Pathway:
- Akt Signaling
- IGF Signaling
- MAPK Signaling
- NFkB Signaling
- p53 Signaling
- PI3K-AKT Signaling
Number of Targets Detected:
38
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Size:
2, 4, or 8 Sample Kit
Gene Symbols:
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | CASP3 | CASP8
| CD40 | CD40LG | CDKN1A | DIABLO | FAS | FASLG | HSPA4 | HSPB1 | HSPD1 | HTRA2 | IGF1 | IGF2 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IGFBP5 | IGFBP6 | LTA | PER27 | TNF | TNFRSF10B | TNFRSF1A | TNFRSF1B | TNFRSF21 | TP53 | XIAP  View more  Hide
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Scroll over each target protein for more information |
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bax
- Gene Symbol: Bax
- Gene ID: 12028
- Accession #: Q07813
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BIM
- Gene Symbol: Bcl2l11 Bim
- Gene ID: 12125
- Accession #: O54918
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Caspase-3
- Gene Symbol: Casp3 Cpp32
- Gene ID: 12367
- Accession #: P70677
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Caspase-8
- Gene Symbol: Casp8
- Gene ID: 12370
- Accession #: O89110
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HSP60
- Gene Symbol: Hspd1 Hsp60
- Gene ID: 15510
- Accession #: P63038
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HTRA2
- Gene Symbol: Htra2 Omi Prss25
- Gene ID: 64704
- Accession #: Q9JIY5
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p21
- Gene Symbol: Cdkn1a Cip1 Waf1
- Gene ID: 12575
- Accession #: P39689
- ELISA Kit: RayBio
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p53
- Gene Symbol: Tp53 P53 Trp53
- Gene ID: 22059
- Accession #: P02340
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Survivin (BIRC5)
- Gene Symbol: Birc5 Api4 Iap4
- Gene ID: 11799
- Accession #: O70201
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TNF beta (TNFSF1B)
- Gene Symbol: Lta Tnfb Tnfsf1
- Gene ID: 16992
- Accession #: P09225
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Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.