Mouse Cytokine Array C4

RayBio® C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.

$583.98  
AAM-CYT-4-4
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Assay Format

Number of Targets Detected:
34
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL22 | CXCL11 | CXCL15 | DPP4 | FCGR2B | FGF2 | FIGF | FLT1 | FLT3LG | FLT4   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bFGF
CD26
(DPPIV)
Dtk
E-Selectin
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
GITR
(TNFRSF18)
HGFR
ICAM-1
(CD54)
IGFBP-2
IGF-1
IGF-2
IL-15
IL-17 RB
IL-7
I-TAC
(CXCL11)
Lungkine
(CXCL15)
MDC
(CCL22)
MMP-2
MMP-3
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pro-MMP-9
Resistin
Sonic Hedgehog N-Terminal
(Shh-N)
TCK-1
(CXCL7)
TIMP-2
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TSLP
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media (Stroma derived cytokines)
  2. Himburg H., Doan P., Quarmyne M., et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. doi: 10.1038/nm.4251.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (Bone marrow supernates)
  3. Kashiwagi M., Hosoi J., Lai J., et al. Direct control of regulatory T cells by keratinocytes. Nat Immunol. 2017 Mar;18(3):334-343. doi: 10.1038/ni.3661.
    Species: Mouse
    Sample type: Conditioned Media (Primary cultures keratinocytes)
  4. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample type: Tissue Lysate (Mammary tumor lysates)
  5. Braig M., Pallman N., Preukschas M., Steinemann D., Hofmann W., et al. A ‘telomere-associated secretory phenotype’ cooperates with BCR-ABL to drive malignant proliferation of leukemic cells. 2014 Leukemia, Pubslihed April 1, Epub Ahead of Print, doi: 10.1038/leu.2014.95
    Species: Mouse
    Sample type: Conditioned Media (Chronic Myeloid Leukemia cells)
  6. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample type: Conditioned Media (Scr, 4T1 cells)
  7. Pequeuz C., Raymond-Letron I., Blacher S., et al. Stromal Estrogen Receptor-a Promotes Tumor Growth by Normalizing an Increased Angiogenesis. Cancer Res. 2012 Jun 15;72(12):3010-9. doi: 10.1158/0008-5472.CAN-11-3768
    Species: Mouse
    Sample type: Tissue Lysate (B16K1 Tumor Lysate, melanoma)
  8. Yang Y., Gorzelanny C., Bauer AT., et al. Nuclear heparanase-1 activity suppresses melanoma progression via its DNA-binding affinity. Oncogene. 2015 Mar 9. doi: 10.1038/onc.2015.40.
    Species: Mouse
    Sample type: Tissue Lysate (Melonoma Tissue lysates)
  9. Meng X., Vander Ark A., Lee P., et al. Myeloid-specific TGF-? signaling in bone promotes basic-FGF and breast cancer bone metastasis. Oncogene. 2016 May 5;35(18):2370-8. doi: 10.1038/onc.2015.297.
    Species: Mouse
    Sample type: Tissue Lysate (Mouse Tibiae lysates)
  10. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media (Mouse embryonic Fibroblasts)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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