Mouse Cytokine Array G2

RayBio® G-Series Mouse Cytokine Antibody Array 2 Kit. Detects 32 Mouse Cytokines. Suitable for all liquid sample types.

$540.35
AAM-CYT-G2-4
+

Antigen Information

Gene Symbols:
CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL21 | CCL27 | CCL3 | CCL5 | CSF2   View more

Assay Format

Number of Targets Detected:
32
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
CTACK
(CCL27)
Eotaxin-1
(CCL11)
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IFN-gamma
KC
(CXCL1)
Leptin
MCP-1
(CCL2)
MCP-5
MIP-1 alpha
(CCL3)
MIP-2
MIP-3 beta
(CCL19)
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array G2 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Pelosi L., Berardinelli M., De Pasquale L., et al. Functional and Morphological Improvement of Dystrophic Muscle by Interleukin 6 Receptor Blockade. EBioMedicine. 2015 Feb 26;2(4):285-93. doi: 10.1016/j.ebiom.2015.02.014
    Species: Mouse
    Sample type: Tissue Lysate
  2. Geoffrion, M., Du, X. et al. Differential effects of glyoxalase 1 overexpression on diabetic atherosclerosis and renal dysfunction in streptozotocin?treated, apolipoprotein E?deficient mice. Physiological Reports 2014. Epub Ahead of Print. DOI: 10.14814/phy2.12043
    Species: Mouse
    Sample type: Tissue Lysate
  3. Yang J, Kantrow S, Sai J, et al. INK4a/ARF inactivation with activation of the NF-?B/IL-6 pathway is sufficient to drive the development and growth of angiosarcoma. Cancer research 2012;72(18):4682-4695. doi:10.1158/0008-5472.CAN-12-0440
    Species: Mouse
    Sample type: Serum
  4. Lerner, Immanuel, et al. "Heparanase powers a chronic inflammatory circuit that promotes colitis-associated tumorigenesis in mice." The Journal of clinical investigation 121.5 (2011): 1709-1721.
    Species: Mouse
    Sample type: Conditioned Media
  5. McDonald, Claire L., et al. "Nogo receptor is involved in the adhesion of dendritic cells to myelin." Journal of neuroinflammation 8.1 (2011): 113.
    Species: Mouse
    Sample type: Conditioned Media
  6. Shin SC., et al. Alteration of cytokine profiles in mice exposed to chronic low-dose ionizing radiation. Biochem Biophys Res Commun. 2010 Jul 9;397(4):644-9. doi: 10.1016/j.bbrc.2010.05.121.
    Species: Mouse
    Sample type:

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Analysis Tool Bundle Discount

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1 x $540.35
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70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $656.50
Price for all $575.19

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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