Mouse Cytokine Array C6

RayBio® C-Series Mouse Cytokine Antibody Array 6 Kit. Detects 97 Mouse Cytokines. Suitable for all liquid sample types.

$910.62  
AAM-CYT-6-4
+

Assay Format

Number of Targets Detected:
97
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AREG | AXL | CCL11 | CCL17 | CCL19 | CCL2 | CCL20 | CCL21 | CCL22 | CCL24   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
ALK-1
Amphiregulin
Axl
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD36
(SR-B3)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
E-Cadherin
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
Gas 6
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
Granzyme B
HAI-1
HGF
IFN-gamma
IGFBP-5
IGFBP-6
IGF-2
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-11
IL-12 p40
  • Gene Symbol: IL12B
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-20
IL-21
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
L-Selectin
(CD62L)
Lungkine
(CXCL15)
MAdCAM-1
MCP-1
(CCL2)
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-2
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Prolactin
Pro-MMP-9
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 4-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Ruckh J., Zhao J., Shadrach J., et al. Rejuvenation of regeneration in the aging central nervous system. Cell Stem Cell. 2012 Jan 6;10(1):96-103. doi: 10.1016/j.stem.2011.11.019.
    Species: Mouse
    Sample type: Serum (Parabiotic mice in CNS aging studies)
  2. Cook, R. et al. MerTK Inhibition in Tumor Leukocytes Decreases Tumor Growth and Metastasis. J Clin Invest. Aug 1, 2013; 123(8): 3231-3242. DOI:10.1172/JCI67655
    Species: Mouse
    Sample type: Tissue Lysate (mPyV Mammary Tumors)
  3. Torres S., et al. Proteome Profiling of Cancer-Associated Fibroblasts Identifies Novel Proinflammatory Signatures and Prognostic Markers for Colorectal Cancer. Clin Cancer Res; 19(21) November 1, 2013. DOI: 10.1158/1078-0432.CCR-13-1130
    Species: Mouse
    Sample type: Conditioned Media
  4. Chaballe, Linda, et al. "Involvement of placental growth factor in Wallerian degeneration." Glia 59.3 (2011): 379-396.
    Species: Mouse
    Sample type: Tissue lysate
  5. Rizzi C, Tiberi A, Giustizieri M, Marrone MC, Gobbo F, Carucci NM, Meli G, Arisi I, D'Onofrio M, Marinelli S, Capsoni S, Cattaneo A. NGF steers microglia toward a neuroprotective phenotype. Glia. 2018 Jul;66(7):1395-1416. doi: 10.1002/glia.23312. Epub 2018 Feb 23. PMID: 29473218; PMCID: PMC6001573.
    Species: Mouse
    Sample type: Cell Lysate (Primary Microglia cells)
  6. Zhang, T., Zhou, J., Man, G. C. W., Leung, K. T., Liang, B., Xiao, B., … Wang, C. C. (2018). MDSCs drive the process of endometriosis by enhancing angiogenesis and are a new potential therapeutic target. European Journal of Immunology, 48(6), 1059–1073. doi:10.1002/eji.201747417
    Species: Mouse
    Sample type: Peritoneal Fluid
  7. Ey, B., Eyking, A., Klepak, M., Salzman, N. H., Göthert, J. R., Rünzi, M., ... & Cario, E. (2013). Loss of TLR2 worsens spontaneous colitis in MDR1A deficiency through commensally induced pyroptosis. The Journal of Immunology, 190(11), 5676-5688.
    Species: Mouse
    Sample type: Tissue Lysate (myeloid cells and colonic tissue samples )
  8. Zhu, Xiao-Dong, et al. "Antiangiogenic therapy promoted metastasis of hepatocellular carcinoma by suppressing host-derived interleukin-12b in mouse models." Angiogenesis 16.4 (2013): 809-820.
    Species: Mouse
    Sample type: Plasma (BalbC mice HCC model)
  9. Guyot, N., Wartelle, J. et al. Unopposed Cathepsin G, Neutrophil Elastase, and Proteinase 3 Cause Severe Lung Damage and Emphysema. The American Journal of Pathology 2014. Epub Ahead of Print. DOI: 10.1016/j.ajpath.2014.04.015
    Species: Mouse
    Sample type: BAL Fluid
  10. Cheng, Kang, et al. "Bone-derived mesenchymal stromal cells from HIV transgenic mice exhibit altered proliferation, differentiation capacity and paracrine functions along with impaired therapeutic potential in kidney injury." Experimental cell research 319.14 (2013): 2266-2274.
    Species: Mouse
    Sample type: Conditioned Media (Tg26 HIV-1 MSCs and FVB/N MSCs )

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Not for diagnostic or therapeutic use.

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