Mouse IL-12 p40/70 ELISA

$398.00
ELM-IL12P40P70-1

RayBio® Mouse IL-12 p40/p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
5 pg/ml

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Detection Range
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma)
10 - 100 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Mouse
Protein Name & Synonyms
Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
Accession Number
Gene Names
Il12b
Gene ID
16160

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL12A
  • IL12B

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Adhikary R., Majhi A., Mahanti S., Bishayi B. Protective effects of methanolic extract of Adhatoda vasica Nees leaf in collagen-induced arthritis by modulation of synovial toll-like receptor-2 expression and release of pro-inflammatory mediators. Journal of Nutrition & Intermediary Metabolism. Volume 3, March 2016, Pages 1-11

  2. Adhikary R., Majhi A., Mahanti S., Bishayi B. Protective effects of methanolic extract of Adhatoda vasica Nees leaf in collagen-induced arthritis by modulation of synovial toll-like receptor-2 expression and release of pro-inflammatory mediators. Journal of Nutrition & Intermediary Metabolism. Volume 3, March 2016, Pages 1-11

  3. Spreadbury I., Ochoa-Cortes F., Ibeakanma C., et al. Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling. Neurogastroenterol Motil. 2015 Mar;27(3):347-55. doi: 10.1111/nmo.12497.
    Species: Mouse
    Sample Type: Conditioned Media

  4. Abo Elnazar S., Ghazy A., Ghonelm H., et al. Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes. Front Pharmacol. 2015; 6: 56. doi: 10.3389/fphar.2015.00056
    Species: Mouse
    Sample Type: Conditioned Media

  5. Ghazy A., Abu El-Nazar S., Ghoneim H., et al. Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes. Front Pharmacol. 2015; 6: 74. doi: 10.3389/fphar.2015.00074
    Species: Mouse
    Sample Type: Conditioned Media

  6. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample Type: Conditioned Media

  7. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample Type: Serum

  8. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample Type: Peritoneal Fluid

  9. Bishayi B., Bandyopadhyay D., Majhi A., Adhikary R. POSSIBLE ROLE OF TOLL LIKE RECEPTOR-2 (TLR-2) IN THE INTRACELLULAR SURVIVAL OF STAPHYLOCOCCUS AUREUS IN MURINE PERITONEAL MACROPHAGES: INVOLVEMENT OF CYTOKINES AND ANTI-OXIDANT ENZYMES. Scandinavian Journal of Immunology. 2014, May Epub Ahead of Print, doi:10.1111/sji.12195
    Species: Mouse
    Sample Type: Conditioned Media

  10. Bishayi B., et al. Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophagesand possible mechanisms of CCR-2/TLR-2 and MCP-1 signallingduring Staphylococcus aureus infection. Immunobiology Ahead of Print 2014, DOI:10.1016/j.imbio.2014.10.013
    Species: Mouse
    Sample Type: Conditioned Media

  11. Shanmugam A., Wang J., Markand S., et al. Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1γ, MIP2, MIP3α, and IL12 (p40/p70) by retinal Müller glial cells. J Neurochem. 2014 Dec 1. doi: 10.1111/jnc.13002
    Species: Mouse
    Sample Type: Conditioned Media

  12. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gsα-dependent signaling. Blood. 2013;121(6):930-939.
    Species: Mouse
    Sample Type: Conditioned Media

  13. Matsushita A., Seike M., Okawa H., et al. Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis. Exp Dermatol. 2012 Sep;21(9):714-5. doi: 10.1111/j.1600-0625.2012.01559.x.
    Species: Mouse
    Sample Type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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