Mouse Cytokine Array C2

RayBio® C-Series Mouse Cytokine Antibody Array 2 Kit. Detects 32 Mouse Cytokines. Suitable for all liquid sample types.

$968.79  
AAM-CYT-2-8
+

Assay Format

Number of Targets Detected:
32
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL21 | CCL27 | CCL3 | CCL5   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
CTACK
(CCL27)
Eotaxin-1
(CCL11)
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IFN-gamma
KC
(CXCL1)
Leptin
MCP-1
(CCL2)
MCP-5
MIP-1 alpha
(CCL3)
MIP-2
MIP-3 beta
(CCL19)
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Michel, M., et al. Identification of an IL-17-producing NK1.1neg iNKT cell population involved in airway neutrophilia. Journal of experimental Medicine. 2007, vol. 204 no. 5 995-1001. doi: 10.1084/jem.20061551
    Species: Mouse
    Sample type: Conditioned Media (NK1.1+ sorted cells treated with alphaGalser)
  2. Nakamura, Atsushi, et al. "Transcription repressor Bach2 is required for pulmonary surfactant homeostasis and alveolar macrophage function." Journal of Experimental Medicine(2013): jem-20130028.
    Species: Mouse
    Sample type: BAL fluid
  3. Herrmann, A., Priceman, S., et al. CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells. The Journal of Clinical Investigation 2014. Epub Ahead of Print DOI: 10.1172/JCI73174
    Species: Mouse
    Sample type: Conditioned Media (cell supernatants)
  4. Brown NE, Jeselsohn R, Bihani T, et al. Cyclin D1 activity regulates autophagy and senescence in the mammary epithelium. Cancer research 2012;72(24):6477-6489. doi:10.1158/0008-5472.CAN-11-4139.
    Species: Mouse
    Sample type: Tissue Lysate (Mammary gland built organoid)
  5. Wu, Q et al. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Clin. Cancer Res. Volume 10, Issue 20, Pages 6977-84, Oct 2004
    Species: Mouse
    Sample type: Urine (Mouse Urine Samples)
  6. Rich J, Lee J. The Pathogenesis of Staphylococcus aureus Infection in the Diabetic NOD Mouse. Diabetes. 2005;54:2904-2910.
    Species: Mouse
    Sample type: Tissue Lysate (Mouse hindpaw Lysate)
  7. Landek-Salgado MA, Rose NR, Caturegli P. Placenta Suppresses Experimental Autoimmune Hypophysitis through Soluble TNF Receptor 1. Journal of Autoimmunity 2012;38(2-3):J88-J96. doi:10.1016/j.jaut.2011.07.001.
    Species: Mouse
    Sample type: Tissue Lysate (Placental and Muscle protein extracts)
  8. Le Goffic R, Balloy V, Lagranderie M, Alexopoulou L, et al. Detrimental Contribution of the Toll-Like Receptor 3 (TLR3) to Influenza A Virus-Induced Acute Pneumonia. PLoS Pathog. 2006; 2(6):e53. doi:10.1371/journal.ppat.0020053
    Species: Mouse
    Sample type: BAL Fluid (Bronchoalveolar lavage fluid (BALF))
  9. Chu H, Xiang J, Wu P, et al. The role of aquaporin 4 in apoptosis after intracerebral hemorrhage. Journal of Neuroinflammation 2014;11(1):184. doi:10.1186/s12974-014-0184-5.
    Species: Mouse
    Sample type: Tissue Lysate (Brain Lysates)
  10. Fan, H et al. Cytokine dysregulation induced by apoptotic cells is a shared characteristic of macrophages from nonobese diabetic and systemic lupus erythematosus-prone mice. J. Immunol. Volume 172, Issue 8, Pages 4834-43, Apr 2004
    Species: Mouse
    Sample type: Cell Lysate (M? cell lysates)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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