Mouse IL-1 beta ELISA

RayBio® Mouse IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,680.00  
ELM-IL1b-CL-5
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Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P10749
Gene ID:
  • 16176
Gene Symbols:
  • IL1B
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta)
Target Species:
  • Mouse

Target Background

Interleukin-1β (IL1b) is a pro-inflammatory cytokine important for immune responses to infection and injury. It is produced as a 31 kDa precursor, termed pro-IL-1b which is cleaved by caspase-1-dependent processing for secretion. IL1b is expressed in a wide variety of tissues and cells, especially in macrophages in the thymus, spleen, lymph nodes, Peyer's patches and bone marrow. Disruption of IL1b signaling can lead to inflammatory disease and malignancies.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Song, Jq., Jiang, Ly., Fu, Cp. et al. Heterozygous SOD2 deletion deteriorated chronic intermittent hypoxia-induced lung inflammation and vascular remodeling through mtROS-NLRP3 signaling pathway. Acta Pharmacol Sin 41, 1197–1207 (2020). https://doi.org/10.1038/s41401-019-0349-y
    Species: Mouse
    Sample type: Conditioned media
  2. Cheng, Q., Pan, J., Zhou, Zl. et al. Caspase-11/4 and gasdermin D-mediated pyroptosis contributes to podocyte injury in mouse diabetic nephropathy. Acta Pharmacol Sin (2020). https://doi.org/10.1038/s41401-020-00525-z
    Species: Mouse
    Sample type: Serum, Conditioned Media
  3. Lu, K., Liu, L., Xu, X. et al. ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis. J Neuroinflammation 17, 67 (2020). https://doi.org/10.1186/s12974-020-1713-z
    Species: Mouse
    Sample type: Tissue Lysate
  4. Liu N , Zhang GX , Niu YT , Wang Q , Zheng J , Yang JM , Sun T , Niu JG , Yu JQ . Anti-inflammatory and analgesic activities of indigo through regulating the IKK?/I?B/NF-?B pathway in mice. Food Funct. 2020 Oct 1;11(10):8537-8546. doi: 10.1039/c9fo02574j. Epub 2020 Oct 14. PMID: 33084638.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Xia H, Ge Y, Wang F, Ming Y, Wu Z, Wang J, Sun S, Huang S, Chen M, Xiao W, Yao S. Protectin DX ameliorates inflammation in sepsis-induced acute lung injury through mediating PPAR?/NF-?B pathway. Immunol Res. 2020 Oct;68(5):280-288. doi: 10.1007/s12026-020-09151-7. Epub 2020 Aug 26. PMID: 32845434.
    Species: Mouse
    Sample type: BAL Fluid
  6. Wang J, Wang B, Lv X, Wang L. NIK inhibitor impairs chronic periodontitis via suppressing non-canonical NF-?B and osteoclastogenesis. Pathog Dis. 2020 Oct 7;78(7):ftaa045. doi: 10.1093/femspd/ftaa045. PMID: 32860691.
    Species: Mouse
    Sample type: Conditioned Media
  7. Talley, S., Kalinina, O., Winek, M., Paik, W., Cannon, A. R., Alonzo, F., … Campbell, E. M. (2019). A Novel Caspase-1 Biosensor to Monitor the Progression of Inflammation In Vivo. The Journal of Immunology, ji1900619. doi:10.4049/jimmunol.1900619 
    Species: Mouse
    Sample type: Cell Lysate
  8. Aoki M, Murase T (2019) Obesity-associated insulin resistance adversely affects skin function. PLoS ONE 14(10): e0223528. https://doi.org/10.1371/journal.pone.0223528
    Species: Mouse
    Sample type: Serum
  9. Singh M, Kumari B, Yadav UCS. Regulation of oxidized LDL-induced inflammatory process through NLRP3 inflammasome activation by the deubiquitinating enzyme BRCC36. Inflamm Res. 2019 Dec;68(12):999-1010. doi: 10.1007/s00011-019-01281-5. Epub 2019 Sep 4. PMID: 31485755.
    Species: Mouse
    Sample type: Conditioned Media
  10. Han, Fei, et al. "Atorvastatin ameliorates LPS?induced inflammatory response by autophagy via AKT/mTOR signaling pathway." Journal of cellular biochemistry 119.2 (2018): 1604-1615.
    Species: Mouse
    Sample type: Conditioned Media
I measured the Mouse IL-1 beta (Cat#Ray Bio Mouse IL-1 Beta ELISA KIT) in Kidney tissue homogenates from mouse model of Diabetes (DB/DB-UNX) with Uni nephrectomy (UNX). I see the increased IL-1B levels in DB/DB-UNX compared to control Non-Diabetic (DB/M) mice. In this models we knew that there is increased inflammation together with Inflammasome signalling in the kidney of DB/DB-UNX. Therefore, I am interested in measure the IL-1B in our mouse of model of Diabetic kidney disease. This Kit from RayBiotech is working great in our mouse model and I would recommend this product.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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