RayBio® Mouse GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P09920
Gene ID:
  • 12985
Gene Symbols:
  • CSF3
  • CSFG
Protein Name & Synonyms:
Granulocyte colony-stimulating factor (G-CSF)
Target Species:
  • Mouse

Assay Format

Detection Range:
0.5 pg/ml - 150 pg/ml
Recommended Dilution (serum & plasma):
20 - 100 fold
0.5 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Li Z., Wang L., Yi L., et al. Succinate ester derivative of ?-tocopherol enhances the protective effects against 60Co ?-ray-induced hematopoietic injury through granulocyte colony-stimulating factor induction in mice. Sci Rep. 2017; 7: 40380. doi: 10.1038/srep40380
    Species: Mouse
    Sample type: Serum
  2. Hollmen M., Karaman S., Schwager S., et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology. 2016 Mar; 5(3): e1115177.
    Species: Mouse
    Sample type: Conditioned Media
  3. Zhang H., Ren Y., Tang X., et al. Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis. Sci Rep. 2015 Mar 9;5:8888. doi: 10.1038/srep08888.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Pathak R., Shao L., Ghosh S., et al. Thrombomodulin contributes to gamma tocotrienol-mediated lethality protection and hematopoietic cell recovery in irradiated mice. PLoS One. 2015 Apr 10;10(4):e0122511. doi: 10.1371/journal.pone.0122511
    Species: Mouse
    Sample type: Plasma
  5. Andzinski L., Wu C., Lienenklaus S., Kroger A., Weiss S., Jablonska J. Delayed apoptosis of tumor associated neutrophils in the absence of endogenous IFN-?. International Journal of Cancer. Pubslihed Online, DOI: 10.1002/ijc.28957 [Epub ahead of Print]
    Species: Mouse
    Sample type: Serum
  6. Mishra S., et al. Semiquinone Glucoside Derivative Provides Protection Against c-Radiation by Modulation of Immune Response in Murine Model. Environmental Toxicology, Epub ahead of print Oct 2014.DOI: 10.1002/tox.22061
    Species: Mouse
    Sample type: Serum
  7. Xu X., Shao B., Wang R., Zhou S., Tang Z., Lu W., Xiong S. Role of Interleukin-17 in defense against pseudomonas aeruginosa infection in lungs. Int J Clin Exp Med 2014;7(4):809-816
    Species: Mouse
    Sample type: BAL Fluid
  8. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling. Blood. 2013;121(6):930-939.
    Species: Mouse
    Sample type: Serum
  9. Fortin, Audrey, et al. "A soluble granulocyte colony stimulating factor decoy receptor as a novel tool to increase hematopoietic cell homing and reconstitution in mice."?Stem cells and development?22.6 (2012): 975-984.
    Species: Mouse
    Sample type: Bone Marrow
  10. Lee D., Wolstein J., Pudasaini B., Plotkin M. INK4a deletion results in improved kidney regeneration and decreased capillary rarefaction after ischemia-reperfusion injury. Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F183-91. doi: 10.1152/ajprenal.00407.2011.
    Species: Mouse
    Sample type: Plasma

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