Mouse IL-1 beta ELISA (For Lysates)

RayBio® Mouse IL-1 beta (IL-1 F2) ELISA Kit for cell and tissue lysate samples.

ELM-IL1b-CL-1
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Antigen Information

Accession Number:
  • P10749
Gene ID:
  • 16176
Gene Symbols:
  • IL1B
Target Species:
  • Mouse

Assay Format

Sensitivity:
5 pg/ml

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Specificity:
The antibody pair provided in this kit recognizes mouse IL-1 beta.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Lysates
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
  • Cell Lysis Buffer
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Nouri H., Karkhah A., Mohammadzadeh I., Sankian M. Elevated caspase?1 activity and IL?1? expression are associated with the IPAF inflammasome in an experimental model of allergy. Mol Med Rep. 2016 Apr;13(4):3356-62. doi: 10.3892/mmr.2016.4934.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Zhang S., Wang Y., Li D., et al. Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice. Medicines 2016, 3(3), 16; doi:10.3390/medicines3030016
    Species: Mouse
    Sample type: Tissue Lysate
  3. Fang Y., Yao L., Li C., et al. The blockage of the Nogo/NgR signal pathway in microglia alleviates the formation of A? plaques and tau phosphorylation in APP/PS1 transgenic mice. J Neuroinflammation. 2016; 13: 56. Published online 2016 Mar 3. doi: 10.1186/s12974-016-0522-x
    Species: Mouse
    Sample type: Tissue lysate
  4. Li H., Chen J., Qi Y., et al. Deficient PKR in RAX/PKR Association Ameliorates Ethanol-Induced Neurotoxicity in the Developing Cerebellum. The Cerebellum, Available January 2015. DOI 10.1007/s12311-015-0644-1
    Species: Mouse
    Sample type: Tissue Lysate
  5. Yu X., Yao JY., He J., Tian JW. Protection of MPTP-induced neuroinflammation and neurodegeneration by rotigotine-loaded microspheres. Life Sci. 2015 Mar 1;124:136-43. doi: 10.1016/j.lfs.2015.01.014
    Species: Mouse
    Sample type: Tissue Lysate
  6. Sun J., Wang F., Li H., et al. Neuroprotective Effect of Sodium Butyrate against Cerebral Ischemia/Reperfusion Injury in Mice. Biomed Res Int. 2015;2015:395895. doi: 10.1155/2015/395895.
    Species: Mouse
    Sample type: Tissue Lysate
  7. Rathore B., Jaggi K., Thakur S., et al. ANTI-INFLAMMATORY ACTIVITY OF CROCUS SATIVUS EXTRACT IN EXPERIMENTAL ARTHRITIS
    Species: Mouse
    Sample type: Tissue Lysate
  8. Vazquez C., Tolon R., Grande M., et al. Endocannabinoid regulation of amyloid-induced neuroinflammation. Neurobiol Aging. 2015 Nov;36(11):3008-19. doi: 10.1016/j.neurobiolaging.2015.08.003.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Zhang X., Zhou Y., Zhang J., et al. IL-35 inhibits acute graft-versus-host disease in a mouse model. Int Immunopharmacol. 2015 Dec;29(2):383-92. doi: 10.1016/j.intimp.2015.10.025
    Species: Mouse
    Sample type: Tissue Lysate
  10. Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314
    Species: Mouse
    Sample type: Tissue Lysate
It work equally perfect with fresh tumor protein lysates and with lysates after several freeze-thaw cycles, and gives consistent results. But standards are not as stable as they supposed to be: diluted standards didn't work after 3-day storage at -80C (standards for VEGFa kits works perfectly at the same time). But there are 2 vials of standards included, so it's not a problem actually.

Roswell Park Comprehensive Cancer Center
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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