512 ELISA Kit: RayBio Mouse CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: Cd40lg Cd40l Tnfsf5 Gene ID: 21947 Accession #: P27548 ELISA Kit: RayBio Mouse CD40 Ligand ELISA
cIAP-2 Gene Symbol: Birc3 Birc2 Iap1 Gene ID: 11796 Accession #: O08863 ELISA Kit: RayBio Custom Mouse cIAP-2 ELISA	Cytochrome C	DR6 (TNFRSF21) Gene Symbol: Tnfrsf21 Dr6 Gene ID: 94185 Accession #: Q9EPU5 ELISA Kit: RayBio Custom Mouse Death Receptor 6 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Apt1 Tnfrsf6 Gene ID: 14102 Accession #: P25446 ELISA Kit: RayBio Mouse FAS/TNFRSF6/Apo-1 ELISA	Fas Ligand (TNFSF6) Gene Symbol: Faslg Apt1lg1 Cd95l Fasl gld Tnfsf6 Gene ID: 14103 Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA
HSP27 Gene Symbol: Hspb1 Hsp25 Hsp27 Gene ID: 15507 Accession #: P14602 ELISA Kit: RayBio Mouse HSP27 ELISA	HSP60 Gene Symbol: Hspd1 Hsp60 Gene ID: 15510 Accession #: P63038	HSP70 Gene Symbol: Hspa4 mCG_113604 Gene ID: 15525 Accession #: Q3U2G2 ELISA Kit: RayBio Custom Mouse Heat Shock Protein 70 ELISA	HTRA2 Gene Symbol: Htra2 Omi Prss25 Gene ID: 64704 Accession #: Q9JIY5	IGFBP-1 Gene Symbol: Igfbp1 Igfbp-1 Gene ID: 16006 Accession #: P47876 ELISA Kit: RayBio Custom Mouse IGFBP-1 ELISA
IGFBP-2 Gene Symbol: Igfbp2 Igfbp-2 Gene ID: 16008 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA	IGFBP-3 Gene Symbol: Igfbp3 Igfbp-3 Gene ID: 16009 Accession #: P47878 ELISA Kit: RayBio Mouse IGFBP-3 ELISA	IGFBP-4 Gene Symbol: Igfbp4 Igfbp-4 Gene ID: 16010 Accession #: P47879 ELISA Kit: RayBio Custom Mouse IGFBP-4 ELISA	IGFBP-5 Gene Symbol: Igfbp5 Igfbp-5 Gene ID: 16011 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA	IGFBP-6 Gene Symbol: Igfbp6 Igfbp-6 Gene ID: 16012 Accession #: P47880 ELISA Kit: RayBio Mouse IGFBP-6 ELISA
IGF-1 Gene Symbol: Igf1 Igf-1 Gene ID: 16000 Accession #: P05017 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGF-2 Gene Symbol: Igf2 Igf-2 Gene ID: 16002 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	p21 Gene Symbol: Cdkn1a Cip1 Waf1 Gene ID: 12575 Accession #: P39689 ELISA Kit: RayBio	p27/Kip1 (CDKN1B) Gene Symbol: PER27 P27 At3g01190 T4P13.12 Gene ID: 821314 Accession #: Q43735 ELISA Kit: RayBio Custom Mouse p27/Kip1 ELISA	p53 Gene Symbol: Tp53 P53 Trp53 Gene ID: 22059 Accession #: P02340
SMAC Gene Symbol: Diablo Smac Gene ID: 66593 Accession #: Q9JIQ3 ELISA Kit: RayBio Custom Mouse SMAC ELISA	Survivin (BIRC5) Gene Symbol: Birc5 Api4 Iap4 Gene ID: 11799 Accession #: O70201	TNF RI (TNFRSF1A) Gene Symbol: Tnfrsf1a Tnfr-1 Tnfr1 Gene ID: 21937 Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: Tnfrsf1b Tnfr-2 Tnfr2 Gene ID: 21938 Accession #: P25119 ELISA Kit: RayBio Custom Mouse sTNFRII ELISA	TNF alpha Gene Symbol: Tnf Tnfa Tnfsf2 Gene ID: 21926 Accession #: P06804 ELISA Kit: RayBio Mouse TNF-alpha ELISA
TNF beta (TNFSF1B) Gene Symbol: Lta Tnfb Tnfsf1 Gene ID: 16992 Accession #: P09225	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: Tnfrsf10b Dr5 Killer Gene ID: Tnfrsf10b Dr5 Killer Accession #: Q9QZM4 ELISA Kit: RayBio Custom Mouse TRAIL R2/TNFRSF10B ELISA	XIAP Gene Symbol: Xiap Aipa Api3 Birc4 Miha Gene ID: 11798 Accession #: Q60989 ELISA Kit: RayBio Custom Mouse XIAP ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Apoptosis Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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