RayBio® Mouse HGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • Q08048
Gene ID:
  • 15234
Gene Symbols:
  • HGF
Protein Name & Synonyms:
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain]
Target Species:
  • Mouse

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Shibasaki S., Kitano S., Karasaki M., et al. Blocking c-Met signaling enhances bone morphogenetic protein-2-induced osteoblast differentiation. FEBS Open Bio. 2015 Apr 20;5:341-7. doi: 10.1016/j.fob.2015.04.008.
    Species: Mouse
    Sample type: Conditioned Media
  3. Rehm, A. et al. Dendritic cell-mediated survival signals inEm-Myc B-cell lymphoma depend on the transcription factor C/EBPb. Nat. Commun.5:5057 doi: 10.1038/ncomms6057 (2014).
    Species: Human
    Sample type: Conditioned Media
  4. Díaz-Coránguez M, Segovia J, López-Ornelas A, et al. Transmigration of Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells. PLoS ONE. 2013;8(4):e60655. doi:10.1371/journal.pone.0060655
    Species: Rat
    Sample type: Conditioned Media
  5. Yamamoto Y, Fujita M, Tanaka Y, et al. Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue-Derived Stromal Cells. BioRes Open Access. 2013 May 2;:130502062239007.
    Species: Mouse
    Sample type: Conditioned Media
  6. Xinaris, Christodoulos, et al. "A novel strategy to enhance mesenchymal stem cell migration capacity and promote tissue repair in an injury specific fashion."?Cell Transplantation?22.3 (2013): 423-436.
    Species: Mouse
    Sample type: Conditioned Media
  7. Yamamoto, Yoritsuna, et al. "Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue?Derived Stromal Cells."?BioResearch open access?2.3 (2013): 199-205.
    Species: Mouse
    Sample type: Conditioned Media
  8. Hamdi, Hadhami, et al. "Efficacy of epicardially delivered adipose stroma cell sheets in dilated cardiomyopathy."?Cardiovascular research?99.4 (2013): 640-647.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.
    Species: Mouse
    Sample type: Conditioned Media
  10. Li L., Black R., Ma Z., et al. Use of mouse hematopoietic stem and progenitor cells to treat acute kidney injury. Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F9-F19. doi: 10.1152/ajprenal.00377.2011.
    Species: Mouse
    Sample type: Conditioned Media

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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