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Mouse Cytokine Array GS4000

G-Series Mouse Cytokine Array 4000 Kit. A combination of 5 non-overlapping arrays to measure the relative expression levels of 200 mouse cytokines. Suitable for all liquid sample types.

Old price: $14,376.96
$11,501.57  
You save: $2,875.39 (20%)
GSM-CAA-4000-4
+

Antigen Information

Gene Symbols:
ACE | ADAMTS1 | ADIPOQ | AGER | AHSG | ANGPT4 | ANGPTL1 | ANGPTL3 | ARTN | AXL   View more

Assay Format

Number of Targets Detected:
200
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
Ace
Activin A
ADAMTS-1
(METH1)
Adiponectin
(ACRP30)
ALK-1
Amphiregulin
ANGPTL1
ANGPTL3
Artemin
Axl
BAFF R
Betacellulin
(BTC)
bFGF
BLC
(CXCL13)
C5a
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CCL6
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD32a
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
CD48
(SLAMF2)
CD6
CD80
(B7-1)
Chemerin
Chordin
Clusterin
CRP
(C-Reactive Protein)
CTLA-4
(CD152)
CXCL16
Cystatin C
DAN
Decorin
DKK-1
DLL4
Dtk
E-Cadherin
EDAR
EGF
Endocan
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
Fetuin A
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
Galectin-3
Galectin-7
Gas 1
Gas 6
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
gp130
Granzyme B
Gremlin-1
H60
HAI-1
HGF
HGFR
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
IFN-gamma
IFN-gamma R1
IGF-1
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-1 ra
(IL-1 F3)
IL-10
IL-12 p40
  • Gene Symbol: IL12B
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17 RB
IL-17A
IL-17B
IL-17E
(IL-25)
IL-17F
IL-2
IL-2 R alpha
IL-20
IL-21
IL-22
IL-23 p19
IL-28A
(IFN-lambda 2)
IL-3
IL-3 R beta
IL-33
(IL-1 F11)
IL-4
IL-5
IL-6
IL-7
IL-7 R alpha
IL-9
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Kremen-1
Leptin
Leptin R
Limitin
Lipocalin-2
(NGAL)
LIX
LOX-1
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MAdCAM-1
Marapsin
MBL-2
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
Meteorin
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 gamma
MIP-2
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-10
MMP-2
MMP-3
Neprilysin
Nope
NOV
(CCN3)
Osteoactivin
(GPNMB)
  • Gene Symbol: GPNMB
  • Accession #: Q99P91
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
OX40 Ligand
  • Gene Symbol: TNFSF4
  • Gene ID: 7292
P-Cadherin
PDGF-AA
Pentraxin-3
(TSG-14)
Periostin
Persephin
Platelet Factor 4
(CXCL4)
PlGF-2
Progranulin
Prolactin
Pro-MMP-9
Prostasin
P-Selectin
RAGE
RANTES
(CCL5)
Renin 1
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
sFRP-3
SLAM
(CD150)
Sonic Hedgehog N-Terminal
(Shh-N)
TACI
(TNFRSF13B)
TARC
(CCL17)
TCK-1
(CXCL7)
TECK
(CCL25)
Testican 3
TGF beta 1
  • Gene Symbol: TGFB1
  • Accession #: P04202
Thrombopoietin
(TPO)
TIM-1
(KIM-1)
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TRAIL
(TNFSF10)
Trance
(TNFSF11)
TREM-1
TREML1
(TLT-1)
TROY
(TNFRSF19)
Tryptase epsilon
TSLP
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGF-B
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3
VEGFR1
VEGFR2
VEGFR3

Species Detected

Mouse

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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