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Home/Search results/Products/Arrays/Antibody Arrays/G-Series/Cytokine Arrays/Mouse Cytokine Array G4

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Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Accession #: P28843 ELISA Kit: RayBio Mouse DPPIV ELISA	Dtk Gene Symbol: TYRO3 Accession #: O09080 ELISA Kit: RayBio Mouse Dtk ELISA	E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA	Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Accession #: P08101 ELISA Kit: RayBio Mouse Fc-gamma-RIIb ELISA
Flt-3 Ligand Gene Symbol: FLT3LG Accession #: P49772 ELISA Kit: RayBio Mouse Flt-3L ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA	HGFR Gene Symbol: MET Accession #: D3YVY2 ELISA Kit: RayBio Mouse HGF R ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Accession #: P13597 ELISA Kit: RayBio Mouse ICAM-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA
IGF-1 Gene Symbol: IGF1 Accession #: P05018 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGF-2 Gene Symbol: Igf2 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17 RB Gene Symbol: IL17RB Accession #: Q9JIP3 ELISA Kit: RayBio Mouse IL-17B R ELISA	IL-7 Gene Symbol: IL7 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA
I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	Lungkine (CXCL15) Gene Symbol: CXCL15 Accession #: Q3UQ15 ELISA Kit: RayBio Mouse CXCL15 ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MMP-2 Gene Symbol: MMP2 Accession #: P33434 ELISA Kit: RayBio Mouse MMP-2 ELISA	MMP-3 Gene Symbol: MMP3 Accession #: P28862 ELISA Kit: RayBio Mouse MMP-3 ELISA
Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA	Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	Resistin Gene Symbol: RETN Accession #: Q99P87 ELISA Kit: RayBio Mouse Resistin ELISA	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Accession #: ELISA Kit: RayBio Mouse ShhN ELISA
TCK-1 (CXCL7) Gene Symbol: Ppbp Accession #: Q9EQI5 ELISA Kit: RayBio Mouse TCK-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Accession #: Q6GXA8 ELISA Kit: RayBio Mouse TIMP-2 ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Accession #: Q9R1Y0 ELISA Kit: RayBio Mouse TRANCE ELISA	TROY (TNFRSF19) Gene Symbol: TNFRSF19 Accession #: Q80T13 ELISA Kit: RayBio Mouse TROY ELISA	TSLP Gene Symbol: TSLP Accession #: Q9JIE6 ELISA Kit: RayBio Mouse TSLP ELISA
VEGFR1 Gene Symbol: Flt1 Accession #: Q61517 ELISA Kit: RayBio Mouse VEGF R1 ELISA	VEGFR2 Gene Symbol: KDR Accession #: Q3UQZ6 ELISA Kit: RayBio Mouse VEGFR-2 ELISA	VEGFR3 Gene Symbol: FLT4 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA	VEGF-D Gene Symbol: FIGF Accession #: A2AIH3 ELISA Kit: RayBio

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Cytokine Antibody Array G4 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.