Mouse Cytokine Array C3

RayBio® C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.

$617.00  
AAM-CYT-3-4
+

Assay Format

Number of Targets Detected:
62
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL24 | CCL25   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Axl
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-9
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
Platelet Factor 4
(CXCL4)
P-Selectin
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VCAM-1
(CD106)
VEGF-A

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Ma C, Witkowski MT, Harris J, Dolgalev I, Sreeram S, Qian W, Tong J, Chen X, Aifantis I, Chen W. Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche. Sci Adv. 2020 Oct 30;6(44):eaba5536. doi: 10.1126/sciadv.aba5536. PMID: 33127669; PMCID: PMC7608809.
    Species: Mouse
    Sample type: Conditioned Media
  2. Choudhary M, Ismail EN, Yao PL, Tayyari F, Radu RA, Nusinowitz S, Boulton ME, Apte RS, Ruberti JW, Handa JT, Tontonoz P, Malek G. LXRs regulate features of age-related macular degeneration and may be a potential therapeutic target. JCI Insight. 2020 Jan 16;5(1):e131928. doi: 10.1172/jci.insight.131928. PMID: 31829999; PMCID: PMC7030875.
    Species: mouse
    Sample type: Tissue Lysate
  3. Chen, YF., Wang, YH., Lei, CS. et al. Host immune response to anti-cancer camptothecin conjugated cyclodextrin-based polymers. J Biomed Sci 26, 85 (2019). https://doi.org/10.1186/s12929-019-0583-0
    Species: Mouse
    Sample type: Serum
  4. Lawrence DW, Shornick LP, Kornbluth J (2019) Mice deficient in NKLAM have attenuated inflammatory cytokine production in a Sendai virus pneumonia model. PLoS ONE 14(9): e0222802. https://doi.org/10.1371/journal.pone.0222802
    Species: Mouse
    Sample type: Tissue Lysate
  5. Chen YF, Wang YH, Lei CS, Changou CA, Davis ME, Yen Y. Host immune response to anti-cancer camptothecin conjugated cyclodextrin-based polymers. J Biomed Sci. 2019 Oct 23;26(1):85. doi: 10.1186/s12929-019-0583-0. PMID: 31647037; PMCID: PMC6806548.
    Species: Mouse
    Sample type: Serum, Plasma
  6. Simon M., Ven Meter M., Ablaeva J., et al. LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation. Cell Metab . 2019 Apr 2;29(4):871-885.e5. doi: 10.1016/j.cmet.2019.02.014. Epub 2019 Mar 7.
    Species: Mouse
    Sample type: Plasma
  7. Himburg H., Doan P., Quarmyne M., et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. doi: 10.1038/nm.4251.
    Species: Mouse
    Sample type: Bone Marrow Aspirate
  8. Li J., Zhou Z., Zhang X., et al. Inflammatory Molecule, PSGL-1, Deficiency Activates Macrophages to Promote Colorectal Cancer Growth through NF?B Signaling. Mol Cancer Res. 2017 Apr;15(4):467-477. doi: 10.1158/1541-7786.MCR-16-0309.
    Species: Mouse
    Sample type: Serum
  9. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media
  10. Moon H., Donahue L., Choi E., et al. Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure. Cell Stem Cell . 2017 Nov 2;21(5):665-678.e6. doi: 10.1016/j.stem.2017.09.001. Epub 2017 Oct 12.
    Species: Mouse
    Sample type: Conditioned Media

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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