Mouse IL-17A ELISA

RayBio® Mouse IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • Q62386
Gene ID:
  • 16171
Gene Symbols:
  • CTLA8
  • IL17
  • IL17A
Protein Name & Synonyms:
Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
Target Species:
  • Mouse

Assay Format

Detection Range:
6 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 - 5 fold
6 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Trivedi PP., Jena GB., Tikoo KB., Kumar V. Melatonin Modulated Autophagy and Nrf2 Signaling Pathways in Mice With Colitis-Associated Colon Carcinogenesis. Molecular Carcinogenesis. Article first published online: 16 JAN 2015 | DOI: 10.1002/mc.22274
    Species: Mouse
    Sample type: Tissue Lysate
  2. Huang Q., Ma X., Zhu D., et al. Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice. J Neuroimmunol. 2015 Jul 15;284:67-73. doi: 10.1016/j.jneuroim.2015.05.009.
    Species: Mouse
    Sample type: Conditioned Media
  3. Cai Y., Chen Z., Liu H., et al. Green tea epigallocatechin-3-gallate alleviates Porphyromonas gingivalis-induced periodontitis in mice. Int Immunopharmacol. 2015 Dec;29(2):839-45. doi: 10.1016/j.intimp.2015.08.033.
    Species: Mouse
    Sample type: Serum
  4. Zhang X., Zhou Y., Zhang J., et al. IL-35 inhibits acute graft-versus-host disease in a mouse model. Int Immunopharmacol. 2015 Dec;29(2):383-92. doi: 10.1016/j.intimp.2015.10.025
    Species: Mouse
    Sample type: Tissue Lysate
  5. Gao X., Yang H., Xu Y., et al. Iminosugar derivativeWGN-26 suppresses acute allograft rejection via inhibiting the IFN-?/p-STAT1/T-bet signaling pathway. nt Immunopharmacol. 2014 Dec;23(2):688-95.
    Species: Mouse
    Sample type: Serum
  6. Matsushita A., et al. Close relationship between T helper (Th)17 and Th2 response in murine allergic contact dermatitis. Clinical and Experimental Dermatology, epub ahead of print. 5 SEP 2014. DOI: 10.1111/ced.12425
    Species: Mouse
    Sample type: Tissue Lysate
  7. Trivedi, P. P., and G. B. Jena. "Melatonin reduces ulcerative colitis-associated local and systemic damage in mice: investigation on possible mechanisms."?Digestive diseases and sciences?58.12 (2013): 3460-3474.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Nakai K., Yoneda K., Hosokawa Y., et al. Reduced expression of epidermal growth factor receptor, E-cadherin, and occludin in the skin of flaky tail mice is due to filaggrin and loricrin deficiencies. Am J Pathol. 2012 Sep;181(3):969-77. doi: 10.1016/j.ajpath.2012.06.005
    Species: Mouse
    Sample type: Tissue Lysate

No posts found

Write A Review
U.S. Non-U.S.


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Recently Viewed Recently Viewed

Customers Also Bought Customers Also Bought