Mouse Inflammation Array G1

RayBio® G-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.

$540.35
AAM-INF-G1-4
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
Lymphotactin
(XCL1)
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TIMP-2
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Blackburn N., Sofrenovic T., Kuratis D., et al. Timing underpins the benefits associated with injectable collagen biomaterial therapy for the treatment of myocardial infarction. Biomaterials 39 (2015) 182e192
    Species: Mouse
    Sample type: Tissue Lysate
  2. Liu X., Wu J., Wang M., et al. Identification of Novel Inflammatory Cytokines and Contribution of Keratinocyte-Derived Chemokine to Inflammation in Response to Vibrio vulnificus Infection in Mice. Inflammation. 2015 Oct;38(5):1864-73. doi: 10.1007/s10753-015-0166-5.
    Species: Mouse
    Sample type: Tissue Lysate
  3. Velsko I., Chukkapalli S., Rivera-Kweh M., et al. Fusobacterium nucleatum Alters Atherosclerosis Risk Factors and Enhances Inflammatory Markers with an Atheroprotective Immune Response in ApoE(null) Mice. PLoS One. 2015 Jun 16;10(6):e0129795. doi: 10.1371/journal.pone.0129795.
    Species: Mouse
    Sample type: Serum
  4. Zhou Y., Yu X., Chen H., et al. Leptin Deficiency Shifts Mast Cells toward Anti-Inflammatory Actions and Protects Mice from Obesity and Diabetes by Polarizing M2 Macrophages. Cell Metab. 2015 Dec 1;22(6):1045-58. doi: 10.1016/j.cmet.2015.09.013.
    Species: Mouse
    Sample type: Conditioned Media
  5. Huang Y., et al. Met-CCL5 represents an immunotherapy strategy to ameliorate rabies virus infection. Huang et al. Journal of Neuroinflammation 2014, 11:146. doi:10.1186/s12974-014-0146-y
    Species: Mouse
    Sample type: Tissue Lysate
  6. Smith MA., Michael R., Aravindan RG., et al. Anatase titanium dioxide nanoparticles in mice: evidence for induced structural and functional sperm defects after short-, but not long-, term exposure. Asian J Androl. 2014 Oct 24. doi: 10.4103/1008-682X.143247
    Species: Mouse
    Sample type: Serum
  7. Velsko I., Chukkapalli S., Rivera M., Lee J., Chen H., Zhenge D., et al. Active Invasion of Oral and Aortic Tissues by Porphyromonas gingivalis in Mice Causally Links Periodontitis and Atherosclerosis. PLOS One (2014)
    Species: Mouse
    Sample type: Serum
  8. Coulombe G, Leblanc C, Cagnol S, et al. Epithelial Tyrosine Phosphatase SHP-2 Protects against Intestinal Inflammation in Mice. Mol Cell Biol. 2013;33(11):2275-2284.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Wang Y., Yang Y., Liu X., et al. Inhibition of clathrin/dynamin-dependent internalization interferes with LPS-mediated TRAM-TRIF-dependent signaling pathway. Cell Immunol. 2012;274(1-2):121-9. doi: 10.1016/j.cellimm.2011.12.007.
    Species: Mouse
    Sample type: Conditioned Media
  10. Chopy, Damien, et al. "Ambivalent role of the innate immune response in rabies virus pathogenesis." Journal of virology 85.13 (2011): 6657-6668.
    Species: Mouse
    Sample type: Tissue Lysate

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Total list price $656.50
Price for all $575.19

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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