Mouse Cytokine Array C3

RayBio® C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.

$440.36  
AAM-CYT-3-2
+

Assay Format

Number of Targets Detected:
62
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL24 | CCL25   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Axl
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-9
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
Platelet Factor 4
(CXCL4)
P-Selectin
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VCAM-1
(CD106)
VEGF-A

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Rudilla F., Fayolle C., Casares N., et al. Combination of a TLR4 ligand and anaphylatoxin C5a for the induction of antigen-specific cytotoxic T cell responses. Vaccine. 2012 Apr 16;30(18):2848-58. doi: 10.1016/j.vaccine.2012.02.052.
    Species: Mouse
    Sample type: Conditioned Media (BMDCs stimulated with SIINFEKL and C5a + SIINFEKL)
  2. Ranasinghe, Charani, et al. "A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus–poxvirus prime boost immunisations." Vaccine 29.16 (2011): 3008-3020.
    Species: Mouse
    Sample type: Conditioned Media
  3. Taka E., Mazzio E., Goodman C., et al. Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells. J Neuroimmunol. 2015 Sep 15;286:5-12. doi: 10.1016/j.jneuroim.2015.06.011.
    Species: Mouse
    Sample type: Conditioned Media (BV-2 microglial cells, treated with thymoquinone)
  4. Maeda, Shin, et al. "Colon cancer-derived factors activate NF-kappaB in myeloid cells via TLR2 to link inflammation and tumorigenesis." Mol Med Rep 4.6 (2011): 1083-1088.
    Species: Mouse
    Sample type: Conditioned Media (BMDMs)
  5. Ha CT, Li X-H, Fu D, Moroni M, Fisher C, et al. (2014) Circulating Interleukin-18 as a Biomarker of Total-Body Radiation Exposure in Mice, Minipigs, and Nonhuman Primates (NHP). PLoS ONE 9(10): e109249. doi:10.1371/journal.pone.0109249
    Species: Mouse
    Sample type: Serum
  6. Kotas, Maya E., et al. "Impact of CD1d deficiency on metabolism." PLoS One 6.9 (2011): e25478.
    Species: Mouse
    Sample type: Tissue Lysate (obese mice relative to lean control livers)
  7. Huynh, Duy, et al. "CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response." PLoS One 8.2 (2013): e56951.
    Species: Mouse
    Sample type: Tissue Lysate (pregnant mouse uterus extract)
  8. Miernikiewicz, Paulina, et al. "T4 phage and its head surface proteins do not stimulate inflammatory mediator production." PLoS One 8.8 (2013): e71036.
    Species: Mouse
    Sample type: Plasma
  9. Lawrence DW, Shornick LP, Kornbluth J (2019) Mice deficient in NKLAM have attenuated inflammatory cytokine production in a Sendai virus pneumonia model. PLoS ONE 14(9): e0222802. https://doi.org/10.1371/journal.pone.0222802
    Species: Mouse
    Sample type: Tissue Lysate (Lung)
  10. Chen YF, Wang YH, Lei CS, Changou CA, Davis ME, Yen Y. Host immune response to anti-cancer camptothecin conjugated cyclodextrin-based polymers. J Biomed Sci. 2019 Oct 23;26(1):85. doi: 10.1186/s12929-019-0583-0. PMID: 31647037; PMCID: PMC6806548.
    Species: Mouse
    Sample type: Serum, Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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