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Antigen Information
Gene Symbols:
CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL21 | CCL27 | CCL3 | CCL5 | CSF2| CSF3 | CXCL1 | CXCL2 | IFNG | IL10 | IL12A | IL13 | IL17A | IL2 | IL3 | IL4 | IL5 | IL6 | IL9 | KITLG | LEP | THPO | TIMP1 | TNF | TNFRSF1A | TPO | VEGFA View more
Assay Format
Number of Targets Detected:
32
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
4 or 8 Sample Kit
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| CTACK (CCL27)
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
|
IL-2
| IL-3
| IL-4
| IL-5
| IL-6
|
IL-9
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-17A
| IFN-gamma
| KC (CXCL1)
| Leptin
| MCP-1 (CCL2)
|
MCP-5
| MIP-1 alpha (CCL3)
| MIP-2
| MIP-3 beta (CCL19)
| RANTES (CCL5)
|
SCF
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TIMP-1
| TNF alpha
|
Thrombopoietin (TPO)
| VEGF-A
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array G2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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Pelosi L., Berardinelli M., De Pasquale L., et al. Functional and Morphological Improvement of Dystrophic Muscle by Interleukin 6 Receptor Blockade. EBioMedicine. 2015 Feb 26;2(4):285-93. doi: 10.1016/j.ebiom.2015.02.014
Species: Mouse
Sample type: Tissue Lysate -
Geoffrion, M., Du, X. et al. Differential effects of glyoxalase 1 overexpression on diabetic atherosclerosis and renal dysfunction in streptozotocin?treated, apolipoprotein E?deficient mice. Physiological Reports 2014. Epub Ahead of Print. DOI: 10.14814/phy2.12043
Species: Mouse
Sample type: Tissue Lysate -
Yang J, Kantrow S, Sai J, et al. INK4a/ARF inactivation with activation of the NF-?B/IL-6 pathway is sufficient to drive the development and growth of angiosarcoma. Cancer research 2012;72(18):4682-4695. doi:10.1158/0008-5472.CAN-12-0440
Species: Mouse
Sample type: Serum -
Lerner, Immanuel, et al. "Heparanase powers a chronic inflammatory circuit that promotes colitis-associated tumorigenesis in mice." The Journal of clinical investigation 121.5 (2011): 1709-1721.
Species: Mouse
Sample type: Conditioned Media -
McDonald, Claire L., et al. "Nogo receptor is involved in the adhesion of dendritic cells to myelin." Journal of neuroinflammation 8.1 (2011): 113.
Species: Mouse
Sample type: Conditioned Media -
Shin SC., et al. Alteration of cytokine profiles in mice exposed to chronic low-dose ionizing radiation. Biochem Biophys Res Commun. 2010 Jul 9;397(4):644-9. doi: 10.1016/j.bbrc.2010.05.121.
Species: Mouse
Sample type:
No posts found
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
G-Series Testing Service
RayBiotech also provides a GLP-compliant CRO testing service for this kit. Fill out the form linked below and get your quote today!
