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Mouse Cytokine Array Q1

Quantibody® Mouse Cytokine Array 1 Kit. Detects 20 Mouse Cytokines. Suitable for all liquid sample types.

Old price: $1,272.96
$1,018.37  
You save: $254.59 (20%)
QAM-CYT-1-2
+

Antigen Information

Gene Symbols:
CCL2 | CCL5 | CSF1 | CSF2 | CXCL1 | IFNG | IL10 | IL12A | IL13 | IL17A   View more

Assay Format

Pathway:
  • MAPK Signaling
Number of Targets Detected:
20
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
KC
(CXCL1)
MCP-1
(CCL2)
M-CSF
RANTES
(CCL5)
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis." Blood 121.17 (2013): 3345-3363.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (Gata1-low mouse model of myelofibrosis)
  2. Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis." Blood 121.17 (2013): 3345-3363.
    Species: Mouse
    Sample type: Conditioned Media (Gata1-low mouse model of myelofibrosis)
  3. Huggenberger R., et al. Stimulation of lymphangiogenesis via VEGFR-3 inhibits chronic skin inflammation. J Exp Med. 2010 Sep 27;207(10):2255-69. doi: 10.1084/jem.20100559.
    Species: Mouse
    Sample type: Tissue Lysate (Tissue Lysate, Ear skin lysate)
  4. Ojeda, Gloria, et al. "Complement regulatory protein Crry/p65 costimulation expands natural Treg cells with enhanced suppressive properties in proteoglycan?induced arthritis." Arthritis & Rheumatism 63.6 (2011): 1562-1572.
    Species: Mouse
    Sample type: Conditioned Media
  5. Karra L., Haworth O., Priluck R., et al. Lipoxin B4 promotes the resolution of allergic inflammation in the upper and lower airways of mice. Mucosal Immunol. 2014 Dec 3. doi: 10.1038/mi.2014.116.
    Species: Mouse
    Sample type: Serum (Allergic Inflammation)
  6. He S, Zhao J, Xu X, Cui X, Wang N, Han X, Guo Y, Liu Q. Uncovering the Molecular Mechanism of the Qiang-Xin 1 Formula on Sepsis-Induced Cardiac Dysfunction Based on Systems Pharmacology. Oxid Med Cell Longev. 2020 Aug 27;2020:3815185. doi: 10.1155/2020/3815185. PMID: 32908632; PMCID: PMC7474398.
    Species: Mouse
    Sample type: Serum
  7. Arevalo-Martin A., Molina-Holgado E., Guaza C. A CB?/CB? receptor agonist, WIN 55,212-2, exerts its therapeutic effect in a viral autoimmune model of multiple sclerosis by restoring self-tolerance to myelin. Neuropharmacology. 2012 Sep;63(3):385-93. doi: 10.1016/j.neuropharm.2012.04.012.
    Species: Mouse
    Sample type: Tissue Lysate (Mouse brain / CNS and spleen lysates)
  8. Kelley P., et al. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011 Nov;91(11):1643-51. doi: 10.1038/labinvest.2011.121.
    Species: Mouse
    Sample type: Tissue Lysate (Corneal lysates)
  9. Fink DM, Connor AL, Kelley PM, Steele MM, Hollingsworth MA, Tempero RM. Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution. Ng LG, ed. PLoS ONE 2014;9(11):e112737. doi:10.1371/journal.pone.0112737.
    Species: Mouse
    Sample type: Tissue Lysate (Corneal Lysates)
  10. Huang X, Ren L, Ye P, Cheng C, Wu J, et al. (2014) Peroxisome Proliferator Activated Receptor c Deficiency in T Cells Accelerates Chronic Rejection by Influencing the Differentiation of CD4+ T Cells and Alternatively Activated Macrophages. PLoS ONE 9(11): e112953. doi:10.1371/journal.pone.0112953
    Species: Mouse
    Sample type: Conditioned Media (Tissue infiltrating CD4 T cells stimed with anti=CD3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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