Mouse IL-1 beta ELISA

RayBio® Mouse IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,680.00  
ELM-IL1b-CL-5
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Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P10749
Gene ID:
  • 16176
Gene Symbols:
  • IL1B
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta)
Target Species:
  • Mouse

Target Background

Interleukin-1β (IL1b) is a pro-inflammatory cytokine important for immune responses to infection and injury. It is produced as a 31 kDa precursor, termed pro-IL-1b which is cleaved by caspase-1-dependent processing for secretion. IL1b is expressed in a wide variety of tissues and cells, especially in macrophages in the thymus, spleen, lymph nodes, Peyer's patches and bone marrow. Disruption of IL1b signaling can lead to inflammatory disease and malignancies.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wang P., Ji Y., Zhang X., et al. Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates. Nat Med. 2017 Apr;23(4):439-449. doi: 10.1038/nm.4290.
    Species: Mouse
    Sample type: Serum (steatohepatitis mouse model and role of casp8 in apoptosis)
  2. Ho P., Tsui Y., Feng X., et al. NF-?B-mediated degradation of the coactivator RIP140 regulates inflammatory responses and contributes to endotoxin tolerance. Nat Immunol. 2012 Mar 4;13(4):379-86. doi: 10.1038/ni.2238.
    Species: Mouse
    Sample type: Conditioned Media (Raw264.7 macrophages with LPS treatement)
  3. Huggenberger, Reto, et al. "An important role of lymphatic vessel activation in limiting acute inflammation." Blood 117.17 (2011): 4667-4678.
    Species: Mouse
    Sample type: Tissue lysate
  4. Wang X-A, Zhang R, Jiang D, et al. Interferon regulatory factor 9 protects against hepatic insulin resistance and steatosis in male mice. Hepatology. 2013 Aug;58(2):603-616.
    Species: Mouse
    Sample type: Serum
  5. Scanu, Anna, et al. "High-density lipoproteins inhibit urate crystal-induced inflammation in mice." Annals of the rheumatic diseases (2013): annrheumdis-2013.
    Species: Mouse
    Sample type: Air Pouch Exudates
  6. Weisser M., Demel U., Stein S., et al. Hyperinflammation in patients with chronic granulomatous disease leads to impairment of hematopoietic stem cell functions. J Allergy Clin Immunol. 2016 Feb 4. pii: S0091-6749(16)00031-2. doi: 10.1016/j.jaci.2015.11.028
    Species: Mouse
    Sample type: Bone Marrow Aspirate (Bone lavage samples of WT hind limbs mice)
  7. Cheung, Wai W., et al. "A pegylated leptin antagonist ameliorates CKD-associated cachexia in mice." Journal of the American Society of Nephrology 25.1 (2014): 119-128.
    Species: Mice
    Sample type: Cell Lysate (Gastrocnemius muscle lysate )
  8. Benito C, Tolón RM, Castillo AI, et al. ?—Amyloid exacerbates inflammation in astrocytes lacking fatty acid amide hydrolase through a mechanism involving PPAR-?, PPAR-? and TRPV1, but not CB1 or CB2 receptors. British Journal of Pharmacology 2012;166(4):1474-1489. doi:10.1111/j.1476-5381.2012.01889.x.
    Species: Mouse
    Sample type: Conditioned Media (Primary Neonatal astrocytes)
  9. Vazquez C., Tolon R., Grande M., et al. Endocannabinoid regulation of amyloid-induced neuroinflammation. Neurobiol Aging. 2015 Nov;36(11):3008-19. doi: 10.1016/j.neurobiolaging.2015.08.003.
    Species: Mouse
    Sample type: Tissue Lysate (Hippocampal lysate )
  10. Morganti JM, Nash KR, Grimmig BA, et al. The soluble isoform of CX3CL1 is necessary for neuroprotection in a mouse model of Parkinson’s disease. The Journal of neuroscience?: the official journal of the Society for Neuroscience 2012;32(42):14592-14601. doi:10.1523/JNEUROSCI.0539-12.2012.
    Species: Mouse
    Sample type: Tissue Lysate (Brain Lysate, ventral mesencephalon)
I measured the Mouse IL-1 beta (Cat#Ray Bio Mouse IL-1 Beta ELISA KIT) in Kidney tissue homogenates from mouse model of Diabetes (DB/DB-UNX) with Uni nephrectomy (UNX). I see the increased IL-1B levels in DB/DB-UNX compared to control Non-Diabetic (DB/M) mice. In this models we knew that there is increased inflammation together with Inflammasome signalling in the kidney of DB/DB-UNX. Therefore, I am interested in measure the IL-1B in our mouse of model of Diabetic kidney disease. This Kit from RayBiotech is working great in our mouse model and I would recommend this product.

Georgetown University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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