Mouse IFN-gamma ELISA

RayBio® Mouse IFN-gamma ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Old price: $420.00
$336.00  
You save: $84.00 (20%)
ELM-IFNg-1
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Assay Format

Detection Range:
2.74 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1.3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01580
Gene ID:
  • 15978
Gene Symbols:
  • IFNG
Protein Name & Synonyms:
Interferon gamma (IFN-gamma)
Target Species:
  • Mouse

Target Background

Interferon-gamma (IFN-gamma) is a pleiotropic cytokine with antiproliferative, pro-apoptotic and antitumor activity. It is a homodimer formed by the association of two 17 kDa polypeptides, that bind in an antiparallel manner to form a mature 50 kDa molecule. This symmetry allows for a single molecule to bind two receptors amplifying downstream signaling. IFN-gamma is secreted by CD4 T helper type 1 cells and CD8 cytotoxic cells, γδ T cells, NK cells and to a lesser extent by NK T cells, B cells and APC cells. Its expression is induced by cytokines such as IL-12, IL-15, IL-18 and type I IFN. The effects of IFN-gamma are mediated through the activation of the JAK/STAT pathway which modulates the transcription of many genes. IFN-gamma signaling plays a role in host defense, immune surveillance, establishing adaptive immunity and in regulating inflammation, apoptosis and the cell cycle.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Lu, K., Liu, L., Xu, X. et al. ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis. J Neuroinflammation 17, 67 (2020). https://doi.org/10.1186/s12974-020-1713-z
    Species: Mouse
    Sample type: Tissue Lysate
  2. Jia H, Guo J, Wang P, Sun K, Chen J, Ren W, Wei T, Yang Y, Li J, Liu X, Li R, Zhong J, Wang M, Tian Z, Feng Z, Zhao T. A self-designed CpG ODN enhanced the anti-melanoma effect of pimozide. Int Immunopharmacol. 2020 Jun;83:106397. doi: 10.1016/j.intimp.2020.106397. Epub 2020 Mar 25. PMID: 32220805.
    Species: Mouse
    Sample type: Conditioned Media
  3. Drolia R, Amalaradjou MAR, Ryan V, Tenguria S, Liu D, Bai X, Xu L, Singh AK, Cox AD, Bernal-Crespo V, Schaber JA, Applegate BM, Vemulapalli R, Bhunia AK. Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nat Commun. 2020 Dec 11;11(1):6344. doi: 10.1038/s41467-020-20200-5. PMID: 33311493; PMCID: PMC7732855.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Luo YL, Liang LF, Gan YJ, Liu J, Zhang Y, Fan YN, Zhao G, Czarna A, Lu ZD, Du XJ, Shen S, Xu CF, Lian ZX, Wang J. An All-in-One Nanomedicine Consisting of CRISPR-Cas9 and an Autoantigen Peptide for Restoring Specific Immune Tolerance. ACS Appl Mater Interfaces. 2020 Oct 28;12(43):48259-48271. doi: 10.1021/acsami.0c10885. Epub 2020 Oct 19. PMID: 33070614.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Kim, S. H., Ryu, Y. C., Wang, H.-M. D., & Hwang, B. H. (2020). Optimally Fabricated Chitosan Particles Containing Ovalbumin Induced Cellular and Humoral Immunity in Immunized Mice. Biotechnology and Bioprocess Engineering. doi:10.1007/s12257-020-0004-y
    Species: Mouse
    Sample type: Serum
  6. Li S, Feng J, Wu F, Cai J, Zhang X, Wang H, Fetahu IS, Iwanicki I, Ma D, Hu T, Liu H. tet 2 promotes anti?tumor immunity by governing G?mdsc s and cd 8+ T?cell numbers. EMBO reports. 2020 Oct 5;21(10):e49425.
    Species: Mouse
    Sample type: Serum
  7. Chandan R., Banerjee R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep. 2018 Feb 8;8(1):2624. doi: 10.1038/s41598-018-21084-8.
    Species: Mouse
    Sample type: Conditioned Media
  8. Dey S., Bishayi B. Killing of S. aureus in murine peritoneal macrophages by Ascorbic acid along with antibiotics Chloramphenicol or Ofloxacin: Correlation with inflammation. Microb Pathog. 2018 Feb;115:239-250. doi: 10.1016/j.micpath.2017.12.048
    Species: Mouse
    Sample type: Conditioned Media
  9. Chandan, R., Banerjee, R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep 8, 2624 (2018). https://doi.org/10.1038/s41598-018-21084-9
    Species: Mouse
    Sample type: Conditioned media
  10. Nandi, Ajeya, and Biswadev Bishayi. "A novel CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1." Immunology letters 190 (2017): 93-107.
    Species: Mouse
    Sample type: Cell Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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