Mouse IL-6 ELISA

RayBio® Mouse IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELM-IL6-1
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Antigen Information

Accession Number:
  • P08505
Gene ID:
  • 16193
Gene Symbols:
  • IL-6
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6) (B-cell hybridoma growth factor) (Interleukin HP-1)
Target Species:
  • Mouse

Assay Format

Detection Range:
2 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Shoulders H., Farner K., Singla D. Macrophage depletion by clondronate attenuates bone morphogenetic protein-7 induced M2 macrophage differentiation and improved systolic blood velocity in atherosclerosis. Translational ResearchVolume 203, January 2019, Pages 1-14, in press
    Species: Serum
    Sample type: Serum
  2. Chandan R., Banerjee R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep. 2018 Feb 8;8(1):2624. doi: 10.1038/s41598-018-21084-8.
    Species: Mouse
    Sample type: Conditioned Media
  3. Szpak D., Izem L., Verbovetskiy D., et al. ?M?2 Is Antiatherogenic in Female but Not Male Mice. J Immunol. 2018 Apr 1;200(7):2426-2438. doi: 10.4049/jimmunol.1700313.
    Species: Mouse
    Sample type: Conditioned Media
  4. Mendt M., Kamerkar S., Sugimoto H., et al. Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight. 2018 Apr 19;3(8). pii: 99263. doi: 10.1172/jci.insight.99263.
    Species: Mouse
    Sample type: Serum
  5. Dey S., Bishayi B. Killing of S. aureus in murine peritoneal macrophages by Ascorbic acid along with antibiotics Chloramphenicol or Ofloxacin: Correlation with inflammation. Microb Pathog. 2018 Feb;115:239-250. doi: 10.1016/j.micpath.2017.12.048
    Species: Mouse
    Sample type: Conditioned Media
  6. Allen, London, et al. "Effects of delta-tocotrienol on obesity-related adipocyte hypertrophy, inflammation and hepatic steatosis in high-fat-fed mice."?The Journal of nutritional biochemistry?48 (2017): 128-137.
    Species: Mouse
    Sample type: Tissue
  7. Nandi, Ajeya, and Biswadev Bishayi. "A novel CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1."?Immunology letters?190 (2017): 93-107.
    Species: Mouse
    Sample type: Cell lysates
  8. Bil-Lula I., Krzywonos-Zawadzka A., Sawicki G., Wozniak M. An infection of human adenovirus 31 affects the differentiation of preadipocytes into fat cells, its metabolic profile and fat accumulation. J Med Virol. 2016 Mar;88(3):400-7. doi: 10.1002/jmv.24362.
    Species: Mouse
    Sample type: Conditioned Media
  9. Thomas J., Khanam R., Vohora D. Augmentation of effect of venlafaxine by folic acid in behavioral paradigms of depression in mice: Evidence of serotonergic and pro-inflammatory cytokine pathways. Pharmacol Rep. 2016 Apr;68(2):396-403. doi: 10.1016/j.pharep.2015.10.003.
    Species: Mouse
    Sample type: Serum
  10. Yu J., Yuan X., Liu Y., et al. Delayed Administration of WP1066, an STAT3 Inhibitor, Ameliorates Radiation-Induced Lung Injury in Mice. Lung (2016) 194: 67. doi:10.1007/s00408-015-9821-8
    Species: Mouse
    Sample type: Serum
I have used this Elisa kit, its quite affordable considering the amount of time saved and data generated from it. I have even compared IL-6 ELISA data to IL-6 luminex data (from the same samples) and they are relatively similar.

NanoBST
I used this kit with LPS-inflammed RAW264.7 cells supernatants. It was very sensitive! The instructions were clear and detailed and the kit well assembled. I also liked that you can use just half a plate and keep the other strips for another time. I would strongly recommend.

University of Arkansas
I used this ELISA kit to measure IL-6 in mouse serum. While this was not the easiest ELISA to perform, the instructions were simple enough to follow.

Emory University
Protocol was very easy to follow and very reliable. Cost effective kit!

NanoBST
I tested mouse serum. I did not measure any signals.

FDA
The kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.

Houston Methodist Research Institute
I used this kit to analyze mouse serum samples and it did exactly what it is supposed to do. I highly recommend this product!

BTS Research
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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