Mouse Cytokine Array Q400

Quantibody® Mouse Cytokine Array 400 Kit. A combination of 10 non-overlapping arrays to quantitatively measure 400 Mouse cytokines.

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QAM-CAA-400-1

Antigen Information

Gene Symbols:
Ace | Ace2 | Acvr1b | Adam15 | Adam9 | Adamts1 | Adipoq | Afp | Ahsg | Ambn   View more

Assay Format

Number of Targets Detected:
400
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 6Ckine (CCL21), ACE (CD143), ACE-2, Activin A, Activin R1B (ALK-4), ADAM15, ADAM-9, ADAMTS-1 (METH1), Adiponectin (ACRP30), ADP-Ribosyl Cyclase 2 (BST-1/CD157), ALK-1, Alpha-fetoprotein, Ameloblastin, Amphiregulin, ANGPTL1, ANGPTL3, ANGPTL7, Artemin, ASAHL, ASAM (ACAM), AXL, B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, Betacellulin (BTC), bFGF, BLC (CXCL13), C1q R1 (CD93), C4.4A (LYPD3), C5a, CA12, CA9, Cadherin-4, Carbonic Anhydrase IV (CA4), Carbonic Anhydrase XIV (CA14), Cardiotrophin-1 (CT-1), Cathepsin H, CCL28 (MEC), CCL6, CD117 (c-kit), CD14, CD2, CD200, CD229 (SLAMF3), CD27 (TNFRSF7), CD27 Ligand (TNFSF7), CD28, CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD300b (LMIR5), CD32a, CD36 (SR-B3), CD39 (ENTPD1), CD39L3 (ENTPD3), CD4, CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD44, CD45, CD48 (SLAMF2), CD6, CD69, CD80 (B7-1), CD86 (B7-2), CD90 (Thy1), CD99-L2, CDCP1, CDNF, CEACAM-1, CFVII, Chemerin, CHL-1 (L1CAM-2), Chordin, Chordin-like 2 (CHRDL2), CLEC9A, Clusterin, COCO, Contactin-4 (BIG-2), Contactin-6, CRACC (SLAMF7), Cripto-1, CRP (C-Reactive Protein), CTLA-4 (CD152), CXADR, CXCL14 (BRAK), CXCL16, Cystatin B, Cystatin C, DAN, DcTRAIL R1 (TNFRSF23), Decorin, Dectin-2 (CLEC6A), DKK-1, DLL1, DLL4, DNAM-1 (CD226), DNER, Dtk, E-Cadherin, EDAR, EGF, Endocan, Endoglin (CD105), Endoglycan (PODXL2), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), EphA5, EphA8, EphB2, EphB4, EphB6, Ephrin-A2, Ephrin-A4, Epigen, Epimorphin (Syntaxin 2), Epiregulin, Erythropoietin R, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), FCRL1 (FcRH1), Fetuin A, FGF-10 (KGF-2), FGF-21, FGF-4, FGF-6, FLRG, Flt-3 (Flk-2), Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine (CX3CL1), Frizzled-1, Frizzled-4, Galectin-1, Galectin-3, Galectin-7, Gas 1, Gas 6, GCSF, GCSF R (CD114), GDF-3, GDF-7 (BMP-12), GFR alpha-3, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, gp130 (sgp130), GPV (CD42d), GPVI, Granzyme B, Gremlin-1, GRO alpha (CXCL1), H60, HAI-1, Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3), HGF, HGFR, Histone Deacetylase 8 (HDAC8), I-309 (TCA-3/CCL1), ICAM-1 (CD54), IFN-gamma, IFN-gamma R1, IFN-gamma R2, IGF-1, IGF-2, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGSF8 (CD316), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11 R alpha, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18 BPc, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-20, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-23, IL-23 R, IL-28A (IFN-lambda 2), IL-28A (IFN-lambda 2), IL-3, IL-3 R beta, IL-31, IL-33 (IL-1 F11), IL-34, IL-36 beta (IL-1 F8), IL-4, IL-5, IL-6, IL-7, IL-7 R alpha (CD127), IL-9, I-TAC (CXCL11), JAM-A (CD321/F11R), JAM-C (CD323), Kallikrein 7, Klotho beta, Kremen-1, Kremen-2, Laminin alpha 4, LAMP1 (CD107a), Layilin, LDL R, Leptin, Leptin R, LIF, LIGHT (TNFSF14), Limitin, LIMPII, Lipocalin-2 (NGAL), LIX, LOX-1, LRPAP, LRRC32 (GARP), L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), MAdCAM-1, Marapsin, Mast Cell Protease-11 (Prss34), Mast Cell Protease-6 (Mcpt6), Matrilin-2, Matrilin-3, MBL-2, MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MEP1A, MEPE (OF45), MESDC2, Meteorin, Meteorin-like (METRNL), MFG-E8, MIG (CXCL9), Mimecan (Osteoglycin), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 gamma, MIP-2, MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-10, MMP-2, MMP-3, Nectin-2 (CD112), Nephrin, Neprilysin, Neurocan, Neurturin, NGFR (TNFRSF16), NgR, NKp46 (NCR1), Nope, NOV (CCN3), Olfactomedin-1, Oncostatin M, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteoadherin (Osteomodulin), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 (TNFRSF4), OX40 Ligand (TNFSF4), P-Cadherin, PD-1, PDGF R beta, PDGF-AA, PD-L2 (B7-DC), Pentraxin-3 (TSG-14), Periostin, Persephin, PILR-beta, PLA2G2A, Platelet Factor 4 (CXCL4), Plexin A1, Plexin C1, PLGF-2, Podocalyxin, Podoplanin, Progranulin, Prolactin, Pro-MMP-9, Prostasin, Protocadherin-12, P-Selectin, RAGE, RANK (TNFRSF11A), RANTES (CCL5), Reg2, Relaxin-1, Renin 1, Resistin, Ret, RGM-B, RGM-C (Hemojuvelin), ROBO3, SCF, SDF-1 alpha (CXCL12 alpha), SEMA3F, Semaphorin 3C, Semaphorin 4C, Semaphorin 4F, Semaphorin 4G, Semaphorin 6C, sFRP-3, Siglec-3, Siglec-E, SIGNR1 (CD209b), SLAM (CD150), Slit2, SMOC-1, Sonic Hedgehog N-Terminal (Shh-N), SorCS2, SP-D, SR-AI (MSR), STC-2, Syndecan-1, Syndecan-3, TACI (TNFRSF13B), TARC (CCL17), TCK-1 (CXCL7), TECK (CCL25), Testican 3 (SPOCK3), TFPI-2, TGF beta 1, TGF-beta RI (ALK-5), TGF-beta RII, Thrombopoietin (TPO), Thrombopoietin R (Tpo R), TIGIT, TIM-1 (KIM-1), TIM-3 (KIM-3), TLR2, TNF alpha, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNFRH3 (TNFRSF26), TRAIL (TNFSF10), TRAIL R2 (TNFRSF10B/DR5), TRANCE (TNFSF11), Transglutaminase 2 (TGM2), TREM-1, TREM-2, TREML1 (TLT-1), TRKC, TROP1 (EpCAM), TROP-2, TROY (TNFRSF19), Trypsin 3 (PRSS3), Tryptase epsilon, Tryptase-5, TSLP, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-B, VEGF-D, VEGFR1, VEGFR2, VEGFR3, Wnt-2bMouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q400 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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