Mouse bFGF ELISA

RayBio® Mouse bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELM-bFGF-1
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Assay Format

Detection Range:
8 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
8 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P15655
Gene ID:
  • 14173
Gene Symbols:
  • FGF-2
  • FGF2
Protein Name & Synonyms:
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kim, Bae Jin, et al. "Synergistic vasculogenic effects of AMD3100 and stromal-cell-derived factor-1? in vasa nervorum of the sciatic nerve of mice with diabetic peripheral neuropathy." Cell and tissue research 354.2 (2013): 395-407.
    Species: Mouse
    Sample type: Tissue Lysate (sciatic nerve - diabetic peripheral neuropathy model)
  2. Kim, Bae Jin, et al. "Synergistic vasculogenic effects of AMD3100 and stromal-cell-derived factor-1? in vasa nervorum of the sciatic nerve of mice with diabetic peripheral neuropathy." Cell and tissue research 354.2 (2013): 395-407.
    Species: Mouse
    Sample type: Tissue Lysate (left hind limb muscles - diabetic peripheral neuropathy model)
  3. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample type: Tissue Lysate (Skeletal muscle lysate from plantaris muscle)
  4. Tsubaki M., et al. Blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathways by statins reduces the expression of bFGF, HGF, and TGF-? as angiogenic factors in mouse osteosarcoma. Cytokine. 2011 Apr;54(1):100-7. doi: 10.1016/j.cyto.2011.01.005
    Species: Mouse
    Sample type:
  5. Talbot N., Sparks W., Powell A., et al. Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):1-11. doi: 10.1007/s11626-011-9467-7
    Species: Mouse
    Sample type: Conditioned Media (Primary CF-1 fibtoblasts from 13.5d fetsus of CF-1 mice, and STO fibroblasts)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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