Mouse Cytokine Array C1

RayBio® C-Series Mouse Cytokine Antibody Array 1 Kit. Detects 22 Mouse Cytokines. Suitable for all liquid sample types.

$479.35  
AAM-CYT-1-4
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Antigen Information

Gene Symbols:
CCL12 | CCL2 | CCL5 | CSF2 | CSF3 | IFNG | IL10 | IL12A | IL13 | IL17A   View more

Assay Format

Number of Targets Detected:
22
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
IFN-gamma
MCP-1
(CCL2)
MCP-5
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample type: Conditioned Media
  2. Kim B, Kim JE, Choi B-K, Kim H-S. Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-?B-signaling Pathway. Biomolecules & Therapeutics 2015;23(1):90-97. doi:10.4062/biomolther.2014.080.
    Species: Mouse
    Sample type: Conditioned Media
  3. Wisnewski AV., Liu J., Colangelo CM. Glutathione reaction products with a chemical allergen, methylene-diphenyl diisocyanate, stimulate alternative macrophage activation and eosinophilic airway inflammation. Chem Res Toxicol. 2015 Apr 20;28(4):729-37. doi: 10.1021/tx5005002.
    Species: Mouse
    Sample type: BAL Fluid
  4. Yang H., Wang H., Ju Z., et al. MD-2 is required for disulfide HMGB1-dependent TLR4 signaling. J Exp Med. 2015 Jan 12;212(1):5-14. doi: 10.1084/jem.20141318.
    Species: Mouse
    Sample type: Conditioned Media
  5. Collignon A., Perles-Barbacaru A., Robert S., et al. A pancreatic tumor-specific biomarker characterized in humans and mice as an immunogenic onco-glycoprotein is efficient in dendritic cell vaccination. Oncotarget. 2015 Sep 15;6(27):23462-79.
    Species: Mouse
    Sample type: Conditioned Media
  6. Hsu Y, Hsu J., Lin K. Yam storage protein dioscorins modulate cytokine gene expression in BALB/c and C57BL/6 lymphocytes. Food and Agricultural Immunology Volume 26, 2015 - Issue 6, 909-923. http://dx.doi.org/10.1080/09540105.2015.1048787
    Species: Mouse
    Sample type: Conditioned Media
  7. Kobayashi T., Tanaka K., Fujita T., et al. Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis. Respir Res. 2015 Aug 20;16:99. doi: 10.1186/s12931-015-0261-z
    Species: Mouse
    Sample type: Conditioned Media
  8. Hoffmann J., Otte A., Thiele S., et al. Sex differences in H7N9 influenza A virus pathogenesis. Vaccine. 2015 Dec 8;33(49):6949-54. doi: 10.1016/j.vaccine.2015.08.044
    Species: Mouse
    Sample type: Tissue Lysate
  9. Carnevalli L., Scognamiglio R., Cabezas-Wallschied N., Rahmig S., Laurenti E. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. JEM vol. 211 no. 5 769-779
    Species: Mouse
    Sample type: Conditioned Media
  10. Chae M., Peterson TE., Balgeman A., et al. Increasing glioma-associated monocytes leads to increased intratumoral and systemic myeloid-derived suppressor cells in a murine model. Neuro Oncol. 2014 Dec 23. pii: nou343. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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