Mouse IL-4 ELISA

RayBio® Mouse IL-4 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P07750
Gene ID:
  • 16189
Gene Symbols:
  • IL-4
  • IL4
Protein Name & Synonyms:
Interleukin-4 (IL-4) (B-cell IgG differentiation factor) (B-cell growth factor 1) (B-cell stimulatory factor 1) (BSF-1) (IGG1 induction factor) (Lymphocyte stimulatory factor 1)
Target Species:
  • Mouse

Assay Format

Detection Range:
0.6 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
2 fold
0.6 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Fang Y., Yao L., Li C., et al. The blockage of the Nogo/NgR signal pathway in microglia alleviates the formation of A? plaques and tau phosphorylation in APP/PS1 transgenic mice. J Neuroinflammation. 2016; 13: 56. Published online 2016 Mar 3. doi: 10.1186/s12974-016-0522-x
    Species: Mouse
    Sample type: Tissue lysate
  2. Dey P., Ray S., Chaudhuri T. Immunomodulatory activities and phytochemical characterisation of the methanolic extract of Dioscorea alata aerial tuber. Journal of Functional Foods . Volume 23, May 2016, Pages 315-328.
    Species: Mouse
    Sample type: Conditioned Media
  3. Dey P., Chaudhuri TK. Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/T H2 cytokine balance in murine splenic lymphocytes. Cytotechnology. 2015 Feb 14. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  4. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample type: Serum
  5. Ghosh S., Mukherjee S., Choudhury S., et al. Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine. Cell Signal. 2015 Jul;27(7):1398-412. doi: 10.1016/j.cellsig.2015.03.013.
    Species: Mouse
    Sample type: Peritoneal Fluid
  6. Huang Q., Ma X., Zhu D., et al. Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice. J Neuroimmunol. 2015 Jul 15;284:67-73. doi: 10.1016/j.jneuroim.2015.05.009.
    Species: Mouse
    Sample type: Conditioned Media
  7. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-InducedAcute Lung Inflammation Through Inhibition of NF-?b, STAT3 and Pro-Inflammatory Mediators and Enhancement of I?B? and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y
    Species: Mouse
    Sample type: Pleural Fluid
  8. Gu X., Zie Y., Wang S., Peng X., Lai S., Yang G. Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice. Exp Appl Acarol. 2014 Apr 13. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  9. Kawahara, K. Cooperative Therapeutic Action of Retinoic Acid Receptor and Retinoid X Receptor Agonists in a Mouse Model of Alzheimer's Disease. Journal of Alzheimer's Disease 2014. Epub Ahead of Print. DOI: 10.3233/JAD-132720
    Species: Mouse
    Sample type: Tissue Lysate
  10. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v
    Species: Mouse
    Sample type: Serum

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