Mouse Cytokine Array C7

RayBio® C-Series Mouse Cytokine Antibody Array 7 Kit. Detects 40 Mouse Cytokines. Suitable for all liquid sample types.

$358.55  
AAM-CYT-7-2
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Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AHSG | BTC | C5 | CCL6 | CD48 | CD6 | CD80 | CDH3 | CLU | CST3   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CD80
(B7-1)
BAFF R
Betacellulin
(BTC)
C5a
CCL6
CD48
(SLAMF2)
CD6
Chemerin
Clusterin
Lungkine
(CXCL15)
Cystatin C
DAN
DLL4
EDAR
Endocan
Fetuin A
H60
IL-33
(IL-1 F11)
IL-7 R alpha
Kremen-1
Limitin
Lipocalin-2
(NGAL)
LOX-1
Marapsin
MBL-2
Meteorin
Nope
NOV
(CCN3)
Osteoactivin
(GPNMB)
  • Gene Symbol: GPNMB
  • Accession #: Q99P91
OX40 Ligand
  • Gene Symbol: TNFSF4
  • Gene ID: 7292
P-Cadherin
Periostin
PLGF-2
Progranulin
Prostasin
Renin 1
Testican 3
(SPOCK3)
TIM-1
(KIM-1)
TRAIL
(TNFSF10)
Tryptase epsilon

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 4-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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