Mouse Cytokine Array Q5

Quantibody® Mouse Cytokine Array 5 Kit. Detects 40 Mouse Cytokines. Suitable for all liquid sample types.


Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL12 | CCL17 | CCL2 | CCL24 | CCL3 | CCL5 | CCL9 | CSF1   View more

Assay Format

Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Solid Support:
Glass Slide

Product Specifications

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
CD30 Ligand
Fas Ligand
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40
  • Gene Symbol: IL12B
  • Accession #: P43432
MIP-1 alpha
MIP-1 gamma
Platelet Factor 4
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q5 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Himburg H., Doan P., Quarmyne M., et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. doi: 10.1038/nm.4251.
    Species: Mouse
    Sample type: Bone marrow fluid
  2. Schnepp P., Lee D., Guldner I., et al. GAD1 Upregulation Programs Aggressive Features of Cancer Cell Metabolism in the Brain Metastatic Microenvironment. Cancer Res. 2017 Apr 11. pii: canres.2289.2016. doi: 10.1158/0008-5472.CAN-16-2289.
    Species: Mouse
    Sample type: Conditioned Media
  3. De Pascalis R., Mittereder L., Kennett N., Elkins K. Activities of Murine Peripheral Blood Lymphocytes Provide Immune Correlates That Predict Francisella tularensis Vaccine Efficacy. Infect Immun. 2016 Mar 24;84(4):1054-61. doi: 10.1128/IAI.01348-15.
    Species: Mouse
    Sample type: Conditioned Media
  4. Yariswamy M., Yoshida T., Valente A., et al. Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction. J Biol Chem. 2016 Sep 9;291(37):19425-36. doi: 10.1074/jbc.M116.724138
    Species: Mouse
    Sample type: Tissue Lysate
  5. Kurtz S., Elkins K. Correlates of Vaccine-Induced Protection against Mycobacterium tuberculosis Revealed in Comparative Analyses of Lymphocyte Populations. Clin Vaccine Immunol. 2015 Oct;22(10):1096-108. doi: 10.1128/CVI.00301-15.
    Species: Mouse
    Sample type: Conditioned Media
  6. Jurk D., Wilson C., Passos J., et al. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nature Communications 2, 2014, Article number: 4172. doi:10.1038/ncomms5172
    Species: Mouse
    Sample type: Tissue Lysate
  7. Correnti JM., Cook D., Aksamitiene E., et al. Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modeling. J Physiol. 2014 Nov 10. [Epub ahead of print]. doi: 10.1113/jphysiol.2014.284109.
    Species: Mouse
    Sample type: Serum
  8. Kurtz, Sherry L., et al. "Interleukin-6 is essential for primary resistance to Francisella tularensis live vaccine strain infection." Infection and immunity81.2 (2013): 585-597.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Chappert, Pascal, et al. "Specific gut commensal flora locally alters T cell tuning to endogenous ligands." Immunity 38.6 (2013): 1198-1210.
    Species: Mouse
    Sample type: Serum
  10. Boldrin L, Neal A, Zammit PS, Muntoni F, Morgan JE. Donor Satellite Cell Engraftment is Significantly Augmented When the Host Niche is Preserved and Endogenous Satellite Cells are Incapacitated. Stem Cells (Dayton, Ohio) 2012;30(9):1971-1984. doi:10.1002/stem.1158.
    Species: Mouse
    Sample type: Tissue Lysate

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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