Mouse IL-4 ELISA

RayBio® Mouse IL-4 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Old price: $402.00
$321.60  
You save: $80.40 (20%)
ELM-IL4-CL-1
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Assay Format

Detection Range:
0.6 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
0.6 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P07750
Gene ID:
  • 16189
Gene Symbols:
  • IL-4
  • IL4
Protein Name & Synonyms:
Interleukin-4 (IL-4) (B-cell IgG differentiation factor) (B-cell growth factor 1) (B-cell stimulatory factor 1) (BSF-1) (IGG1 induction factor) (Lymphocyte stimulatory factor 1)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Fang Y., Yao L., Li C., et al. The blockage of the Nogo/NgR signal pathway in microglia alleviates the formation of A? plaques and tau phosphorylation in APP/PS1 transgenic mice. J Neuroinflammation. 2016; 13: 56. Published online 2016 Mar 3. doi: 10.1186/s12974-016-0522-x
    Species: Mouse
    Sample type: Tissue lysate (brain tissue lysates in Ab plaques)
  2. Rodrigues MA., Figueiredo L., Padrela L., et al. Development of a novel mucosal vaccine against strangles by supercritical enhanced atomization spray-drying of Streptococcus equi extracts and evaluation in a mouse model. Eur J Pharm Biopharm. 2012 Oct;82(2):392-400. doi: 10.1016/j.ejpb.2012.07.005.
    Species: Mouse
    Sample type: Conditioned Media (Splenocytes stimulated with SeM)
  3. Xiao G, Li X, Kumar A, Cui Z. Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses. European journal of pharmaceutics and biopharmaceutics?: official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV. 2012;82(1):212-217. doi:10.1016/j.ejpb.2012.06.012.
    Species: Mouse
    Sample type: Conditioned Media (Splenocytes treated with beta-galactosidase)
  4. Jian Y., Zou Y., Chen S., Zhu C., Wu A., Liu Y., Ma L., et al. The anti-inflammatory effect of donepezil on experimental autoimmune encephalomyelitis in C57 BL/6 mice. Neuropharmacology 73 (2013) 415-424
    Species: Mouse
    Sample type: Conditioned Media (Splenocytes)
  5. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v
    Species: Mouse
    Sample type: Serum
  6. Tian X., et al. Toxicity evaluation of [email protected] nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo. International Journal of Nanomedicine. 2014 Aug 21;9:4043-53. doi: 10.2147/IJN.S66164.
    Species: Mouse
    Sample type: Serum
  7. Dey P., Ray S., Chaudhuri T. Immunomodulatory activities and phytochemical characterisation of the methanolic extract of Dioscorea alata aerial tuber. Journal of Functional Foods . Volume 23, May 2016, Pages 315-328. https://doi.org/10.1016/j.jff.2016.02.044
    Species: Mouse
    Sample type: Conditioned Media (Phagocytic activation of macrophages, and splenocyte culture)
  8. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Conditioned Media (Swiss Albino mice peritoneal macrophages)
  9. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Serum (Swiss Albino mice peritoneal macrophages)
  10. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Peritoneal Fluid (Swiss Albino mice peritoneal macrophages, peritoneal fluid)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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