Mouse IL-6 ELISA

RayBio® Mouse IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELM-IL6-1
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Antigen Information

Accession Number:
  • P08505
Gene ID:
  • 16193
Gene Symbols:
  • IL-6
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6) (B-cell hybridoma growth factor) (Interleukin HP-1)
Target Species:
  • Mouse

Assay Format

Detection Range:
2 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Yuan D., Huang S., Berger E., et al. Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS. Cancer Cell . 2017 Jun 12;31(6):771-789.e6. doi: 10.1016/j.ccell.2017.05.006.
    Species: Mouse
    Sample type: Tissue Lysate (Liver homogenates)
  2. Wu J.B., Yin L., Shi C., et al. MAOA-Dependent Activation of Shh-IL6-RANKL Signaling Network Promotes Prostate Cancer Metastasis by Engaging Tumor-Stromal Cell Interactions. Cancer Cell . 2017 Mar 13;31(3):368-382. doi: 10.1016/j.ccell.2017.02.003.
    Species: Mouse
    Sample type: Conditioned Media (monoamine oxidase A (MAOA) in cancer signaling)
  3. Kumar M., Sahu S., Kumar R., et al. MicroRNA let-7 Modulates the Immune Response to Mycobacterium tuberculosis Infection via Control of A20, an Inhibitor of the NF-kB Pathway. Cell Host Microbe. 2015 Mar 11;17(3):345-56. doi: 10.1016/j.chom.2015.01.007.
    Species: Mouse
    Sample type: Conditioned Media (A20-/- macrophages)
  4. Liu Z., Reba S., Chen W., P S., Boom W., et al. Regulation of mammalian siderophore 2,5-DHBA in the innate immune response to infection. Journal of Experimental Medicine. vol. 211 no. 6 1197-1213
    Species: Mouse
    Sample type: Conditioned Media (RAW cells)
  5. Drolia R, Amalaradjou MAR, Ryan V, Tenguria S, Liu D, Bai X, Xu L, Singh AK, Cox AD, Bernal-Crespo V, Schaber JA, Applegate BM, Vemulapalli R, Bhunia AK. Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nat Commun. 2020 Dec 11;11(1):6344. doi: 10.1038/s41467-020-20200-5. PMID: 33311493; PMCID: PMC7732855.
    Species: Mouse
    Sample type: Tissue Lysate (ileal tissue lysates)
  6. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Mouse
    Sample type: Conditioned Media (Human UMUC3 coculture)
  7. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Mouse
    Sample type: Tissue Lysate (Tumor lysate from lungs of baldder cancer)
  8. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Conditioned Media (cancer-associated (CAF) and normal fibroblasts)
  9. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Tissue Lysate (bladder)
  10. Cheung, Wai W., et al. "A pegylated leptin antagonist ameliorates CKD-associated cachexia in mice." Journal of the American Society of Nephrology 25.1 (2014): 119-128.
    Species: Mice
    Sample type: Cell Lysate (Gastrocnemius muscle lysate )
Elisa Mouse IL-6, I tested some plasma samples, and I was not able to detect any IL-6 even after incubating overnight at 4C

University of Michigan
I used this kit to quantify IL-6 levels in whole brain lysates. Protocol is clear and easy, the assay works well

Cold Spring Harbor Laboratory
I analyzed mouse plasma and brain tissue with this ELISA. The directions were clear and easy to follow. No problems were noted. Would use again!

University of Mary Washington
I have used this Elisa kit, its quite affordable considering the amount of time saved and data generated from it. I have even compared IL-6 ELISA data to IL-6 luminex data (from the same samples) and they are relatively similar.

NanoBST
I used this kit with LPS-inflammed RAW264.7 cells supernatants. It was very sensitive! The instructions were clear and detailed and the kit well assembled. I also liked that you can use just half a plate and keep the other strips for another time. I would strongly recommend.

University of Arkansas
I used this ELISA kit to measure IL-6 in mouse serum. While this was not the easiest ELISA to perform, the instructions were simple enough to follow.

Emory University
Protocol was very easy to follow and very reliable. Cost effective kit!

NanoBST
I tested mouse serum. I did not measure any signals.

FDA
The kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.

Houston Methodist Research Institute
I used this kit to analyze mouse serum samples and it did exactly what it is supposed to do. I highly recommend this product!

BTS Research
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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