RayBio® Mouse HGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

400 pg/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
400 pg/ml - 60 ng/ml
Recommended Dilution (serum & plasma)
2 - 10 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data


This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Protein Name & Synonyms
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF)
Accession Number
Gene Names
Gene ID

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • HGF

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Method of Detection:
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample Type: Tissue Lysate

  2. Shibasaki S., Kitano S., Karasaki M., et al. Blocking c-Met signaling enhances bone morphogenetic protein-2-induced osteoblast differentiation. FEBS Open Bio. 2015 Apr 20;5:341-7. doi: 10.1016/j.fob.2015.04.008.
    Species: Mouse
    Sample Type: Conditioned Media

  3. Rehm, A. et al. Dendritic cell-mediated survival signals inEm-Myc B-cell lymphoma depend on the transcription factor C/EBPb. Nat. Commun.5:5057 doi: 10.1038/ncomms6057 (2014).
    Species: Human
    Sample Type: Conditioned Media

  4. Díaz-Coránguez M, Segovia J, López-Ornelas A, et al. Transmigration of Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells. PLoS ONE. 2013;8(4):e60655. doi:10.1371/journal.pone.0060655
    Species: Rat
    Sample Type: Conditioned Media

  5. Yamamoto Y, Fujita M, Tanaka Y, et al. Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue-Derived Stromal Cells. BioRes Open Access. 2013 May 2;:130502062239007.
    Species: Mouse
    Sample Type: Conditioned Media

  6. Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.
    Species: Mouse
    Sample Type: Conditioned Media

  7. Li L., Black R., Ma Z., et al. Use of mouse hematopoietic stem and progenitor cells to treat acute kidney injury. Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F9-F19. doi: 10.1152/ajprenal.00377.2011.
    Species: Mouse
    Sample Type: Conditioned Media

  8. Talbot N., Sparks W., Powell A., et al. Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):1-11. doi: 10.1007/s11626-011-9467-7
    Species: Mouse
    Sample Type: Conditioned Media

  9. Sato C., Iwasaki T., Kitano S., et al. Sphingosine 1-phosphate receptor activation enhances BMP-2-induced osteoblast differentiation. Biochem Biophys Res Commun. 2012 Jun 22;423(1):200-5. doi: 10.1016/j.bbrc.2012.05.130.
    Species: Mouse
    Sample Type: Conditioned Media

  10. Hora, Caroline, Pamela Romanque, and Jean‐François F. Dufour. "Effect of sorafenib on murine liver regeneration." Hepatology 53.2 (2011): 577-586.

Write a review

No posts found

U.S. International


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Cart content Cart content