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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/Cytokine Arrays/Mouse Cytokine Array C4

Manual / SDS

Description
Citations
Reviews
Files

Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Accession #: P28843 ELISA Kit: RayBio Mouse DPPIV ELISA	Dtk Gene Symbol: TYRO3 Accession #: O09080 ELISA Kit: RayBio Mouse Dtk ELISA	E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA	Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Accession #: P08101 ELISA Kit: RayBio Mouse Fc-gamma-RIIb ELISA
Flt-3 Ligand Gene Symbol: FLT3LG Accession #: P49772 ELISA Kit: RayBio Mouse Flt-3L ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA	HGFR Gene Symbol: MET Accession #: D3YVY2 ELISA Kit: RayBio Mouse HGF R ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Accession #: P13597 ELISA Kit: RayBio Mouse ICAM-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA
IGF-1 Gene Symbol: IGF1 Accession #: P05018 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGF-2 Gene Symbol: Igf2 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17 RB Gene Symbol: IL17RB Accession #: Q9JIP3 ELISA Kit: RayBio Mouse IL-17B R ELISA	IL-7 Gene Symbol: IL7 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA
I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	Lungkine (CXCL15) Gene Symbol: CXCL15 Accession #: Q3UQ15 ELISA Kit: RayBio Mouse CXCL15 ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MMP-2 Gene Symbol: MMP2 Accession #: P33434 ELISA Kit: RayBio Mouse MMP-2 ELISA	MMP-3 Gene Symbol: MMP3 Accession #: P28862 ELISA Kit: RayBio Mouse MMP-3 ELISA
Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA	Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	Resistin Gene Symbol: RETN Accession #: Q99P87 ELISA Kit: RayBio Mouse Resistin ELISA	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH ELISA Kit: RayBio Mouse ShhN ELISA
TCK-1 (CXCL7) Gene Symbol: Ppbp Accession #: Q9EQI5 ELISA Kit: RayBio Mouse TCK-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Accession #: Q6GXA8 ELISA Kit: RayBio Mouse TIMP-2 ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Accession #: Q9R1Y0 ELISA Kit: RayBio Mouse TRANCE ELISA	TROY (TNFRSF19) Gene Symbol: TNFRSF19 Accession #: Q80T13 ELISA Kit: RayBio Mouse TROY ELISA	TSLP Gene Symbol: TSLP Accession #: Q9JIE6 ELISA Kit: RayBio Mouse TSLP ELISA
VEGFR1 Gene Symbol: Flt1 Accession #: Q61517 ELISA Kit: RayBio Mouse VEGF R1 ELISA	VEGFR2 Gene Symbol: KDR Accession #: Q3UQZ6 ELISA Kit: RayBio Mouse VEGFR-2 ELISA	VEGFR3 Gene Symbol: FLT4 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA	VEGF-D Gene Symbol: FIGF Accession #: A2AIH3

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Cytokine Antibody Array C4 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.