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Home/Search results/Products/Arrays/Antibody Arrays/C-Series: Membrane-Based Arrays/Cytokine Arrays/Mouse Cytokine Array C5

Manual / SDS

Description
Citations
Reviews
Files

Antigen Information

Gene Symbols:

ACE | AGER | AREG | CCL21 | CD27 | CD36 | CD40LG | CD70 | CDH1 | CHRD View more

Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Accession #: P20334 ELISA Kit: RayBio Mouse 4-1BB ELISA	6Ckine (CCL21) Gene Symbol: CCL21 Accession #: P86792 ELISA Kit: RayBio Mouse 6Ckine ELISA	ACE Gene Symbol: ACE Accession #: P22967 ELISA Kit: RayBio Mouse ACE ELISA	ALK-1 Gene Symbol: SLPI Accession #: O09081 ELISA Kit: RayBio Mouse ALK-1 ELISA	Amphiregulin Gene Symbol: AREG Accession #: P31955 ELISA Kit: RayBio Mouse AREG ELISA
Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Accession #: Q60753 ELISA Kit: RayBio Mouse CT-1 ELISA	CD27 (TNFRSF7) Gene Symbol: CD27 Accession #: P41272 ELISA Kit: RayBio Mouse CD27 ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 Accession #: P32970 ELISA Kit: RayBio Mouse CD27 L ELISA	CD36 (SR-B3) Gene Symbol: CD36 Accession #: Q08857 ELISA Kit: RayBio Mouse CD36 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Accession #: P27548 ELISA Kit: RayBio Mouse CD40 L ELISA
Chordin Gene Symbol: CHRD Accession #: Q9Z0E2 ELISA Kit: RayBio Mouse Chordin ELISA	CTLA-4 (CD152) Gene Symbol: Ctla4 Accession #: Q9QZZ7 ELISA Kit: RayBio Mouse CTLA-4 ELISA	Decorin Gene Symbol: DCN Accession #: P28654 ELISA Kit: RayBio Mouse Decorin ELISA	DKK-1 Gene Symbol: DKK1 Accession #: Q80UL5 ELISA Kit: RayBio Mouse Dkk-1 ELISA	E-Cadherin Gene Symbol: CDH1 Accession #: P09803 ELISA Kit: RayBio Mouse E-Cadherin ELISA
EGF Gene Symbol: EGF Accession #: E9QNX6 ELISA Kit: RayBio Mouse EGF ELISA	Endoglin (CD105) Gene Symbol: ENG Accession #: Q63961 ELISA Kit: RayBio Mouse Endoglin ELISA	Epigen Gene Symbol: EPGN Accession #: Q8CEX5 ELISA Kit: RayBio Mouse Epigen ELISA	Epiregulin Gene Symbol: EREG Accession #: Q61521 ELISA Kit: RayBio Mouse Epiregulin ELISA	Galectin-1 Gene Symbol: LGALS1 Accession #: P17601 ELISA Kit: RayBio Mouse Galectin-1 ELISA
Gas 1 Gene Symbol: GAS1 Accession #: Q01721 ELISA Kit: RayBio Mouse GAS1 ELISA	Gas 6 Gene Symbol: Gas6 Accession #: Q99K57 ELISA Kit: RayBio Mouse GAS6 ELISA	GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Accession #: Q7TS55 ELISA Kit: RayBio Mouse GITR L ELISA	Granzyme B Gene Symbol: GZMB Accession #: Q3V2P1 ELISA Kit: RayBio Mouse Granzyme B ELISA	HAI-1 Gene Symbol: SPINT1 Accession #: Q9R097 ELISA Kit: RayBio Mouse HAI-1 ELISA
HGF Gene Symbol: HGF Accession #: Q53WS5 ELISA Kit: RayBio Mouse HGF ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Accession #: Q05208	IL-11 Gene Symbol: IL11 Accession #: P47873 ELISA Kit: RayBio Mouse IL-11 ELISA	IL-17B Gene Symbol: IL17B Accession #: Q99MY3 ELISA Kit: RayBio Mouse IL-17B ELISA	IL-17E (IL-25) Gene Symbol: IL25 Accession #: Q8VHH8 ELISA Kit: RayBio Mouse IL-17E ELISA
IL-17F Gene Symbol: IL17F Accession #: Q7TNI7 ELISA Kit: RayBio Mouse IL-17F ELISA	IL-1 RA (IL-1 F3) Gene Symbol: IL1RN Accession #: P25085 ELISA Kit: RayBio Mouse IL-1 RA ELISA	IL-2 R alpha Gene Symbol: IL2RA Accession #: Q61731 ELISA Kit: RayBio Mouse IL-2 R alpha ELISA	IL-20 Gene Symbol: IL20 Accession #: Q9JKV9 ELISA Kit: RayBio Mouse IL-20 ELISA	IL-21 Gene Symbol: IL21 Accession #: E9PX58 ELISA Kit: RayBio Mouse IL-21 ELISA
IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Accession #: Q4VK74 ELISA Kit: RayBio Mouse IL-28 ELISA	IL-6 R Gene Symbol: IL6R Accession #: P22272 ELISA Kit: RayBio Mouse IL-6 R ELISA	JAM-A (CD321/F11R) Gene Symbol: F11R Accession #: Q8VC39 ELISA Kit: RayBio Mouse JAM-A ELISA	MAdCAM-1 Gene Symbol: MADCAM1 Accession #: Q61278 ELISA Kit: RayBio Mouse MAdCAM-1 ELISA	MFG-E8 Gene Symbol: Mfge8 Accession #: Q9R1X9 ELISA Kit: RayBio Mouse MFG-E8 ELISA
Neprilysin Gene Symbol: MME Accession #: Q6NXX5 ELISA Kit: RayBio Mouse Neprilysin ELISA	Pentraxin-3 (TSG-14) Gene Symbol: PTX3 Accession #: P48759 ELISA Kit: RayBio Mouse TSG-14 ELISA	Prolactin Gene Symbol: PRL Accession #: P06879 ELISA Kit: RayBio Mouse Prolactin ELISA	RAGE Gene Symbol: MOK Accession #: Q9WVS4 ELISA Kit: RayBio Mouse RAGE ELISA	TACI (TNFRSF13B) Gene Symbol: TNFRSF13B Accession #: Q9DBZ3 ELISA Kit: RayBio Mouse TACI ELISA
TREM-1 Gene Symbol: TREM1 Accession #: Q3TAL7 ELISA Kit: RayBio Mouse TREM-1 ELISA	TWEAK (TNFSF12) Gene Symbol: TNFSF12 Accession #: O54907 ELISA Kit: RayBio Mouse TWEAK ELISA	TWEAK R (TNFRSF12) Gene Symbol: TNFRSF12A Accession #: Q9CR75 ELISA Kit: RayBio Mouse TWEAK R ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Cytokine Antibody Array C5 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.