Mouse IL-12 p40/70 ELISA

RayBio® Mouse IL-12 p40/p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Old price: $1,608.00
$1,286.40  
You save: $321.60 (20%)
ELM-IL12P40P70-5
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Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
10 - 100 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P43432
Gene ID:
  • 16160
Gene Symbols:
  • IL12B
Protein Name & Synonyms:
Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kormann, Michael SD, et al. "Expression of therapeutic proteins after delivery of chemically modified mRNA in mice." Nature biotechnology 29.2 (2011): 154-157.
    Species: Mouse
    Sample type: Conditioned Media (Human and mouse alveolar type II epithelial cell lines, A549 and MLE14)
  2. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling. Blood. 2013;121(6):930-939.
    Species: Mouse
    Sample type: Conditioned Media (osteocyte-enriched bone explants)
  3. Shanmugam A., Wang J., Markand S., et al. Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells. J Neurochem. 2014 Dec 1. doi: 10.1111/jnc.13002
    Species: Mouse
    Sample type: Conditioned Media (Muller cells incubated with LPS)
  4. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Conditioned Media (Swiss Albino mice peritoneal macrophages)
  5. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Serum (Swiss Albino mice peritoneal macrophages)
  6. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Peritoneal Fluid (Swiss Albino mice peritoneal macrophages, peritoneal fluid)
  7. Abo Elnazar S., Ghazy A., Ghonelm H., et al. Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes. Front Pharmacol. 2015; 6: 56. doi: 10.3389/fphar.2015.00056
    Species: Mouse
    Sample type: Conditioned Media (PBMCs stimulated with Con A and PWM alongside UVB irradiation)
  8. Ghazy A., Abu El-Nazar S., Ghoneim H., et al. Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes. Front Pharmacol. 2015; 6: 74. doi: 10.3389/fphar.2015.00074
    Species: Mouse
    Sample type: Conditioned Media (Splenocytes from gamma ray treated mice)
  9. Matsushita A., Seike M., Okawa H., et al. Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis. Exp Dermatol. 2012 Sep;21(9):714-5. doi: 10.1111/j.1600-0625.2012.01559.x.
    Species: Mouse
    Sample type: Tissue Lysate (Skin lysates from trinotriochlorobenzene TNCB treatment, allergic dermatitis model)
  10. Spreadbury I., Ochoa-Cortes F., Ibeakanma C., et al. Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling. Neurogastroenterol Motil. 2015 Mar;27(3):347-55. doi: 10.1111/nmo.12497.
    Species: Mouse
    Sample type: Conditioned Media (Soluble colonic mediators from isolated colon tissues)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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