Mouse Cytokine Array C1

RayBio® C-Series Mouse Cytokine Antibody Array 1 Kit. Detects 22 Mouse Cytokines. Suitable for all liquid sample types.

$479.35  
AAM-CYT-1-4
+

Assay Format

Number of Targets Detected:
22
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL12 | CCL2 | CCL5 | CSF2 | CSF3 | IFNG | IL10 | IL12A | IL13 | IL17A   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
IFN-gamma
MCP-1
(CCL2)
MCP-5
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Nofer J-R., Bot M., Brodde M., et al. FTY720, a synthetic sphingosine 1 phosphate analogue, inhibits development of atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation . 2007 Jan 30;115(4):501-8. doi: 10.1161/CIRCULATIONAHA.106.641407.
    Species: Mouse
    Sample type: Plasma (LDLR defcient mice plasma treated with FTY720)
  2. Sun M., Chen M., Dawood F., et al. Tumor Necrosis Factor-? Mediates Cardiac Remodeling and Ventricular Dysfunction After Pressure Overload State. Circulation . 2007 Mar 20;115(11):1398-407. doi: 10.1161/CIRCULATIONAHA.106.643585.
    Species: Mouse
    Sample type: Tissue Lysate (Cardiac Banding lysate: Cardiac repair and removleding)
  3. Denis Gallagher, Andreea A. Norman, Cameron L. Woodard, et al. "Transient Maternal IL-6 Mediates Long-Lasting Changes in Neural Stem Cell Pools by Deregulating an Endogenous Self-Renewal Pathway." Cell Stem Cell 13, 564-576, November 7, 2013. http://dx.doi.org/10.1016/j.stem.2013.10.002
    Species: Mouse
    Sample type: Conditioned Media (E12.5 and E13.5 embryonic cells)
  4. Loria, Gilbert David, et al. "Platelets support a protective immune response to LCMV by preventing splenic necrosis." Blood 121.6 (2013): 940-950.
    Species: Mouse
    Sample type: Serum (platelet-depleted mice infected with the mouse arenavirus lymphocytic choriomeningitis virus)
  5. Carnevalli L., Scognamiglio R., Cabezas-Wallschied N., Rahmig S., Laurenti E. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. JEM vol. 211 no. 5 769-779
    Species: Mouse
    Sample type: Conditioned Media (Bone Marrow Derived Macrophages cell)
  6. Yang H., Wang H., Ju Z., et al. MD-2 is required for disulfide HMGB1-dependent TLR4 signaling. J Exp Med. 2015 Jan 12;212(1):5-14. doi: 10.1084/jem.20141318.
    Species: Mouse
    Sample type: Conditioned Media (Mouse Macrophages stimulated with HMGB1)
  7. Feng-Chun Y., Ingram D., Chen S., et al. Neurofibromin-deficient Schwann cells secrete a potent migratory stimulus for Nf1+/– mast cells. J Clin Invest. 2003 Dec 15; 112(12): 1851–1861. doi: 10.1172/JCI200319195
    Species: Mouse
    Sample type: Conditioned Media (Schwann cell media cultures)
  8. Timmers L., van Keulen J., Hoefer I., et al. Targeted Deletion of Nuclear Factor ?B p50 Enhances Cardiac Remodeling and Dysfunction Following Myocardial Infarction. Circ Res . 2009 Mar 13;104(5):699-706. doi: 10.1161/CIRCRESAHA.108.189746. Epub 2009 Jan 24.
    Species: Mouse
    Sample type: Conditioned Media (Mouse embryonic fibroblasts simtulated with LPS)
  9. Esteban E., Ruperez M., Sanches-Lopex E., et al. Angiotensin IV Activates the Nuclear Transcription Factor-?B and Related Proinflammatory Genes in Vascular Smooth Muscle Cells. Circ Res . 2005 May 13;96(9):965-73. doi: 10.1161/01.RES.0000166326.91395.74. Epub 2005 Apr 14.
    Species: Mouse
    Sample type: Cell Lysate (Vascular smooth muscle cells with active NFkB)
  10. Pidkovka N., Cherepanova O., Yoshida T., et al. Oxidized Phospholipids Induce Phenotypic Switching of Vascular Smooth Muscle Cells In Vivo and In Vitro. Circ Res . 2007 Oct 12;101(8):792-801.
    Species: Mouse
    Sample type: Conditioned Media (Smooth Muscles Cells treated with osidized phospholipids)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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