Mouse IL-6 ELISA

RayBio® Mouse IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$420.00  
ELM-IL6-1
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Assay Format

Detection Range:
2 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P08505
Gene ID:
  • 16193
Gene Symbols:
  • IL-6
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6) (B-cell hybridoma growth factor) (Interleukin HP-1)
Target Species:
  • Mouse

Target Background

Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-α and IL-1, and activation of IL-1Rα and IL-10.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Romacho T, Valencia I, Ramos-González M, Vallejo S, López-Esteban M, Lorenzo O, Cannata P, Romero A, San Hipólito-Luengo A, Gómez-Cerezo JF, Peiró C, Sánchez-Ferrer CF. Visfatin/eNampt induces endothelial dysfunction in vivo: a role for Toll-Like Receptor 4 and NLRP3 inflammasome. Sci Rep. 2020 Mar 25;10(1):5386. doi: 10.1038/s41598-020-62190-w. PMID: 32214150; PMCID: PMC7096459.
    Species: Mouse
    Sample type: Conditioned media
  2. Liu N , Zhang GX , Niu YT , Wang Q , Zheng J , Yang JM , Sun T , Niu JG , Yu JQ . Anti-inflammatory and analgesic activities of indigo through regulating the IKK?/I?B/NF-?B pathway in mice. Food Funct. 2020 Oct 1;11(10):8537-8546. doi: 10.1039/c9fo02574j. Epub 2020 Oct 14. PMID: 33084638.
    Species: Mouse
    Sample type: Tissue Lysate
  3. Drolia R, Amalaradjou MAR, Ryan V, Tenguria S, Liu D, Bai X, Xu L, Singh AK, Cox AD, Bernal-Crespo V, Schaber JA, Applegate BM, Vemulapalli R, Bhunia AK. Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nat Commun. 2020 Dec 11;11(1):6344. doi: 10.1038/s41467-020-20200-5. PMID: 33311493; PMCID: PMC7732855.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Sellmayr M, Hernandez Petzsche MR, Ma Q, Krüger N, Liapis H, Brink A, Lenz B, Angelotti ML, Gnemmi V, Kuppe C, Kim H, Bindels EMJ, Tajti F, Saez-Rodriguez J, Lech M, Kramann R, Romagnani P, Anders HJ, Steiger S. Only Hyperuricemia with Crystalluria, but not Asymptomatic Hyperuricemia, Drives Progression of Chronic Kidney Disease. J Am Soc Nephrol. 2020 Dec;31(12):2773-2792. doi: 10.1681/ASN.2020040523. Epub 2020 Sep 16. PMID: 32938648.
    Species: Mouse
    Sample type: Serum
  5. Luo YL, Liang LF, Gan YJ, Liu J, Zhang Y, Fan YN, Zhao G, Czarna A, Lu ZD, Du XJ, Shen S, Xu CF, Lian ZX, Wang J. An All-in-One Nanomedicine Consisting of CRISPR-Cas9 and an Autoantigen Peptide for Restoring Specific Immune Tolerance. ACS Appl Mater Interfaces. 2020 Oct 28;12(43):48259-48271. doi: 10.1021/acsami.0c10885. Epub 2020 Oct 19. PMID: 33070614.
    Species: Mouse
    Sample type: Tissue Lysate
  6. Gu I, Brownmiller C, Stebbins NB, Mauromoustakos A, Howard L, Lee SO. Berry Phenolic and Volatile Extracts Inhibit Pro-Inflammatory Cytokine Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-?B Signaling Pathway. Antioxidants (Basel). 2020 Sep 15;9(9):871. doi: 10.3390/antiox9090871. PMID: 32942640; PMCID: PMC7554842.
    Species: Mouse
    Sample type: Conditioned Media
  7. Sharma P, Kulkarni GT, Sharma B. Possible involvement of D2/D3 receptor activation in ischemic preconditioning mediated protection of the brain. Brain Res. 2020 Dec 1;1748:147116. doi: 10.1016/j.brainres.2020.147116. Epub 2020 Sep 11. PMID: 32919985.
    Species: Mouse
    Sample type: Conditioned Media
  8. Xia H, Ge Y, Wang F, Ming Y, Wu Z, Wang J, Sun S, Huang S, Chen M, Xiao W, Yao S. Protectin DX ameliorates inflammation in sepsis-induced acute lung injury through mediating PPAR?/NF-?B pathway. Immunol Res. 2020 Oct;68(5):280-288. doi: 10.1007/s12026-020-09151-7. Epub 2020 Aug 26. PMID: 32845434.
    Species: Mouse
    Sample type: BAL Fluid
  9. Wang J, Wang B, Lv X, Wang L. NIK inhibitor impairs chronic periodontitis via suppressing non-canonical NF-?B and osteoclastogenesis. Pathog Dis. 2020 Oct 7;78(7):ftaa045. doi: 10.1093/femspd/ftaa045. PMID: 32860691.
    Species: Mouse
    Sample type: Conditioned Media
  10. https://www.embopress.org/doi/full/10.15252/embr.201949425
    Species: mouse
    Sample type: Serum
Very simple kit to use, tricky with mouse plasma samples though

BCM
Elisa Mouse IL-6, I tested some plasma samples, and I was not able to detect any IL-6 even after incubating overnight at 4C

University of Michigan
I used this kit to quantify IL-6 levels in whole brain lysates. Protocol is clear and easy, the assay works well

Cold Spring Harbor Laboratory
I analyzed mouse plasma and brain tissue with this ELISA. The directions were clear and easy to follow. No problems were noted. Would use again!

University of Mary Washington
I have used this Elisa kit, its quite affordable considering the amount of time saved and data generated from it. I have even compared IL-6 ELISA data to IL-6 luminex data (from the same samples) and they are relatively similar.

NanoBST
I used this kit with LPS-inflammed RAW264.7 cells supernatants. It was very sensitive! The instructions were clear and detailed and the kit well assembled. I also liked that you can use just half a plate and keep the other strips for another time. I would strongly recommend.

University of Arkansas
I used this ELISA kit to measure IL-6 in mouse serum. While this was not the easiest ELISA to perform, the instructions were simple enough to follow.

Emory University
Protocol was very easy to follow and very reliable. Cost effective kit!

NanoBST
I tested mouse serum. I did not measure any signals.

FDA
The kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.

Houston Methodist Research Institute
I used this kit to analyze mouse serum samples and it did exactly what it is supposed to do. I highly recommend this product!

BTS Research
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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