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Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
6Ckine (CCL21) Gene Symbol: CCL21 Accession #: P86792 ELISA Kit: RayBio Mouse 6Ckine ELISA	Activin A Gene Symbol: INHBA Accession #: Q04998 ELISA Kit: RayBio Mouse Activin A ELISA	ADAMTS-1 (METH1) Gene Symbol: ADAMTS1 Accession #: P97857 ELISA Kit: RayBio Mouse ADAMTS1 ELISA	Adiponectin (ACRP30) Gene Symbol: ADIPOQ Accession #: Q62400 ELISA Kit: RayBio Mouse Adiponectin ELISA	ANGPTL1 Gene Symbol: ANGPTL1 Accession #: Q640P2 ELISA Kit: RayBio Mouse ANGPTL1 ELISA
ANGPTL3 Gene Symbol: ANGPTL3 Accession #: Q9R182 ELISA Kit: RayBio Mouse ANGPTL3 ELISA	Artemin Gene Symbol: ARTN Accession #: Q3SXF4 ELISA Kit: RayBio Mouse Artemin ELISA	CCL28 (MEC) Gene Symbol: CCL28 Accession #: Q9JIL2 ELISA Kit: RayBio Mouse CCL28 ELISA	CD36 (SR-B3) Gene Symbol: CD36 Accession #: Q08857 ELISA Kit: RayBio Mouse CD36 ELISA	Chordin Gene Symbol: CHRD Accession #: Q9Z0E2 ELISA Kit: RayBio Mouse Chordin ELISA
CRP (C-Reactive Protein) Gene Symbol: CRP Accession #: P14847 ELISA Kit: RayBio Mouse CRP ELISA	E-Cadherin Gene Symbol: CDH1 Accession #: P09803 ELISA Kit: RayBio Mouse E-Cadherin ELISA	Epigen Gene Symbol: EPGN Accession #: Q8CEX5 ELISA Kit: RayBio Mouse Epigen ELISA	Epiregulin Gene Symbol: EREG Accession #: Q61521 ELISA Kit: RayBio Mouse Epiregulin ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Accession #: Q6GT31 ELISA Kit: RayBio Mouse FAS ELISA
Galectin-7 Gene Symbol: LGALS7 Accession #: Q9CRB1 ELISA Kit: RayBio Mouse Galectin-7 ELISA	gp130 Gene Symbol: Il6st Accession #: Q00560 ELISA Kit: RayBio Mouse gp130 ELISA	Granzyme B Gene Symbol: GZMB Accession #: Q3V2P1 ELISA Kit: RayBio Mouse Granzyme B ELISA	Gremlin-1 Gene Symbol: GREM1 Accession #: O70326 ELISA Kit: RayBio Mouse Gremlin-1 ELISA	IFN-gamma R1 Gene Symbol: IFNGR1 Accession #: P15261 ELISA Kit: RayBio Mouse IFN-gamma R1 ELISA
IL-17 RB Gene Symbol: IL17RB Accession #: Q9JIP3 ELISA Kit: RayBio Mouse IL-17B R ELISA	IL-17B Gene Symbol: IL17B Accession #: Q99MY3 ELISA Kit: RayBio Mouse IL-17B ELISA	IL-22 Gene Symbol: IL22 Accession #: Q14BB3 ELISA Kit: RayBio Mouse IL-22 ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 Accession #: P14097 ELISA Kit: RayBio Mouse MIP-1beta ELISA	MMP-10 Gene Symbol: MMP10 Accession #: O55123 ELISA Kit: RayBio Mouse MMP-10 ELISA
MMP-2 Gene Symbol: MMP2 Accession #: P33434 ELISA Kit: RayBio Mouse MMP-2 ELISA	MMP-3 Gene Symbol: MMP3 Accession #: P28862 ELISA Kit: RayBio Mouse MMP-3 ELISA	PDGF-AA Gene Symbol: PDGFA Accession #: P20033 ELISA Kit: RayBio Mouse PDGF-AA ELISA	Persephin Gene Symbol: PSPN Accession #: A1L3Q1 ELISA Kit: RayBio Mouse Persephin ELISA	sFRP-3 Gene Symbol: FRZB Accession #: O09075 ELISA Kit: RayBio Mouse sFRP-3 ELISA
SLAM (CD150) Gene Symbol: SLAMF1 Accession #: Q9QXZ3 ELISA Kit: RayBio Mouse SLAM ELISA	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH ELISA Kit: RayBio Mouse ShhN ELISA	TCK-1 (CXCL7) Gene Symbol: Ppbp Accession #: Q9EQI5 ELISA Kit: RayBio Mouse TCK-1 ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA	TGF beta 1 Gene Symbol: TGFB1 Accession #: P04202
Trance (TNFSF11) Gene Symbol: TNFSF11 Accession #: Q9R1Y0 ELISA Kit: RayBio Mouse TRANCE ELISA	TREML1 (TLT-1) Gene Symbol: TREML1 Accession #: Q8K558 ELISA Kit: RayBio Mouse TREML1 ELISA	TWEAK (TNFSF12) Gene Symbol: TNFSF12 Accession #: O54907 ELISA Kit: RayBio Mouse TWEAK ELISA	VEGF-B Gene Symbol: VEGFB ELISA Kit: RayBio Mouse VEGF-B ELISA	VEGFR2 Gene Symbol: KDR Accession #: Q3UQZ6 ELISA Kit: RayBio Mouse VEGFR-2 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection
Biomarker Screening
Identifying Key Factors
Confirming Biological Process
Biomarker Validation
Validation of Antibody Array Results
Quantitative Protein Detection
Establishing Normal Range

Kit Components

Mouse Cytokine Array Q8 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.