Mouse Chemokine Array C1

RayBio® C-Series Mouse Chemokine Antibody Array 1 Kit. Detects 25 Mouse Chemokines. Suitable for all liquid sample types.

$944.35  
AAM-CHE-1-8
+

Assay Format

Number of Targets Detected:
25
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL21 | CCL22 | CCL24   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
BLC
(CXCL13)
CTACK
(CCL27)
CXCL16
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fractalkine
(CX3CL1)
I-TAC
(CXCL11)
KC
(CXCL1)
LIX
MCP-1
(CCL2)
MCP-5
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
Platelet Factor 4
(CXCL4)
RANTES
(CCL5)
SDF-1
(CXCL12)
  • Gene Symbol: CXCL12
  • Accession #: P40224
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang, Xiu-Xing, et al. "Altered protein prenylation in Sertoli cells is associated with adult infertility resulting from childhood mumps infection." Journal of Experimental Medicine 210.8 (2013): 1559-1574.
    Species: Mouse
    Sample type: Conditioned Media ( primary Sertoli cells )
  2. Li, L., Ni, L., Heary, R.F. et al. Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury. J Neuroinflammation 17, 73 (2020). https://doi.org/10.1186/s12974-020-01748-x
    Species: Mouse
    Sample type: Conditioned media (cell culture media)
  3. Birkenfeld, Andreas L., et al. "Influence of the hepatic eukaryotic initiation factor 2? (eIF2?) endoplasmic reticulum (ER) stress response pathway on insulin-mediated ER stress and hepatic and peripheral glucose metabolism." Journal of Biological Chemistry 286.42 (2011): 36163-36170.
    Species: Mouse
    Sample type: Plasma
  4. Swamydas, Muthulekha, et al. "Mesenchymal stem cell-derived CCL-9 and CCL-5 promote mammary tumor cell invasion and the activation of matrix metalloproteinases." Cell adhesion & migration 7.3 (2013): 315-324.
    Species: Mouse
    Sample type: Conditioned Media (Normal mouse mammary gland cells (NMuMG), metastatic mouse mammary cells (4T1), and mesenchymal murine stem (D1) cell)
  5. Jrad-Lamine, Aicha, et al. "Indoleamine 2, 3-dioxygenase 1 (IDO1) is involved in the control of mouse caput epididymis immune environment." PloS one 8.6 (2013): e66494.
    Species: Mouse
    Sample type: Tissue Lysate (caput epididymal protein extracts )
  6. Jrad-Lamine, Aicha, et al. "Indoleamine 2, 3-dioxygenase 1 (IDO1) is involved in the control of mouse caput epididymis immune environment." PloS one 8.6 (2013): e66494.
    Species: Mouse
    Sample type: Plasma (caput epididymal protein extracts )

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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