Mouse IFN-gamma ELISA

RayBio® Mouse IFN-gamma ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$1,608.00  
ELM-IFNg-CL-5
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Antigen Information

Accession Number:
  • P01580
Gene ID:
  • 15978
Gene Symbols:
  • IFNG
Protein Name & Synonyms:
Interferon gamma (IFN-gamma)
Target Species:
  • Mouse

Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kormann, Michael SD, et al. "Expression of therapeutic proteins after delivery of chemically modified mRNA in mice." Nature biotechnology 29.2 (2011): 154-157.
    Species: Mouse
    Sample type: Conditioned Media (Human and mouse alveolar type II epithelial cell lines, A549 and MLE15)
  2. Sun TS., Ju X., Gao HL., et al. Reciprocal functions of Cryptococcus neoformanscopper homeostasis machinery during pulmonaryinfection and meningoencephalitis.
    Species: Mouse
    Sample type: Tissue Lysate (Lung Tissue lysate from cryptococcus infection)
  3. Sun TS., Ju X., Gao HL., et al. Reciprocal functions of Cryptococcus neoformanscopper homeostasis machinery during pulmonaryinfection and meningoencephalitis.
    Species: Mouse
    Sample type: Tissue Lysate (Brain Tissue lysate from cryptococcus infection)
  4. Drolia R, Amalaradjou MAR, Ryan V, Tenguria S, Liu D, Bai X, Xu L, Singh AK, Cox AD, Bernal-Crespo V, Schaber JA, Applegate BM, Vemulapalli R, Bhunia AK. Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nat Commun. 2020 Dec 11;11(1):6344. doi: 10.1038/s41467-020-20200-5. PMID: 33311493; PMCID: PMC7732855.
    Species: Mouse
    Sample type: Tissue Lysate (ileal tissue lysates)
  5. Cheung, Wai W., et al. "A pegylated leptin antagonist ameliorates CKD-associated cachexia in mice." Journal of the American Society of Nephrology 25.1 (2014): 119-128.
    Species: Mice
    Sample type: Cell Lysate (Gastrocnemius muscle lysate )
  6. Luo YL, Liang LF, Gan YJ, Liu J, Zhang Y, Fan YN, Zhao G, Czarna A, Lu ZD, Du XJ, Shen S, Xu CF, Lian ZX, Wang J. An All-in-One Nanomedicine Consisting of CRISPR-Cas9 and an Autoantigen Peptide for Restoring Specific Immune Tolerance. ACS Appl Mater Interfaces. 2020 Oct 28;12(43):48259-48271. doi: 10.1021/acsami.0c10885. Epub 2020 Oct 19. PMID: 33070614.
    Species: Mouse
    Sample type: Tissue Lysate
  7. Mahanti, Sayantika, et al. "Increased resistance of immobilized-stressed mice to infection: correlation with behavioral alterations." Brain, behavior, and immunity 28 (2013): 115-127.
    Species: Mouse
    Sample type: Serum (immobilized-stressed mice infection susceptibility)
  8. Chandan R., Banerjee R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep. 2018 Feb 8;8(1):2624. doi: 10.1038/s41598-018-21084-8.
    Species: Mouse
    Sample type: Conditioned Media (RAW 264.7 macrophages treated with phosphatidylserine (PS)-based paclitaxel-liposomes-nanobubble conjugates)
  9. Chandan, R., Banerjee, R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep 8, 2624 (2018). https://doi.org/10.1038/s41598-018-21084-9
    Species: Mouse
    Sample type: Conditioned media (murine macrophage cells)
  10. Lu, K., Liu, L., Xu, X. et al. ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis. J Neuroinflammation 17, 67 (2020). https://doi.org/10.1186/s12974-020-1713-z
    Species: Mouse
    Sample type: Tissue Lysate (spinal cords)

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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