Mouse IL-10 ELISA

RayBio® Mouse IL-10 ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELM-IL10-1
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Antigen Information

Accession Number:
  • P18893
Gene ID:
  • 16153
Gene Symbols:
  • IL-10
  • IL10
Protein Name & Synonyms:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Target Species:
  • Mouse

Assay Format

Detection Range:
45 pg/ml - 5000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
45 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Hsu, Tracy, and Samir Mitragotri. "Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer." Proceedings of the National Academy of Sciences 108.38 (2011): 15816-15821.
    Species: Mouse
    Sample type: Tissue Lysate (skin biopsy)
  2. Cheung, Wai W., et al. "A pegylated leptin antagonist ameliorates CKD-associated cachexia in mice." Journal of the American Society of Nephrology 25.1 (2014): 119-128.
    Species: Mice
    Sample type: Cell Lysate (Gastrocnemius muscle lysate )
  3. Luo YL, Liang LF, Gan YJ, Liu J, Zhang Y, Fan YN, Zhao G, Czarna A, Lu ZD, Du XJ, Shen S, Xu CF, Lian ZX, Wang J. An All-in-One Nanomedicine Consisting of CRISPR-Cas9 and an Autoantigen Peptide for Restoring Specific Immune Tolerance. ACS Appl Mater Interfaces. 2020 Oct 28;12(43):48259-48271. doi: 10.1021/acsami.0c10885. Epub 2020 Oct 19. PMID: 33070614.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Mahanti, Sayantika, et al. "Increased resistance of immobilized-stressed mice to infection: correlation with behavioral alterations." Brain, behavior, and immunity 28 (2013): 115-127.
    Species: Mouse
    Sample type: Serum (immobilized-stressed mice infection susceptibility)
  5. Shoulders H., Farner K., Singla D. Macrophage depletionbyclondronate attenuates bonemorphogeneticprotein-7 induced M2macrophagedifferentiationand improved systolicbloodvelocityin atherosclerosis. Translational Research Volume 203, January 2019, Pages 1-14, in press
    Species: Serum
    Sample type: Serum (macrophage polarization during blood cystolic celoctiy)
  6. Szpak D., Izem L., Verbovetskiy D., et al. ?M?2 Is Antiatherogenic in Female but Not Male Mice. J Immunol. 2018 Apr 1;200(7):2426-2438. doi: 10.4049/jimmunol.1700313.
    Species: Mouse
    Sample type: Conditioned Media (Macrophage polpulation studies in an atherosclerosis mouse model)
  7. Majhi A., Ashikary R., Bhattacharyya A., Mahanti S., Bishayi B. LEVOFLOXACIN AND CEFTRIAXONE IN COMBINATION ATTENUATES LUNG INFLAMMATION IN A MOUSE MODEL OF BACTEREMIC PNEUMONIA BY MULTI-DRUG RESISTANT Streptococcus pneumoniae VIA INHIBITION OF CYTOLYTIC ACTIVITIES OF PNEUMOLYSIN AND AUTOLYSIN. Antimicrob. Agents Chemother. Accepted ahead of Print, June 23 2014. doi:10.1128/AAC.03245-14
    Species: Mouse
    Sample type: Serum
  8. Wang L., Ren L., Tang T., et al. A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-?B and Caspase 3. Int J Nanomedicine. 2015; 10: 6725-6739.
    Species: Mouse
    Sample type: Conditioned Media (Raw264.7 macrophages with a novel copper nano material)
  9. Dey P., Ray S., Chaudhuri T. Immunomodulatory activities and phytochemical characterisation of the methanolic extract of Dioscorea alata aerial tuber. Journal of Functional Foods . Volume 23, May 2016, Pages 315-328. https://doi.org/10.1016/j.jff.2016.02.044
    Species: Mouse
    Sample type: Conditioned Media (Phagocytic activation of macrophages, and splenocyte culture)
  10. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Conditioned Media (Swiss Albino mice peritoneal macrophages)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.