Mouse IL-17A ELISA

RayBio® Mouse IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Old price: $723.60
$578.88  
You save: $144.72 (20%)
ELM-IL17-2
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Assay Format

Detection Range:
6 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 - 5 fold
Sensitivity:
6 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q62386
Gene ID:
  • 16171
Gene Symbols:
  • CTLA8
  • IL17
  • IL17A
Protein Name & Synonyms:
Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Luo YL, Liang LF, Gan YJ, Liu J, Zhang Y, Fan YN, Zhao G, Czarna A, Lu ZD, Du XJ, Shen S, Xu CF, Lian ZX, Wang J. An All-in-One Nanomedicine Consisting of CRISPR-Cas9 and an Autoantigen Peptide for Restoring Specific Immune Tolerance. ACS Appl Mater Interfaces. 2020 Oct 28;12(43):48259-48271. doi: 10.1021/acsami.0c10885. Epub 2020 Oct 19. PMID: 33070614.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Lu, K., Liu, L., Xu, X. et al. ADAMTS13 ameliorates inflammatory responses in experimental autoimmune encephalomyelitis. J Neuroinflammation 17, 67 (2020). https://doi.org/10.1186/s12974-020-1713-z
    Species: Mouse
    Sample type: Tissue Lysate (spinal cords)
  3. Nakai K., Yoneda K., Hosokawa Y., et al. Reduced expression of epidermal growth factor receptor, E-cadherin, and occludin in the skin of flaky tail mice is due to filaggrin and loricrin deficiencies. Am J Pathol. 2012 Sep;181(3):969-77. doi: 10.1016/j.ajpath.2012.06.005
    Species: Mouse
    Sample type: Serum
  4. Nakai K., Yoneda K., Hosokawa Y., et al. Reduced expression of epidermal growth factor receptor, E-cadherin, and occludin in the skin of flaky tail mice is due to filaggrin and loricrin deficiencies. Am J Pathol. 2012 Sep;181(3):969-77. doi: 10.1016/j.ajpath.2012.06.005
    Species: Mouse
    Sample type: Tissue Lysate (Skin Lysates)
  5. Trivedi PP., Jena GB., Tikoo KB., Kumar V. Melatonin Modulated Autophagy and Nrf2 Signaling Pathways in Mice With Colitis-Associated Colon Carcinogenesis. Molecular Carcinogenesis. Article first published online: 16 JAN 2015 | DOI: 10.1002/mc.22274
    Species: Mouse
    Sample type: Tissue Lysate (Colon tissue Lysates)
  6. Gao X., Yang H., Xu Y., et al. Iminosugar derivativeWGN-26 suppresses acute allograft rejection via inhibiting the IFN-?/p-STAT1/T-bet signaling pathway. nt Immunopharmacol. 2014 Dec;23(2):688-95.
    Species: Mouse
    Sample type: Serum (Normal and allo-skin transplants)
  7. Cai Y., Chen Z., Liu H., et al. Green tea epigallocatechin-3-gallate alleviates Porphyromonas gingivalis-induced periodontitis in mice. Int Immunopharmacol. 2015 Dec;29(2):839-45. doi: 10.1016/j.intimp.2015.08.033.
    Species: Mouse
    Sample type: Serum (Periodontitis mouse model treated with green tea extracts)
  8. Zhang X., Zhou Y., Zhang J., et al. IL-35 inhibits acute graft-versus-host disease in a mouse model. Int Immunopharmacol. 2015 Dec;29(2):383-92. doi: 10.1016/j.intimp.2015.10.025
    Species: Mouse
    Sample type: Tissue Lysate (Intestinal and liver lysate samples)
  9. Xuewa Guan, Yanjiao Lu, Guoqiang Wang, Peter Gibson, Fang Chen, Keyong Fang, Ziyan Wang, Zhiqiang Pang, Yingqiao Guo, Junying Lu, Yuze Yuan, Nan Ran, Fang Wang, "The Role of Regulatory T Cell in Nontypeable Haemophilus influenzae-Induced Acute Exacerbation of Chronic Obstructive Pulmonary Disease", Mediators of Inflammation, vol. 2018, Article ID 8387150, 14 pages, 2018. https://doi.org/10.1155/2018/8387150
    Species: Mouse
    Sample type: Serum
  10. Huang Q., Ma X., Zhu D., et al. Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice. J Neuroimmunol. 2015 Jul 15;284:67-73. doi: 10.1016/j.jneuroim.2015.05.009.
    Species: Mouse
    Sample type: Conditioned Media (Mouse splenocytes following treatment with a compount isolated from peony)
Mouse serum samples able to be diluted 5 fold and still generate strong signal. Fold change between two conditions seen on mouse array was validated using this ELISA kit. Easy instructions, great service.

Albert Einstein College of Medicine
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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