Mouse Cytokine Array G3

RayBio® G-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.

$650.00  
AAM-CYT-G3-4
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Assay Format

Number of Targets Detected:
62
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL24 | CCL25   View more

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Axl
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-9
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
Platelet Factor 4
(CXCL4)
P-Selectin
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VCAM-1
(CD106)
VEGF-A

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array G3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Ionescu L, Byrne RN, van Haaften T, et al. Stem cell conditioned medium improves acute lung injury in mice: in vivo evidence for stem cell paracrine action. American Journal of Physiology - Lung Cellular and Molecular Physiology. 2012;303(11):L967-L977. doi:10.1152/ajplung.00144.2011.
    Species: Mouse
    Sample type: Conditioned Media (Mesenchymal Stem Cell and Fibroblast media)
  2. Litvinov D., et al. Anti-atherosclerotic actions of azelaic acid, an end product of linoleic acid peroxidation, in mice. Atherosclerosis. 2010 Apr;209(2):449-54. doi: 10.1016/j.atherosclerosis.2009.09.076.
    Species: Mouse
    Sample type:
  3. Long C, Hosseinkhani MR, Wang Y, Sriramarao P, Walcheck B. ADAM17 activation in circulating neutrophils following bacterial challenge impairs their recruitment. Journal of Leukocyte Biology 2012;92(3):667-672. doi:10.1189/jlb.0312112.
    Species: Mouse
    Sample type: Peritoneal Fluid (Peritoneal Fluids)
  4. Haddad G, Belosevic M. Transferrin-derived synthetic peptide induces highly conserved pro-inflammatory responses of macrophages. Mol Immunol. 2009;46(4):576-586.
    Species: Mouse
    Sample type: Conditioned Media (Bone Marrow Derived Macrophages cell)
  5. Benelli R., et al. Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell. Prostate. 2013 Jun;73(9):941-51. doi: 10.1002/pros.22640.
    Species: Mouse
    Sample type: Tissue Lysate (TRAMP-G2 tumors, TRAMP-G2LG tumors, and corresponding cell lines)
  6. Lei L, Li H, Yan F, Xiao Y (2013) Hyperlipidemia Impaired Innate Immune Response to Periodontal Pathogen Porphyromonas gingivalis in ApolipoproteinE Knockout Mice. PLoS ONE 8(8): e71849. doi:10.1371/journal.pone.0071849
    Species: Mouse
    Sample type: Peritoneal Fluid (Peritoneal Fluids)
  7. VanSaun, Michael N., Alisha M. Mendonsa, and D. Lee Gorden. "Hepatocellular proliferation correlates with inflammatory cell and cytokine changes in a murine model of nonalchoholic fatty liver disease." PLoS One 8.9 (2013): e73054.
    Species: Mouse
    Sample type: Plasma
  8. VanSaun, Michael N., Alisha M. Mendonsa, and D. Lee Gorden. "Hepatocellular proliferation correlates with inflammatory cell and cytokine changes in a murine model of nonalchoholic fatty liver disease." PLoS One 8.9 (2013): e73054.
    Species: Mouse
    Sample type: Tissue Lysate (liver)
  9. VanSaun, Michael N., Alisha M. Mendonsa, and D. Lee Gorden. "Hepatocellular proliferation correlates with inflammatory cell and cytokine changes in a murine model of nonalchoholic fatty liver disease." PLoS One 8.9 (2013): e73054.
    Species: Mouse
    Sample type: Conditioned Media (CD11b positive myeloid cells )
  10. McBane, Joanne E., et al. "Evaluation of a collagen-chitosan hydrogel for potential use as a pro-angiogenic site for islet transplantation." PloS one 8.10 (2013): e77538.
    Species: Mouse
    Sample type: Tissue Lysate (lysates of hydrogel implants)
We tested Mouse Cytokine Array G3 kit with mouse tumor tissues. We have obtained very good results. It was clean background, and the mouse tumor tissues was added up 300 ug of total protein into the array slide. Signal of most cytokines were strong.
Thanks!
Jun

UTHealth
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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