Mouse Cytokine Array G4

RayBio® G-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.

AAM-CYT-G4-4
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Antigen Information

Gene Symbols:
CCL22 | CXCL11 | CXCL15 | DPP4 | FCGR2B | FGF2 | FIGF | FLT1 | FLT3LG | FLT4   View more

Assay Format

Number of Targets Detected:
34
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
bFGF
CD26
(DPPIV)
Dtk
E-Selectin
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
GITR
(TNFRSF18)
HGFR
ICAM-1
(CD54)
IGFBP-2
IGF-1
IGF-2
IL-15
IL-17 RB
IL-7
I-TAC
(CXCL11)
Lungkine
(CXCL15)
MDC
(CCL22)
MMP-2
MMP-3
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pro-MMP-9
Resistin
Sonic Hedgehog N-Terminal
(Shh-N)
TCK-1
(CXCL7)
TIMP-2
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TSLP
VEGFR1
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array G4 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Narasimhulu CA., Selvarajan K., Litvinov D., Parthasarathy S. Anti-Atherosclerotic and Anti-Inflammatory Actions of Sesame Oil. J Med Food 18 (1) 2015, 11-20 DOI: 10.1089/jmf.2014.0138
    Species: Mouse
    Sample type: Plasma
  2. Panaroni C., Fulzele K., Saini V., et al. PTH Signaling in Osteoprogenitors Is Essential for B-Lymphocyte Differentiation and Mobilization. J Bone Miner Res. 2015 Dec;30(12):2273-86. doi: 10.1002/jbmr.2581.
    Species: Mouse
    Sample type: Bone Marrow Aspirate
  3. Koszalka P., Golunska M., Stanislawowski M., et al. CD73 on B16F10 melanoma cells in CD73-deficient mice promotes tumor growth, angiogenesis, neovascularization, macrophage infiltration and metastasis. Int J Biochem Cell Biol. 2015 Dec;69:1-10. doi: 10.1016/j.biocel.2015.10.003.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Labelle M., et al. Platelets guide the formation of early metastatic niches. PNAS published online July 2014. www.pnas.org/cgi/doi/10.1073/pnas.1411082111
    Species: Mouse
    Sample type: Conditioned Media
  5. Liu ML., Nagai T., Tokunaga M., et al. Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction. J Am Heart Assoc. 2014 Dec 2;3(6):e001101. doi: 10.1161/JAHA.114.001101
    Species: Mouse
    Sample type: Conditioned Media
  6. Benelli R., et al. Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell. Prostate. 2013 Jun;73(9):941-51. doi: 10.1002/pros.22640.
    Species: Mouse
    Sample type: Tissue Lysate
  7. Udagawa, Takeshi, et al. "Vascular Endothelial Growth Factor-D–Mediated Blockade of Regulatory T Cells within Tumors Is Induced by Hematopoietic Stem Cell Transplantation." The Journal of Immunology 191.6 (2013): 3440-3452.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Ionescu L, Byrne RN, van Haaften T, et al. Stem cell conditioned medium improves acute lung injury in mice: in vivo evidence for stem cell paracrine action. American Journal of Physiology - Lung Cellular and Molecular Physiology. 2012;303(11):L967-L977. doi:10.1152/ajplung.00144.2011.
    Species: Mouse
    Sample type: Conditioned Media
  9. Miyauchi S., et al. Oral infection with Porphyromonas gingivalis and systemic cytokine profile in C57BL/6.KOR-ApoEshl mice. J Periodontal Res. 2012 Jun;47(3):402-8. doi: 10.1111/j.1600-0765.2011.01441.x
    Species: Mouse
    Sample type:
  10. Miyauchi S., Maekawa T., Aoki Y., et al. Oral infection with Porphyromonas gingivalis and systemic cytokine profile in C57BL/6.KOR-ApoE shl mice. J Periodontal Res. 2012 Jun;47(3):402-8. doi: 10.1111/j.1600-0765.2011.01441.x.
    Species: Mouse
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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