Mouse HGF ELISA

RayBio® Mouse HGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELM-HGF-CL-1
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Assay Format

Detection Range:
400 pg/ml - 60 ng/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
400 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q08048
Gene ID:
  • 15234
Gene Symbols:
  • HGF
Protein Name & Synonyms:
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain]
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Hora, Caroline, Pamela Romanque, and Jean?François F. Dufour. "Effect of sorafenib on murine liver regeneration." Hepatology 53.2 (2011): 577-586.
    Species: Mouse
    Sample type: Tissue lysate
  2. Rehm, A. et al. Dendritic cell-mediated survival signals inEm-Myc B-cell lymphoma depend on the transcription factor C/EBPb. Nat. Commun.5:5057 doi: 10.1038/ncomms6057 (2014).
    Species: Human
    Sample type: Conditioned Media
  3. Hamdi, Hadhami, et al. "Efficacy of epicardially delivered adipose stroma cell sheets in dilated cardiomyopathy." Cardiovascular research 99.4 (2013): 640-647.
    Species: Mouse
    Sample type: Tissue Lysate (dilated cardiomyopathy model)
  4. Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.
    Species: Mouse
    Sample type: Conditioned Media (MLO-Y4 osteocytes cells)
  5. Li L., Black R., Ma Z., et al. Use of mouse hematopoietic stem and progenitor cells to treat acute kidney injury. Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F9-F19. doi: 10.1152/ajprenal.00377.2011.
    Species: Mouse
    Sample type: Conditioned Media
  6. Xinaris, Christodoulos, et al. "A novel strategy to enhance mesenchymal stem cell migration capacity and promote tissue repair in an injury specific fashion." Cell Transplantation 22.3 (2013): 423-436.
    Species: Mouse
    Sample type: Conditioned Media (MSCs (AKI injury repair model))
  7. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample type: Tissue Lysate (Skeletal muscle lysate from plantaris muscle)
  8. Sato C., Iwasaki T., Kitano S., et al. Sphingosine 1-phosphate receptor activation enhances BMP-2-induced osteoblast differentiation. Biochem Biophys Res Commun. 2012 Jun 22;423(1):200-5. doi: 10.1016/j.bbrc.2012.05.130.
    Species: Mouse
    Sample type: Conditioned Media (C2C12 cells, mouse myoblast cell line and role of S1P)
  9. Díaz-Coránguez M, Segovia J, López-Ornelas A, et al. Transmigration of Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells. PLoS ONE. 2013;8(4):e60655. doi:10.1371/journal.pone.0060655
    Species: Rat
    Sample type: Conditioned Media (astrocytes, Glioma C6)
  10. Talbot N., Sparks W., Powell A., et al. Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):1-11. doi: 10.1007/s11626-011-9467-7
    Species: Mouse
    Sample type: Conditioned Media (Primary CF-1 fibtoblasts from 13.5d fetsus of CF-1 mice, and STO fibroblasts)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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