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| CODE | Sample Type | Number of Plates | Price | ||
|---|---|---|---|---|---|
|
ELM-HGF-5
|
$1,608.00 | |||
|
|
ELM-HGF-CL-1
|
$402.00 | |||
|
|
ELM-HGF-2
|
$723.60 | |||
|
|
ELM-HGF-1
|
$402.00 | |||
|
|
ELM-HGF-CL-5
|
$1,608.00 | |||
|
|
ELM-HGF-CL-2
|
$723.60 |
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Antigen Information
Accession Number:
- Q08048
Gene ID:
- 15234
Gene Symbols:
- HGF
Protein Name & Synonyms:
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain]
Target Species:
- Mouse
Assay Format
Detection Range:
400 pg/ml - 60 ng/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
400 pg/ml
Need more sensitivity? Check out the new IQELISA&tra
Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
Species: Mouse
Sample type: Tissue Lysate -
Shibasaki S., Kitano S., Karasaki M., et al. Blocking c-Met signaling enhances bone morphogenetic protein-2-induced osteoblast differentiation. FEBS Open Bio. 2015 Apr 20;5:341-7. doi: 10.1016/j.fob.2015.04.008.
Species: Mouse
Sample type: Conditioned Media -
Rehm, A. et al. Dendritic cell-mediated survival signals inEm-Myc B-cell lymphoma depend on the transcription factor C/EBPb. Nat. Commun.5:5057 doi: 10.1038/ncomms6057 (2014).
Species: Human
Sample type: Conditioned Media -
Díaz-Coránguez M, Segovia J, López-Ornelas A, et al. Transmigration of Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells. PLoS ONE. 2013;8(4):e60655. doi:10.1371/journal.pone.0060655
Species: Rat
Sample type: Conditioned Media -
Yamamoto Y, Fujita M, Tanaka Y, et al. Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue-Derived Stromal Cells. BioRes Open Access. 2013 May 2;:130502062239007.
Species: Mouse
Sample type: Conditioned Media -
Xinaris, Christodoulos, et al. "A novel strategy to enhance mesenchymal stem cell migration capacity and promote tissue repair in an injury specific fashion."?Cell Transplantation?22.3 (2013): 423-436.
Species: Mouse
Sample type: Conditioned Media -
Yamamoto, Yoritsuna, et al. "Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue?Derived Stromal Cells."?BioResearch open access?2.3 (2013): 199-205.
Species: Mouse
Sample type: Conditioned Media -
Hamdi, Hadhami, et al. "Efficacy of epicardially delivered adipose stroma cell sheets in dilated cardiomyopathy."?Cardiovascular research?99.4 (2013): 640-647.
Species: Mouse
Sample type: Tissue Lysate -
Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.
Species: Mouse
Sample type: Conditioned Media -
Li L., Black R., Ma Z., et al. Use of mouse hematopoietic stem and progenitor cells to treat acute kidney injury. Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F9-F19. doi: 10.1152/ajprenal.00377.2011.
Species: Mouse
Sample type: Conditioned Media
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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