Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
We tested Mouse Cytokine Array G3 kit with mouse tumor tissues. We have obtained very good results. It was clean background, and the mouse tumor tissues was added up 300 ug of total protein into the array slide. Signal of most cytokines were strong.