Mouse Cytokine Array C4

RayBio® C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.

$583.98  
AAM-CYT-4-4
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Assay Format

Number of Targets Detected:
34
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL22 | CXCL11 | CXCL15 | DPP4 | FCGR2B | FGF2 | FIGF | FLT1 | FLT3LG | FLT4   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bFGF
CD26
(DPPIV)
Dtk
E-Selectin
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
GITR
(TNFRSF18)
HGFR
ICAM-1
(CD54)
IGFBP-2
IGF-1
IGF-2
IL-15
IL-17 RB
IL-7
I-TAC
(CXCL11)
Lungkine
(CXCL15)
MDC
(CCL22)
MMP-2
MMP-3
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pro-MMP-9
Resistin
Sonic Hedgehog N-Terminal
(Shh-N)
TCK-1
(CXCL7)
TIMP-2
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TSLP
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Achyut B., Shankar A., Iskander A., et al. Bone marrow derived myeloid cells orchestrate antiangiogenic resistance in glioblastoma through coordinated molecular networks. Cancer Lett. 2015 Dec 28;369(2):416-26. doi: 10.1016/j.canlet.2015.09.004.
    Species: Mouse
    Sample type: Tissue Lysate (Gliobastoma tumors)
  2. Hosaka K., Rojas K., Fazal H., et al. Monocyte Chemotactic Protein-1–Interleukin-6–Osteopontin Pathway of Intra-Aneurysmal Tissue Healing. Stroke. 2017 Apr;48(4):1052-1060. doi: 10.1161/STROKEAHA.116.015590
    Species: Mouse
    Sample type: Tissue Lysate (Mouse Aneurysm samples)
  3. Osuka S., Sampetrean O., Shimizu T., et al. IGF1 Receptor Signaling Regulates Adaptive Radioprotection in Glioma Stem Cells.Stem Cells. 2013 Apr;31(4):627-40. doi: 10.1002/stem.132
    Species: Mouse
    Sample type: Conditioned Media (tumor sphere )
  4. Roth W., Kumar V., Beer HD., et al. Keratin 1 maintains skin integrity and participates in an inflammatory network in skin through interleukin-18. J Cell Sci. 2012 Nov 15;125(Pt 22):5269-79. doi: 10.1242/jcs.116574
    Species: Mouse
    Sample type: Cell Lysate (Keratinocytes)
  5. Roth W., Kumar V., Beer HD., et al. Keratin 1 maintains skin integrity and participates in an inflammatory network in skin through interleukin-18. J Cell Sci. 2012 Nov 15;125(Pt 22):5269-79. doi: 10.1242/jcs.116574
    Species: Mouse
    Sample type: Conditioned Media (Keratinocytes)
  6. Kuraitis, Drew, et al. "Ex vivo generation of a highly potent population of circulating angiogenic cells using a collagen matrix." Journal of molecular and cellular cardiology 51.2 (2011): 187-197.
    Species: Mouse
    Sample type: Tissue lysate
  7. Iwamaru, Yoshifumi, et al. "Prion replication elicits cytopathic changes in differentiated neurosphere cultures." Journal of virology 87.15 (2013): 8745-8755.
    Species: Mouse
    Sample type: Cell Lysate (dNP0 and dNP20 cultures (Differentiated Neurosphere Cultures))
  8. Iwamaru, Yoshifumi, et al. "Prion replication elicits cytopathic changes in differentiated neurosphere cultures." Journal of virology 87.15 (2013): 8745-8755.
    Species: Mouse
    Sample type: Conditioned Media (dNP0 and dNP20 cultures (Differentiated Neurosphere Cultures))
  9. Ivshina M., Alexandrov IM., Vertii A., et al. CPEB Regulation of TAK1 Synthesis Mediates Cytokine Production and the Inflammatory Immune Response. Mol Cell Biol. 2015 Feb 1;35(3):610-8. doi: 10.1128/MCB.00800-14
    Species: Mouse
    Sample type: Serum (Control and CPEB KO mice)
  10. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014; Published OnlineFirst July 24, 2014; doi: 10.1158/1541-7786.MCR-14-0056-T
    Species: Mouse
    Sample type: Conditioned Media (Ba/F3 and ETV6-RUNX1 cell lines)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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