Mouse IL-2 ELISA

RayBio® Mouse IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
1 pg/ml - 40 pg/ml
Recommended Dilution (serum & plasma):
2 fold
1 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P04351
Gene ID:
  • 16183
Gene Symbols:
  • IL-2
  • IL2
Protein Name & Synonyms:
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Rodrigues MA., Figueiredo L., Padrela L., et al. Development of a novel mucosal vaccine against strangles by supercritical enhanced atomization spray-drying of Streptococcus equi extracts and evaluation in a mouse model. Eur J Pharm Biopharm. 2012 Oct;82(2):392-400. doi: 10.1016/j.ejpb.2012.07.005.
    Species: Mouse
    Sample type: Conditioned Media (Splenocytes stimulated with SeM)
  2. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v
    Species: Mouse
    Sample type: Serum
  3. Tian X., et al. Toxicity evaluation of [email protected] nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo. International Journal of Nanomedicine. 2014 Aug 21;9:4043-53. doi: 10.2147/IJN.S66164.
    Species: Mouse
    Sample type: Serum
  4. Dey P., Ray S., Chaudhuri T. Immunomodulatory activities and phytochemical characterisation of the methanolic extract of Dioscorea alata aerial tuber. Journal of Functional Foods . Volume 23, May 2016, Pages 315-328.
    Species: Mouse
    Sample type: Conditioned Media (Phagocytic activation of macrophages, and splenocyte culture)
  5. Bizargity P, Liu K, Wang L, Hancock WW, Visner GA. Inhibitory Effects of Pirfenidone on Dendritic Cells and Lung Allograft Rejection. Transplantation. 2012;94(2):114-122. doi:10.1097/TP.0b013e3182584879.
    Species: Mouse
    Sample type: Cell Lysate (Lipopolysaccharide and allogeneic treated mice bone marrow DC)
  6. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-InducedAcute Lung Inflammation Through Inhibition of NF-?b, STAT3 and Pro-Inflammatory Mediators and Enhancement of I?B? and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y
    Species: Mouse
    Sample type: Pleural Fluid (Pleural Fluid)
  7. Figueiredo L., Cadete A., Goncalves L., et al. Intranasal immunisation of mice against Streptococcus equi using positively charged nanoparticulate carrier systems. Vaccine. 2012 Oct 12;30(46):6551-8. doi: 10.1016/j.vaccine.2012.08.050.
    Species: Mouse
    Sample type: Conditioned Media (Splenic T cells incubated with Streptococcus equi M protein (immunization protocol)
  8. Li W., Li M., Deng G., et al. Prime-boost vaccination with Bacillus Calmette Guerin and a recombinant adenovirus co-expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. Mol Med Rep. 2015 Aug;12(2):3073-80. doi: 10.3892/mmr.2015.3770
    Species: Mouse
    Sample type: Conditioned Media (Vaccination models against TB)
  9. Sun A, Wei H, Sun R, Xiao W, Yang Y, Tian Z. Human Interleukin-15 Improves Engraftment of Human T Cells in NOD-SCID mice. Clin Vaccine Immunol. 2006;13(2):227-234.
    Species: Mouse
    Sample type: Conditioned Media (Mouse Lymphoid cell supernate)
  10. Tian X., et al. In Vivo Immunotoxicity of [email protected](Y0.5Gd0.45Eu0.05)2O3 as Dual-Modality Nanoprobes. Int. J. Mol. Sci. 2014, 15, 13649-13662; doi:10.3390/ijms150813649
    Species: Mouse
    Sample type: Serum

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