Mouse Cytokine Array C5

RayBio® C-Series Mouse Cytokine Antibody Array 5 Kit. Detects 48 Mouse Cytokines. Suitable for all liquid sample types.

$964.35  
AAM-CYT-5-8
+

Assay Format

Number of Targets Detected:
48
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ACE | AGER | AREG | CCL21 | CD27 | CD36 | CD40LG | CD70 | CDH1 | CHRD   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD36
(SR-B3)
CD40 Ligand
(TNFSF5)
Chordin
CTLA-4
(CD152)
Decorin
DKK-1
E-Cadherin
EGF
Endoglin
(CD105)
Epigen
Epiregulin
Galectin-1
Gas 1
Gas 6
GITR Ligand
(TNFSF18)
Granzyme B
HAI-1
HGF
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-11
IL-17B
IL-17E
(IL-25)
IL-17F
IL-1 RA
(IL-1 F3)
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-6 R
JAM-A
(CD321/F11R)
MAdCAM-1
MFG-E8
Neprilysin
Pentraxin-3
(TSG-14)
Prolactin
RAGE
TACI
(TNFRSF13B)
TREM-1
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media (Stroma derived cytokines)
  2. Himburg H., Doan P., Quarmyne M., et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. doi: 10.1038/nm.4251.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (Bone marrow supernates)
  3. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample type: Tissue Lysate (Mammary tumor lysates)
  4. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample type: Conditioned Media (Scr, 4T1 cells)
  5. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media (Mouse embryonic Fibroblasts)
  6. Lietman C, Wu B, Lechner S, Shinar A, Sehgal M, Rossomacha E, Datta P, Sharma A, Gandhi R, Kapoor M, Young PP. Inhibition of Wnt/?-catenin signaling ameliorates osteoarthritis in a murine model of experimental osteoarthritis. JCI Insight. 2018 Feb 8;3(3):e96308. doi: 10.1172/jci.insight.96308. PMID: 29415892; PMCID: PMC5821202.
    Species: Mouse
    Sample type: Conditioned media
  7. Schubert, Kathleen, et al. "Thy?1 (CD90) regulates the extravasation of leukocytes during inflammation." European journal of immunology 41.3 (2011): 645-656.
    Species: Mouse
    Sample type: BAL Fluid
  8. Verhoeven, D., Pichichero, M., et al. Divergent Mucosal and Systemic Responses in Children in Response to Acute Otitis Media. Clinical & Experimental Immunology 2014 May Epub Ahead of Print DOI: 10.1111/cei.12389
    Species: Mouse
    Sample type: Nasopharyngeal Fluid (Nasopharyngeal Washes)
  9. Puig KL, Floden AM, Adhikari R, Golovko MY, Combs CK (2012) Amyloid Precursor Protein and Proinflammatory Changes Are Regulated in Brain and Adipose Tissue in a Murine Model of High Fat Diet-Induced Obesity. PLoS ONE 7(1): e30378. doi:10.1371/journal.pone.0030378
    Species: Mouse
    Sample type: Conditioned Media (Peritoneally ellicited macrophages)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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