Mouse Bradykinin EIA

RayBio® Human/Mouse/Rat Bradykinin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.

$397.00  
EIAM-BRAK-1
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Assay Format

Detection Range:
0.1-1,000 ng/ml
Recommended Dilution (serum & plasma):
Sample extraction
Sensitivity:
1.4 ng/ml
Specificity:
This EIA kit is designed to detect human, mouse, and rat Dynorphin
Species Detected:
  • Human
  • Mouse
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Competition-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • O08677
Gene ID:
  • 16644
Gene Symbols:
  • KNG
  • KNG1
Protein Name & Synonyms:
Kininogen-1 (Alpha-2-thiol proteinase inhibitor) (Fitzgerald factor) (High molecular weight kininogen) (HMWK) (Williams-Fitzgerald-Flaujeac factor) [Cleaved into: Kininogen-1 heavy chain T-kinin (Ile-Ser-Bradykinin) Bradykinin (Kallidin I) Lysyl-bradykinin (Kallidin II) Kininogen-1 light chain Low molecular weight growth-promoting factor]

Introduction

Bradykinin is a nine amino acids peptide that is cleaved from kininogen precursor. It has a variety of physiological effects: 1) it causes blood vessels to dilate, and leads to a drop in blood pressure; 2) influence in smooth muscle contraction; 3) natriuresis and diuresis; 4) decrease in blood glucose level; 5) it is a mediator of inflammation and causes increase in vascular permeability, stimulation of nociceptors, release of other mediators of inflammation (e.g. prostaglandins); 6) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Typical Data

Standard Curve

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 µl detection antibody to each well.
  3. Incubate 1.5 h at RT or O/N at 4°C.
  4. Add 100 µl standard or sample to each well.
  5. Incubate 2.5 h at RT.
  6. Add 100 µl prepared streptavidin solution.
  7. Incubate 45 min at RT.
  8. Add 100 µl TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl Stop Solution to each well.
  11. Read plate at 450 nm immediately.

Storage/Stability

Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
The kit is easy to use! Great!! All buffers in the kit is more enough to analyze.

University of Rochester
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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