Mouse Insulin ELISA

RayBio® Mouse Insulin ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$723.60  
ELM-Insulin-2
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Assay Format

Detection Range:
5 uIU/ml - 400 uIU/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 mIU/ml

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Specificity:
This ELISA antibody pair detects mouse, human, rat, porcine and bovine Insulin.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01325
Gene ID:
  • 16333
Gene Symbols:
  • INS-1
  • INS1
Protein Name & Synonyms:
Insulin-1 [Cleaved into: Insulin-1 B chain Insulin-1 A chain]
Target Species:
  • Mouse

Target Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Uday Chandrika, K., Tripathi, R., Kameshwari, Y. et al. Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas. Tissue Eng Regen Med 18, 99–112 (2021). https://doi.org/10.1007/s13770-020-00296-y
    Species: Mouse
    Sample type: Serum
  2. Liu Z, Ding Z, Lynch EC, McCauley N, Zhou Y, Zhang KK, Xie L. Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic ?-cell function in male offspring induced by maternal high-fat diet. J Nutr Biochem. 2020 Dec;86:108495. doi: 10.1016/j.jnutbio.2020.108495. Epub 2020 Sep 16. PMID: 32949717; PMCID: PMC7643480.
    Species: Mouse
    Sample type: Plasma
  3. Arha, Deepti, et al. "Isoalantolactone derivative promotes glucose utilization in skeletal muscle cells and increases energy expenditure in db/db mice via activating AMPK-dependent signaling." Molecular and cellular endocrinology 460 (2018): 134-151.
    Species: Mouse
    Sample type: Cell Lysate (rat skeletal muscle cells)
  4. Zhou Y, Liu Z, Lynch EC, He L, Cheng H, Liu L, Li Z, Li J, Lawless L, Zhang KK, Xie L. Osr1 regulates hepatic inflammation and cell survival in the progression of non-alcoholic fatty liver disease. Lab Invest. 2020 Oct 1. doi: 10.1038/s41374-020-00493-2. Epub ahead of print. PMID: 33005011.
    Species: Mouse
    Sample type: Serum
  5. Abdel-Latif M. Diethylcarbamazine citrate ameliorates insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation. Int Immunopharmacol. 2015 Dec;29(2):607-12. doi: 10.1016/j.intimp.2015.09.021.
    Species: Mouse
    Sample type: Serum (outbred swiss albino mice treated with Diethylcarbamazine citrate for insulin resistence)
  6. Mandaliya DK, Patel S, Seshadri S. The Combinatorial Effect of Acetate and Propionate on High-Fat Diet Induced Diabetic Inflammation or Metaflammation and T Cell Polarization. Inflammation. 2021 Feb;44(1):68-79. doi: 10.1007/s10753-020-01309-7. PMID: 32978698.
    Species: Mouse
    Sample type: Serum
  7. Eo H., Jeon Yj., Lee M., et al. Brown Alga Ecklonia cava Polyphenol Extract Ameliorates Hepatic Lipogenesis, Oxidative Stress, and Inflammation by Activation of AMPK and SIRT1 in High-Fat Diet-Induced Obese Mice. J Agric Food Chem. 2014 Dec 22.
    Species: Mouse
    Sample type: Plasma (High-fat diet mice)

  8. Species: Mouse
    Sample type: Plasma
  9. Othman, Azza I., et al. "Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats." Biomedicine & Pharmacotherapy 94 (2017): 362-373.
    Species: Rat
    Sample type: Serum (diabetes)
  10. Majeed C., Al-Shammari A., Yausif E., et al. BM-MSCs Differentiated Insulin-Producing Cells Produce More Insulin in Presence of EGF than of FGF. Stem Cell Discovery 2015, 5, 33-39. http://dx.doi.org/10.4236/scd.2015.54004
    Species: Mouse
    Sample type: Conditioned Media (BM-MSC cells)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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