Mouse Cytokine Array G2000

RayBio® G-Series Mouse Cytokine Antibody Array 2000. A combination of Mouse Cytokine Antibody Array G3, G4, & G5 Kit. Detects 144 Mouse Cytokines. Suitable for all liquid sample types.

$2,956.67  
AAM-CYT-G2000-8
+

Assay Format

Number of Targets Detected:
144
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
ACE | AGER | AREG | AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2   View more

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Axl
bFGF
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
Chordin
CRG-2
CTACK
(CCL27)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
CD26
(DPPIV)
Dtk
E-Cadherin
EGF
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
Granzyme B
Gas 1
Gas 6
HAI-1
HGF
HGFR
ICAM-1
(CD54)
IFN-gamma
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IGF-1
IGF-2
IL-1 beta
(IL-1 F2)
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-10
IL-11
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-17B
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-1 alpha
(IL-1 F1)
IL-1 RA
(IL-1 F3)
IL-2
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-9
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MAdCAM-1
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
MMP-2
MMP-3
Neprilysin
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pentraxin-3
(TSG-14)
Platelet Factor 4
(CXCL4)
Prolactin
Pro-MMP-9
P-Selectin
RAGE
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
Sonic Hedgehog N-Terminal
(Shh-N)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TCK-1
(CXCL7)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TREM-1
TROY
(TNFRSF19)
TSLP
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array G2000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Steele C., Karim S., Leach J., et al. CXCR2 Inhibition Profoundly Suppresses Metastases and Augments Immunotherapy in Pancreatic Ductal Adenocarcinoma. Cancer Cell . 2016 Jun 13;29(6):832-845. doi: 10.1016/j.ccell.2016.04.014. Epub 2016 Jun 2.
    Species: Mouse
    Sample type: Conditioned Media (Pancreatic Cancer cells)
  2. Quail D., Olson O., Bhardway P., et al. Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF. Nat Cell Biol . 2017 Aug;19(8):974-987. doi: 10.1038/ncb3578. Epub 2017 Jul 24.
    Species: Mouse
    Sample type: Serum (Obese and normal mouse models)
  3. Ikemoto-Uezumi M., Uezumi A., Tsuchida K., et al. Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration. Stem Cells. 2015 Aug;33(8):2456-68. doi: 10.1002/stem.2045.
    Species: Mouse
    Sample type: Tissue Lysate (tibialis anterior tissue lysate)
  4. Panaroni C., Fulzele K., Saini V., et al. PTH Signaling in Osteoprogenitors Is Essential for B-Lymphocyte Differentiation and Mobilization. J Bone Miner Res. 2015 Dec;30(12):2273-86. doi: 10.1002/jbmr.2581.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (Mone marrow supernates from control and Osx-PPR KO mice)
  5. Liu ML., Nagai T., Tokunaga M., et al. Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction. J Am Heart Assoc. 2014 Dec 2;3(6):e001101. doi: 10.1161/JAHA.114.001101
    Species: Mouse
    Sample type: Conditioned Media (Cardiac progenitor cells, Skeletal myobalstas, and adipose-derived mesenchymal cells)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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