Number of Targets Detected:
48
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Size:
2, 4, or 8 Sample Kit
Gene Symbols:
ACE | AGER | AREG | CCL21 | CD27 | CD36 | CD40LG | CD70 | CDH1 | CHRD
| CTF1 | CTLA4 | DCN | DKK1 | EGF | ENG | EPGN | EREG | F11R | GAS1 | GAS6 | GZMB | HGF | IFNL2 | IL11 | IL17B | IL17F | IL1RL1 | IL1RN | IL20 | IL21 | IL25 | IL2RA | IL6R | LGALS1 | MADCAM1 | MFGE8 | MME | PRL | PTX3 | SLPI | SPINT1 | TNFRSF13B | TNFRSF9 | TNFSF12 | TNFSF18 | TNFSF9 | TREM1  View more  Hide
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Scroll over each target protein for more information |
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| IL-1 R4 (ST2)- Gene Symbol: IL1RL1
- Accession #: Q05208
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Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.