Mouse Cytokine Array G3

RayBio® G-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.

AAM-CYT-G3-4
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Antigen Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL24 | CCL25   View more

Assay Format

Number of Targets Detected:
62
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Axl
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-9
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
Platelet Factor 4
(CXCL4)
P-Selectin
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VCAM-1
(CD106)
VEGF-A

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array G3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Narasimhulu CA., Selvarajan K., Litvinov D., Parthasarathy S. Anti-Atherosclerotic and Anti-Inflammatory Actions of Sesame Oil. J Med Food 18 (1) 2015, 11-20 DOI: 10.1089/jmf.2014.0138
    Species: Mouse
    Sample type: Plasma
  2. Panaroni C., Fulzele K., Saini V., et al. PTH Signaling in Osteoprogenitors Is Essential for B-Lymphocyte Differentiation and Mobilization. J Bone Miner Res. 2015 Dec;30(12):2273-86. doi: 10.1002/jbmr.2581.
    Species: Mouse
    Sample type: Bone Marrow Aspirate
  3. Koszalka P., Golunska M., Stanislawowski M., et al. CD73 on B16F10 melanoma cells in CD73-deficient mice promotes tumor growth, angiogenesis, neovascularization, macrophage infiltration and metastasis. Int J Biochem Cell Biol. 2015 Dec;69:1-10. doi: 10.1016/j.biocel.2015.10.003.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Alarcon E., Udekwu K., Noel C., et al. Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds. Nanoscale. 2015 Nov 28;7(44):18789-98. doi: 10.1039/c5nr03826j.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Labelle M., et al. Platelets guide the formation of early metastatic niches. PNAS published online July 2014. www.pnas.org/cgi/doi/10.1073/pnas.1411082111
    Species: Mouse
    Sample type: Conditioned Media
  6. Liu ML., Nagai T., Tokunaga M., et al. Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction. J Am Heart Assoc. 2014 Dec 2;3(6):e001101. doi: 10.1161/JAHA.114.001101
    Species: Mouse
    Sample type: Conditioned Media
  7. Benelli R., et al. Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell. Prostate. 2013 Jun;73(9):941-51. doi: 10.1002/pros.22640.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Lei L, Li H, Yan F, Xiao Y (2013) Hyperlipidemia Impaired Innate Immune Response to Periodontal Pathogen Porphyromonas gingivalis in ApolipoproteinE Knockout Mice. PLoS ONE 8(8): e71849. doi:10.1371/journal.pone.0071849
    Species: Mouse
    Sample type: Peritoneal Fluid
  9. Schmitz, K., Pickert, G., Wijnvoord, N., Häussler, A., & Tegeder, I. (2013). Dichotomy of CCL21 and CXCR3 in nerve injury-evoked and autoimmunity-evoked hyperalgesia. Brain, behavior, and immunity, 32, 186-200.
    Species: Mouse
    Sample type: Tissue Lysate
  10. Udagawa, Takeshi, et al. "Vascular Endothelial Growth Factor-D–Mediated Blockade of Regulatory T Cells within Tumors Is Induced by Hematopoietic Stem Cell Transplantation." The Journal of Immunology 191.6 (2013): 3440-3452.
    Species: Mouse
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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