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Home/Search results/Products/Arrays/Antibody Arrays/Quantibody/Cytokine Arrays/Mouse Cytokine Array Q5

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Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:

CCL1 | CCL11 | CCL12 | CCL17 | CCL2 | CCL24 | CCL3 | CCL5 | CCL9 | CSF1 View more

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA
Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Accession #: P13597 ELISA Kit: RayBio Mouse ICAM-1 ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA
IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA
IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-7 Gene Symbol: IL7 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Accession #: P43432
IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971 ELISA Kit: RayBio Mouse IL-17A ELISA	IL-21 Gene Symbol: IL21 Accession #: E9PX58 ELISA Kit: RayBio Mouse IL-21 ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA
Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA
MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA
TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Mouse Cytokine Array Q5 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.