Mouse Cytokine Array C4

RayBio® C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL22 | CXCL11 | CXCL15 | DPP4 | FCGR2B | FGF2 | FIGF | FLT1 | FLT3LG | FLT4   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Fc gamma RIIB
Flt-3 Ligand
IL-17 RB
Sonic Hedgehog N-Terminal
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Cyclophilin D Extramitochondrial Signaling Controls Cell Cycle Progression and Chemokine-Directed Cell Motility
    Species: Mouse
    Sample type: Conditioned Media (embryonic fibroblast cells, mutant vs. wild-type)
  2. Haldar S., Dru C., Choudhury D., et al. Inflammation and pyroptosis mediate muscle expansion in an IL-1? -dependent manner. The Journal of Biological Chemistry 290, 6574-6583. doi: 10.1074/jbc.M114.617886
    Species: Mouse
    Sample type: Tissue Lysate (Bladder tissue lysates in a muscle cell dath model)
  3. Jin H., Chen L., Zhang Z., et al. Deletion of angiotensin-converting enzyme 2 exacerbates renal inflammation and injury in apolipoprotein E-deficient mice through modulation of the nephrin and TNF-alpha-TNFRSF1A signaling. J Transl Med. 2015 Aug 6;13:255. doi: 10.1186/s12967-015-0616-8.
    Species: Mouse
    Sample type: Tissue Lysate (Renal lysate samples from Ace2 deficient mice)
  4. Pavlides S., et al. Caveolin-1 regulates the anti-atherogenic properties of macrophages. Cell Tissue Res (2014) 358:821-831. DOI 10.1007/s00441-014-2008-4
    Species: Mouse
    Sample type: Conditioned Media (Peritoneal Macrophages LPS treated)
  5. Fang, Wei Bin, et al. "Loss of one Tgfbr2 allele in fibroblasts promotes metastasis in MMTV: polyoma middle T transgenic and transplant mouse models of mammary tumor progression." Clinical & experimental metastasis 28.4 (2011): 351-366.
    Species: Mouse
    Sample type: Conditioned Media
  6. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample type: Conditioned Media (Premetastatic PyV MT mice lungs)
  7. Taka E., Mazzio E., Goodman C., et al. Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells. J Neuroimmunol. 2015 Sep 15;286:5-12. doi: 10.1016/j.jneuroim.2015.06.011.
    Species: Mouse
    Sample type: Conditioned Media (BV-2 microglial cells, treated with thymoquinone)
  8. Arshad A, Deutsch E, Vozenin M-C (2015) Simultaneous Irradiation of Fibroblasts and Carcinoma Cells Repress the Secretion of Soluble Factors Able to Stimulate Carcinoma Cell Migration. PLoS ONE 10(1): e0115447. doi:10.1371/journal. pone.0115447
    Species: Mouse
    Sample type: Conditioned Media (TC-1 cells in a wound healing assay)
  9. Chen YF, Wang YH, Lei CS, Changou CA, Davis ME, Yen Y. Host immune response to anti-cancer camptothecin conjugated cyclodextrin-based polymers. J Biomed Sci. 2019 Oct 23;26(1):85. doi: 10.1186/s12929-019-0583-0. PMID: 31647037; PMCID: PMC6806548.
    Species: Mouse
    Sample type: Serum, Plasma

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