Mouse Cytokine Array Q640

Quantibody® Mouse Cytokine Array 640 Kit. A combination of 16 non-overlapping arrays to quantitatively measure 640 Mouse cytokines.

[Price not listed]  
QAM-CAA-640-1

Antigen Information

Gene Symbols:
Ace | Ace2 | Acvr1b | Adam15 | Adam9 | Adamts1 | Adipoq | Afp | Agpt2 | AGT   View more

Assay Format

Number of Targets Detected:
640
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

15-PGDH (HPGD), 2B4 (CD244), 4-1BB Ligand (TNFSF9), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), A33, ACE (CD143), ACE-2, Activin A, Activin R1B (ALK-4), ADAM15, ADAM-9, ADAMTS-1 (METH1), Adiponectin (ACRP30), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), ALK-1, Alpha-fetoprotein, Ameloblastin, AMICA, Amphiregulin, Angiogenin, Angiopoietin-2, ANGPTL1, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL7, APCS, ARSA, ART4, Artemin, Arylsulfatase G, ASAHL, ASAM (ACAM), ASGP-R 1 (ASGR1), AXL, B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, BAMBI (NMA), BCHE, Bcl-xL, BDNF, Betacellulin (BTC), beta-NGF, bFGF, BID, BLC (CXCL13), BMP-5, BMP-6, BMP-7, BMP-9, BMPR-II, BTLA (CD272), BTN1A1 (Butyrophilin), C1q R1 (CD93), C4.4A (LYPD3), C5a, CA12, CA2, CA9, Cadherin-4, CAMK4, Carbohydrate Sulfotransferase 7 (CHST7), Carbonic Anhydrase IV (CA4), Carbonic Anhydrase XIV (CA14), Carboxylesterase 1 (CES1), Carboxylesterase 2 (CES2), Carboxypeptidase A1 (CPA1), Carboxypeptidase B1 (CPB1), Carboxypeptidase M (CPM), Cardiotrophin-1 (CT-1), Carnosine Dipeptidase-1 (CNDP1), Cathepsin H, Cathepsin L, CCL28 (MEC), CCL6, CD117 (c-kit), CD14, CD147 (EMMPRIN), CD155 (PVR), CD160, CD164, CD180, CD2, CD200, CD200 R1, CD200R4, CD229 (SLAMF3), CD27 Ligand (TNFSF7), CD27 (TNFRSF7), CD28, CD2F-10 (SLAMF9), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD300b (LMIR5), CD32a, CD34, CD36 (SR-B3), CD38, CD39 (ENTPD1), CD39L3 (ENTPD3), CD4, CD40 Ligand (TNFSF5), CD40 (TNFRSF5), CD44, CD45, CD47, CD48 (SLAMF2), CD5, CD53, CD59a (MAC-IP), CD6, CD69, CD7, CD74, CD80 (B7-1), CD86 (B7-2), CD90 (Thy1), CD99, CD99-L2, CDCP1, CDNF, CEACAM-1, Cerberus 1, CFVII, Chemerin, CHL-1 (L1CAM-2), Chordin, Chordin-like 2 (CHRDL2), CHST3, CHST4 (GST-3), CLEC4F (CLECSF13), CLEC9A, Clusterin, CMG-2 (ANTXR2), CNTF, CNTN2, Coagulation Factor III (Tissue Factor/Thromboplastin), COCO, Collectin-12 (CL-P1), Complement Component C1ra, Contactin-1, Contactin-4 (BIG-2), Contactin-6, CRACC (SLAMF7), CREG, CRELD1, CRELD2, Cripto-1, CRISP-4, CRP (C-Reactive Protein), CST7, CTLA-4 (CD152), CXADR, CXCL14 (BRAK), CXCL16, CXCL17 (VCC-1), Cystatin B, Cystatin C, DAN, DCC, DcTRAIL R1 (TNFRSF23), DcTRAIL R2 (TNFRSF22), Decorin, Dectin-1 (CLEC7A), Dectin-2 (CLEC6A), DKK-1, Dkk-2, DLL1, DLL4, DNAM-1 (CD226), DNER, DR3 (TNFRSF25), DSPG3, Dtk, E-Cadherin, EDAR, EGF, EG-VEGF (PK1), Endocan, Endoglin (CD105), Endoglycan (PODXL2), ENPP-2 (Autotaxin), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), EPCR, EphA1, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB2, EphB3, EphB4, EphB6, Ephrin-A2, Ephrin-A4, Ephrin-B1, Ephrin-B2, Epigen, Epimorphin (Syntaxin 2), Epiregulin, ErbB2, Erythropoietin, Erythropoietin R, E-Selectin, FABP4 (A-FABP), FAM3C, Fas Ligand (TNFSF6), Fas (TNFRSF6/Apo-1), FCER2, FCRL1 (FcRH1), FCRL5 (FcRH5), FCRN, FDPS, Fetuin A, FGF R3, FGF R4, FGF R5 (FGFRL1), FGF-10 (KGF-2), FGF-21, FGF-23, FGF-4, FGF-6, FGF-8, FLRG, Flt-3 Ligand, Flt-3 (Flk-2), Follistatin, Follistatin-like 1, FOLR1, Fractalkine (CX3CL1), Frizzled-1, Frizzled-2, Frizzled-4, Frizzled-9, Galectin-1, Galectin-2, Galectin-3, Galectin-4, Galectin-7, Galectin-9, GAPDH, Gas 1, Gas 6, GCSF, GCSF R (CD114), GDF-11 (BMP-11), GDF-3, GDF-5 (BMP-14), GDF-7 (BMP-12), GDF-8 (Myostatin), GDF-9, GFR alpha-2, GFR alpha-3, GITR Ligand (TNFSF18), GITR (TNFRSF18), Glypican 5, GM-CSF, gp130 (sgp130), GPV (CD42d), GPVI, Granzyme B, Gremlin-1, GRO alpha (CXCL1), Growth Hormone R, H60, HABP1 (C1qBP), HAI-1, HAI-2, Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3), HGF, HGFR, HIN-1 (SCGB3A1), Histone Deacetylase 8 (HDAC8), HN1, Human Amyloid-like protein 1 (APLP-1), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-5, ICOS, Iduronate 2-Sulfatase (IDS), IFNA2, IFNAR1, IFN-beta, IFN-gamma, IFN-gamma R1, IFN-gamma R2, IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGFBP-L1, IGSF4A (SynCAM1), IGSF4B (SynCAM3/CADM3), IGSF8 (CD316), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 F9 (IL-36 gamma), IL-1 R1, IL-1 R2, IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11 R alpha, IL-12 p40/p70, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18 BPc, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R gamma (Common gamma Chain), IL-20, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 R alpha 1, IL-23, IL-23 R, IL-27 p28 (IL-30), IL-27 R alpha, IL-28A (IFN-lambda 2), IL-28A (IFN-lambda 2), IL-3, IL-3 R alpha, IL-3 R beta, IL-31, IL-33 (IL-1 F11), IL-34, IL-36 alpha (IL-1 F6), IL-36 beta (IL-1 F8), IL-4, IL-4 R alpha, IL-5, IL-5 R alpha, IL-6, IL-7, IL-7 R alpha (CD127), IL-9, IP-10 (CXCL10), I-TAC (CXCL11), JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), JAM-C (CD323), JNK1, Kallikrein 7, Kirrel2 (NEPH3), Klotho beta, KLRB1A, KLRC1, Kremen-1, Kremen-2, Kynureninase, LAG-3, LAIR1, Laminin alpha 4, LAMP1 (CD107a), Latexin, Layilin, LDL R, Lefty-1, Legumain, Leptin, Leptin R, LIF, LIGHT (TNFSF14), LILRC1, Limitin, LIMPII, Lipocalin-2 (NGAL), LIX, LOX-1, LRIG1, LRPAP, LRRC32 (GARP), L-Selectin (CD62L), LTA4H, Lungkine (CXCL15), Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), MAdCAM-1, MANF, Marapsin, Mast Cell Protease-1 (Mcpt1), Mast Cell Protease-11 (Prss34), Mast Cell Protease-6 (Mcpt6), Matrilin-2, Matrilin-3, Matrilin-4, MBL-2, MCAM (CD146), MCP-1 (CCL2), MCP-5, Mcpt7, M-CSF, MD-1 (LY86), MDC (CCL22), MDL-1 (CLEC5A), MEP1A, MEPE (OF45), Meprin beta (MEP1B), Mer, MESDC2, Meteorin, Meteorin-like (METRNL), MFG-E8, MFI2, MGL1, MGL2 (CD301b), MIG (CXCL9), Mimecan (Osteoglycin), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 gamma, MIP-2, MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-10, MMP-2, MMP-3, MMP-7, MMP-8, MOG, MSP Receptor (Ron), NAALADL1, NCAM-1 (CD56), Nectin-2 (CD112), Nectin-4, Nephrin, Neprilysin, Netrin-1, Netrin-4, Netrin-G1a, Netrin-G2a, Neurocan, Neuroglycan C (CSPG5), Neuroplastin, Neurturin, NGFR (TNFRSF16), NgR, Nicastrin (NCSTN), Nidogen-2, NKp46 (NCR1), Noggin, Nope, NOV (CCN3), NTB-A (SLAMF6), Olfactomedin-1, Oncostatin M, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteoadherin (Osteomodulin), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), OX40 (TNFRSF4), PAI-1, P-Cadherin, PD-1, PDGF R alpha, PDGF R beta, PDGF-AA, PD-L2 (B7-DC), Pentraxin-3 (TSG-14), Periostin, Persephin, PILR-alpha, PILR-beta, PIR-B, PLA2G2A, Platelet Factor 4 (CXCL4), Plexin A1, Plexin B2, Plexin C1, PLGF-2, Podocalyxin, Podoplanin, PPM1A, PREP, Progranulin, Prolactin, Pro-MMP-9, Proprotein Convertase 9 (PCSK9), Prostasin, Protocadherin-12, P-Selectin, PSMB6, PTK6, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Reg2, Relaxin-1, Renin 1, Resistin, Ret, RGM-A, RGM-B, RGM-C (Hemojuvelin), ROBO3, R-Spondin 1, R-Spondin 4, Ryk, S100A1, S100A3, S100A6, S100A9, SCF, SDC4, SDF-1 alpha (CXCL12 alpha), SELPLG, SEMA3F, Semaphorin 3C, Semaphorin 4C, Semaphorin 4F, Semaphorin 4G, Semaphorin 6A, Semaphorin 6C, Serpin A6, Serpin B8, Serpina10, Serpina3c, Serpinb10, SERPINF1 (PEDF), sFRP-2, sFRP-3, Siglec-1 (CD169), Siglec-2, Siglec-3, Siglec-E, Siglec-F, SIGNR1 (CD209b), SIRP alpha, SIRP beta 1, SLAM (CD150), Slit2, SMOC-1, Sonic Hedgehog N-Terminal (Shh-N), SorCS2, SP-D, SR-AI (MSR), ST6GALNAC2, STC-2, Syndecan-1, Syndecan-3, TACI (TNFRSF13B), TARC (CCL17), TCAM-1, TCK-1 (CXCL7), TECK (CCL25), Testican 3 (SPOCK3), TFPI-2, TGF beta 1, TGF-beta RI (ALK-5), TGF-beta RII, Thimet Oligopeptidase (THOP1), Thrombomodulin, Thrombopoietin R (Tpo R), Thrombopoietin (TPO), Thrombospondin 2, Tie-2, TIGIT, TIM-1 (KIM-1), TIM-3 (KIM-3), TIM-4, TIMP-4, TLR1, TLR2, TLR6, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNFRH3 (TNFRSF26), TRAIL R2 (TNFRSF10B/DR5), TRAIL (TNFSF10), TRANCE (TNFSF11), Transferrin, Transglutaminase 2 (TGM2), TREM-1, TREM-2, TREML1 (TLT-1), TRKC, TROP1 (EpCAM), TROP-2, TROY (TNFRSF19), Trypsin 3 (PRSS3), Tryptase epsilon, Tryptase-5, TSC22D1, TSLP, TSLP R, TSP-4, TWEAK R (TNFRSF12), TWEAK (TNFSF12), UCH-L1, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-B, VEGF-D, VEGFR1, VEGFR2, VEGFR3, VISTA (B7-H5/PD-1H), VLDL-R, VNN1, VSIG4, WISP-1 (CCN4), Wnt-2b, Wnt-8a

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q640 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

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Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.