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Mouse Apoptosis Array C1

RayBio® C-Series Mouse Apoptosis Antibody Array 1 Kit. Detects 38 Mouse Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$365.62
AAM-APO-1-2
+

Antigen Information

Gene Symbols:
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | CASP3 | CASP8   View more

Assay Format

Pathway:
  • Akt Signaling
  • IGF Signaling
  • MAPK Signaling
  • NFkB Signaling
  • p53 Signaling
  • PI3K-AKT Signaling
Number of Targets Detected:
38
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad
bax
  • Gene Symbol: Bax
  • Gene ID: 12028
  • Accession #: Q07813
bcl-2
bcl-w
BID
BIM
  • Gene Symbol: Bcl2l11 Bim
  • Gene ID: 12125
  • Accession #: O54918
Caspase-3
  • Gene Symbol: Casp3 Cpp32
  • Gene ID: 12367
  • Accession #: P70677
Caspase-8
  • Gene Symbol: Casp8
  • Gene ID: 12370
  • Accession #: O89110
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
cIAP-2
Cytochrome C
 
DR6
(TNFRSF21)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
HSP27
HSP60
  • Gene Symbol: Hspd1 Hsp60
  • Gene ID: 15510
  • Accession #: P63038
HSP70
HTRA2
  • Gene Symbol: Htra2 Omi Prss25
  • Gene ID: 64704
  • Accession #: Q9JIY5
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-5
IGFBP-6
IGF-1
IGF-2
p21
  • Gene Symbol: Cdkn1a Cip1 Waf1
  • Gene ID: 12575
  • Accession #: P39689
  • ELISA Kit: RayBio
p27/Kip1
(CDKN1B)
p53
  • Gene Symbol: Tp53 P53 Trp53
  • Gene ID: 22059
  • Accession #: P02340
SMAC
Survivin
(BIRC5)
  • Gene Symbol: Birc5 Api4 Iap4
  • Gene ID: 11799
  • Accession #: O70201
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TNF alpha
TNF beta
(TNFSF1B)
  • Gene Symbol: Lta Tnfb Tnfsf1
  • Gene ID: 16992
  • Accession #: P09225
TRAIL R2
(TNFRSF10B/DR5)
XIAP
   

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department ([email protected]).
Total list price $481.77
Price for all $400.46

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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