Mouse Growth Factor Array C3

RayBio® C-Series Mouse Growth Factor Antibody Array 3 Kit. Detects 30 Mouse Growth Factors. Suitable for all liquid sample types.

AAM-GF-3-2
+

Antigen Information

Gene Symbols:
AREG | CSF1 | CSF2 | CSF3 | EGF | EGFR | FGF2 | FGF7 | FIGF | FLT1   View more

Assay Format

Pathway:
  • IGF Signaling
Number of Targets Detected:
30
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Amphiregulin
beta-NGF
bFGF
EGF
EGFR
FGF-7
(KGF)
GCSF
GM-CSF
HGF
HGFR
IGF-1
IGF-1 R
IGF-2
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IL-2
IL-7
M-CSF
PDGF-AA
PDGF-BB
PLGF-2
SCF
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 21803
  • Accession #: P04202
VEGF-A
VEGF-D
  • Gene Symbol: FIGF VEGFD
  • Gene ID: 14205
  • Accession #: P97946
VEGFR1
VEGFR2
VEGFR3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Growth Factor Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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